1.8 GHz微波对小鼠下丘脑组织结构的影响

doi:10.11843/j.issn.0366-6964.2020.03.021

Abstract

Abstract: The aim of this study was to investigate the morphological changes of hypothalamic cells in pre-puberty mice exposed to electromagnetic radiation at 1.8 GHz. The trial was randomly divided into three groups and each group consisted of 30 repetitions. Microwave irradiation frequency we used was 1.8 GHz and three groups were treated respectively on 0 (Control group), 1 (Low dose test group) and 2 mw·cm^-2 (High dose test group). Radiation was made on maternal mice during the entire period of gestation and lactation, and on the offspring until the day of postnatal 30. The mental state of mice were observed. The body mass, the cerebral mass of mice was recorded. Brain samples were collected at 30, 45 and 60 days of age. The histological changes of the hypothalamus were observed. The contents of neurotransmitter glutamate (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA) and glycine (Gly) in the hypothalamus were detected by HPLC. Results were as follows:(1) At the age of 30 days, the results showed that the body weight and brain weight in two experimental groups were lower than that of the control group (P<0.05), and the content of Asp, Glu, Gly and GABA in the brain of mice increased. Changes of ultrastructure including vacuolization in the hypothalamic cells, intracellular mitochondria edema, and dilated vascular space. (2) At the age of 45 days, the morphology of the cells in the low-dose test group recovered, but mitochondrial swelling still existed. Besides, the level of Asp and Gly in the brain decreased (P>0.05), and the level of Glu and GABA increased (P>0.05). In the high-dose test group, massive atrophic cells and swollen intracellular mitochondria still could be observed, and the content of Asp, Glu, Gly and GABA in the brain increased (P<0.05). (3) At the age of 60 days, the morphology of the cells in the low-dose group recovered. The mitochondria returned to normal, and there was no significant difference of the content of Asp, Glu, Gly and GABA in the brain between two groups (P>0.05). In the high-dose group, the hypothalamic cells basically recovered. Swollen mitochondria was nearly repaired and the nuclear was the normal appearance. The concentration of Asp in the brain decreased, and the concentration of Glu, GABA, and Gly increased. Overall, radiation damages the hypothalamic cells in mice, and these damages could be restored after radiation stops.

Key words: mice, 1.8 GHz electromagnetic wave, hypothalamus, neurotransmitters

CLC Number:

X503.22

AN Xiuxiu, LIU Lin, XU Yuwei, WU Yalin, LUO Mengyun, WEI Xueliang. Effect of 1.8 GHz Microwave on the Structure of Hypothalamus in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 612-619.

References 25

[1]	HERMANN D M, HOSSMANN K A. Neurological effects of microwave exposure related to mobile communication[J]. J Neurol Sci, 1997, 152(1):1-14.
[2]	陈光弟, 许正平, 姜槐. 移动电话射频电磁场与健康关系的体外试验研究[J]. 中华预防医学杂志, 2005, 39(4):285-287.CHEN G D, XU Z P, JIANG H. In vitro study on the relationship between radiofrequency electromagnetic field of mobile phone and health[J]. Chinese Journal of Preventive Medicine, 2005, 39(4):285-287. (in Chinese)
[3]	PETITDANT N, LECOMTE A, ROBIDEL F, et al. Cerebral radiofrequency exposures during adolescence:impact on astrocytes and brain functions in healthy and pathologic rat models[J]. Bioelectromagnetics, 2016, 37(5):338-350.
[4]	KESARI K K, BEHARI J. Fifty-gigahertz microwave exposure effect of radiations on rat brain[J]. Appl Biochem Biotechnol, 2008, 158(1):126-139.
[5]	KESARI K K, BEHARI J, KUMAR S. Mutagenic response of 2. 45 GHz radiation exposure on rat brain[J]. Int J Radiat Biol, 2010, 86(4):334-343.
[6]	KIVRAK E G, ALTUNKAYNAK B Z, ALKAN I, et al. Effects of 900-MHz radiation on the hippocampus and cerebellum of adult rats and attenuation of such effects by folic acid and Boswellia sacra[J]. J Microsc Ultrastruct, 2017, 5(4):216-224.
[7]	DESHMUKH P S, MEGHA K, NASARE N, et al. Effect of low level subchronic microwave radiation on rat brain[J]. Biomed Environ Sci, 2016, 29(12):858-867.
[8]	DE IULIIS G N, NEWEY R J, KING B V, et al. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro[J]. PLoS One, 2009, 4(7):e6446.
[9]	CHANCE W T, GROSSMAN C J, NEWROCK R, et al. Effects of electromagnetic fields and gender on neurotransmitters and amino acids in rats[J]. Physiol Behav, 1995, 58(4):743-748.
[10]	MEGHA K, DESHMUKH P S, RAVI A K, et al. Effect of low-intensity microwave radiation on monoamine neurotransmitters and their key regulating enzymes in rat brain[J]. Cell Biochem Biophys, 2015, 73(1):93-100.
[11]	SHARMA A, KESARI K K, SAXENA V K, et al. Ten gigahertz microwave radiation impairs spatial memory, enzymes activity, and histopathology of developing mice brain[J]. Mol Cell Biochem, 2017, 435(1-2):1-13.
[12]	沈向华, 潘登, 范奎奎, 等. Ghrelin通过弓状核区域α型雌二醇受体神经元介导雌性小鼠采食调控[J]. 畜牧兽医学报, 2017, 48(12):2399-2406.SHEN X H, PAN D, FAN K K, et al. Ghrelin mediate female mice food intake via affecting on estrogen receptor-alpha neuron in arcuate nucleus[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(12):2399-2406. (in Chinese)
[13]	BIRAN J, TAHOR M, WIRCER E, et al. Role of developmental factors in hypothalamic function[J]. Front Neuroanat, 2015, 9:47.
[14]	CHEN L J, SUN B H, CAO Y, et al. The effects of avermectin on amino acid neurotransmitters and their receptors in the pigeon brain[J]. Pestic Biochem Physiol, 2014, 110:13-19.
[15]	张红锋, 陈树德, 王耀发, 等. 脉冲电场对真皮成纤维细胞生长和膜流动性的影响[J]. 细胞生物学杂志, 1998, 20(2):82-85.ZHANG H F, CHEN S D, WANG Y F, et al. The effects of pulsed electric field on dermal fibroblast proliferation and membrane fluidity[J]. Chinese Journal of Cell Biology, 1998, 20(2):82-85. (in Chinese)
[16]	TESTYLIER G, TONDULI L, MALABIAU R, et al. Effects of exposure to low level radiofrequency fields on acetylcholine release in hippocampus of freely moving rats[J]. Bioelectromagnetics, 2002, 23(4):249-255.
[17]	JORGE-MORA T, MISA-AGUSTIÑO M J, RODRÍGUEZ-GONZÁLEZ J A, et al. The effects of single and repeated exposure to 2. 45 GHz radiofrequency fields on c-fos protein expression in the paraventricular nucleus of rat hypothalamus[J]. Neurochem Res, 2011, 36(12):2322-2332.
[18]	王水明. 电磁脉冲辐射对生殖系统的影响及其机制研究[D]. 北京:军事医学科学院, 2003.WANG S M. Effect of electromagnetic pulse irradiation on genital system and its mechani SM[D]. Beijing:Academy of Military Medical Sciences, 2003. (in Chinese)
[19]	RAWLS S M, GOMEZ T, STAGLIANOB G W, et al. Measurement of glutamate and aspartate in Planaria[J]. J Pharmacol Toxicol Methods, 2006, 53(3):291-295.
[20]	KISH P E, FISCHER-BOVENKERK C, UEDA T. Active transport of γ-aminobutyric acid and glycine into synaptic vesicles[J]. Proc Natl Acad Sci U S A, 1989, 86(10):3877-3881.
[21]	FONNUM F, MELTZER H Y. Psychopharmacology:the third generation of progress[M]. New York:Raven Press, 1987:173.
[22]	D'ANIELLO A. D-aspartic acid:an endogenous amino acid with an important neuroendocrine role[J]. Brain Res Rev, 2007, 53(2):215-234.
[23]	HASHIMOTO A, OKA T, NISHIKAWA T. Anatomical distribution and postnatal changes in endogenous free D-aspartate and D-serine in rat brain and periphery[J]. Eur J Neurosci, 1995, 7(8):1657-1663.
[24]	D'ANIELLO S, SOMORJAI I, GARCIA-FERNÀ-NDEZ J, et al. D-aspartic acid is a novel endogenous neurotransmitter[J]. FASEB J, 2011, 25(3):1014-1027.
[25]	SPINELLI P, BROWN E R, FERRANDINO G, et al. D-aspartic acid in the nervous system of Aplysia limacina:possible role in neurotransmission[J]. J Cell Physiol, 2006, 206(3):672-681.

Effect of 1.8 GHz Microwave on the Structure of Hypothalamus in Mice

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