Abstract: This experiment aimed to establish a method for simultaneous detection of multiple biogenic amines in pig intestinal chyme using liquid chromatography mass spectrometry (LC-MS). The experiment firstly optimized the conditions for derivatization of biogenic amines by dansyl chloride (DNS), and further determined the best method for purifying biogenic amines in intestinal chyme samples. The results showed that the derivatization conditions were optimal when the derivatization agent concentration was 8 mg·mL^-1 and the derivatization temperature was 40℃ for 90 min. The biogenic amine purification method was determined to be the best combination of extraction with ethyl acetate first and extraction with ethyl acetate after derivatization. The quantification results of 11 biogenic amines in the mass concentration range of 5-1 000 μg·L^-1 showed a good linear relationship (R²>0.99). The detection limit and limit of quantification were 0.01 - 0.23 μg·L^-1 and 0.03 - 0.76 μg·L^-1, respectively. The intra-day relative standard deviation of each bioamine was 0.73%-7.92%, and the inter-day relative standard deviation was 0.07%-8.67%. Finally, this method was used to detect biogenic amines in chyme samples from piglets' ileum, cecum, colon and rectum. The content distribution of 11 biogenic amines in different intestinal chyme was preliminarily determined. Detection of chyme samples in piglets' ileum, cecum, colon and rectum found that the biogenic amines in the chyme were mainly spermidine, putrescine, cadaverine, spermine and histamine. Tyramine, 1,7-diaminoheptane,serotonin, and agmatine were detected in very small amounts, and no tryptamine and phenethylamine were detected. In summary, the method established in this test has accurate results, high sensitivity, and good reproducibility, and can be used to detect multiple biogenic amines in chyme of different intestinal sections of piglets simultaneously.

Key words: intestinal chyme, biogenic amine, detection method, LC-MS