肉用绵羊二元杂交组合的筛选及CLPG基因遗传效应研究

doi:10.11843/j.issn.0366-6964.2020.01.007

Abstract

Abstract: In this study, in order to screen out the optimal meat sheep hybrid combination for suitable breeding in Tangshan area, Hebei Province, and to better guide production practices, three groups of meat sheep hybridization experiments were carried out, using AWF sheep, Dorper sheep and Charolais sheep as the male parent respectively, and the Small-tail Han sheep as the female parent. Among them, group 1 was the hybrid combination between AWF sheep and Small-tail Han sheep (AH group), group 2 was the hybrid combination between Dorper sheep and Small-tail Han sheep (DH group), and group 3 was the hybrid combination between Charolais sheep and Small-tail Han sheep (XH group). The growth performance and genetic effects of CLPG gene in hybrid progeny (F1) of 3 groups meat sheep were measured and analyzed. The results showed that under the same feeding conditions, birth weight of Xiahan ewes was slightly lower than that of Aohan ewes, the birth weight(ram), weaning weight, 6-month-old weight and average daily gain from birth to weaning of Xiahan hybrid progeny (F1) were the highest, and significantly or extremely significantly higher than those of the Duhan and Aoan hybrid progeny (F1) (P<0.05, P<0.01). The average daily gain from weaning to 6-month-old of Duhan hybrid progeny (F1 rams and ewes) were the highest, followed by Xiahan hybrid progeny (F1 rams and ewes), and the difference between Duhan hybrid progeny (F1 rams and ewes) and the other two groups was not significant(P>0.05). Except for body length and chest width at 3-month-old, and body length and body height at 6-month-old, the residual body size indexes of Xiahan hybrid progeny (F1) were the highest, and some of them were extremely significantly higher than those of Aohan hybrid progeny (F1) (P<0.01). The above results indicate that the Xiahan hybird progeny (F1) had a higher basic body weight and a faster growth rate after birth,while Duhan hybrid progeny (F1) showed a faster growth rate after weaning. The genetic effects analysis of CLPG gene showed that there was a missense mutation site at the 41 bp for 3 meat sheep hybrid progeny(F1) populations. There were CC and CT genotypes at this mutation site. The CC genotype was dominant genotype, and C allele was dominant allele. The least squares analysis of genotype and growth traits showed that birth weight, weaning weight, 6-month-old weight, 3-month-old body size indexes and 6-month-old body size indexes of individuals with CT genotype were significantly higher than those of individuals with CC genotypes. Based on the above results, it is suggested that the hybridization advantage between Charolais sheep and Small-tail Han sheep is obvious. T allele can promote the growth and development of meat sheep. It is recommended to choose the Xiahan hybrid combination and the individual carrying T allele when organizing meat sheep production in Tangshan area, Hebei Province.

Key words: meat sheep, hybrid combination, growth performance, CLPG gene, genetic effect

CLC Number:

S826.2

LIU Yanjun, WANG Jiantao, LIU Zhengzhu, WANG Guizhu, ZHENG Yingzhen, JIA Jingliang, Lü Jianguo, WANG Guosen, LI Xianglong, GONG Yuanfang. Screening of Hybrid Combinations and Genetic Effects Analysis of CLPG Gene in Meat Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 55-63.

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Screening of Hybrid Combinations and Genetic Effects Analysis of CLPG Gene in Meat Sheep

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