广东省零售市场鸡肉中肯塔基沙门菌的流行情况及耐药性分析

doi:10.11843/j.issn.0366-6964.2019.12.015

Abstract

Abstract: The aim of this study was to understand the prevalence, drug resistance and drug resistance genes carried by Salmonella Kentucky in the chickens from the retail markets in Guangdong province. The chicken samples collected from five prefecture-level cities of Guangdong in 2016 were selected to isolate Salmonella strains.Then the Salmonella serotypes were identified. Drug sensitivity test, drug resistance gene detection and molecular typing of the isolated S. Kentucky were carried out. As a result, 152 strains of Salmonella were isolated from 245 chicken samples, with the positive rate of 62.04% (152/245). Nineteen serotypes were identified, of which the main serotypes were S. Agona (29/152, 19.08%), S. Corvallis (25/152, 16.45%) and S. Kentucky (20/152, 13.16%). The drug sensitivity test of S. Kentucky showed that they were highly resistant to sulfafurazole (100%), nalidixic acid (90%), tetracycline (75%), ampicillin (65%), ceftazidime (55%), and ciprofloxacin (55%). Eighty-five percent (17/20) of the strains were resistant to three or more antimicrobial agents. The detection of quinolone resistance genes presented gyrA mutations (Ser83Phe, Asp87Asn and Asp87Gly) in 95% (19/20) of the strains, including double mutations in gyrA (Ser83Phe and Asp87Asr, Ser83Phe and Asp87Gly) in 57.89% (11/19) of the strains and triple mutation in gyrA in 5.26% (1/19) of the strains. In addition, parC mutations occurred in 100% (20/20) of the strains (Tyr62Ser and Ser85Ile). Forty-five percent of the isolates (9/20) carried plasmid-mediated quinolone resistance (PMQR) genes (aac(6')-Ib-cr, qnrB, qnrS, oqxAB), with aac(6')-Ib-cr resistance gene as the most common one. The detection rates of β-lactam resistance genes, encompassing bla_TEM-1, bla_OXA-1 and bla_CTX-M-55, were 25%, 10% and 5%, respectively. The results of cluster analysis of pulsed field gel electrophoresis (PFGE) map unveiled the genetic relationship between the serotypes of S. Kentucky, their genetic diversity, and high homology in some strains. It was found that S. Kentucky was one of the major epidemics in the retail chicken meat in Guangdong. It was more resistant to traditional drugs such as sulfisoxazole, nalidixic acid, tetracycline and ampicillin, and was particularly resistant to ciprofloxacin and ceftazidime, featuring multiple and multi-drug resistant phenotypes. S. Kentucky characterized high mutation rates in the quinolone resistance-determining regions of gyrA and parC. Molecular typing revealed the possibility of cross-regional transmission between strains, underpinning the studies on the traceability of S. Kentucky.

Key words: Salmonella Kentucky, antibiotic resistance, resistance genes, molecular typing

CLC Number:

S852.61

WANG Shaojun, SUN Kangtai, XIONG Zhiying, QU Xiaoyun, MA Yeben, CHEN Zhengquan, ZHANG Hongxia, WEN Junping, BAI Jie, ZHAN Zeqiang, GAO Yuan, WEI Yihuan, LIAO Ming, ZHANG Jianmin. Analysis of Prevalence and Drug Resistance of Salmonella Kentucky in Chicken from Retail Markets in Guangdong[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2509-2517.

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Analysis of Prevalence and Drug Resistance of Salmonella Kentucky in Chicken from Retail Markets in Guangdong

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