硫酸铜溶液诱导对耐甲氧西林金黄色葡萄球菌抗菌药物抗性效应的初步研究

doi:10.11843/j.issn.0366-6964.2020.04.021

Abstract

Abstract: The study aimed to investigate the effects of copper sulfate solution continuous induction on the antibiotic resistance ability and the expression of related resistance genes of methicillin-resistant Staphylococcus aureus(MRSA) isolates. The minimum inhibitory concentrations(MICs) of MRSA strains (MR-YB1224, MR-YS3 and MR-P318) to copper sulfate, β-lactam(including ampicillin, oxacillin and cefoxitin), fluoroquinolones(including ofloxacin), macrolides(including roxithromycin) and aminoglycosides(including streptomycin) were determined using the standard broth microdilution method. A quantitative real time-PCR was applied to monitor relative expression levels of anti-heavy metal genes (copA, arsB) and antibiotic resistance genes (mecA, norA, ermB, aac6'/aph2″). After continuous induction by copper sulfate solution for 7 days, the MIC values to copper sulfate solution increased for three MRSA strains; especially the MIC values to β-lactam antibiotics significantly increased after induction (P<0.05). The change of MIC values to the different antibiotics were as follows:oxacillin > ampicillin > cefoxitin > ofloxacin > streptomycin > roxithromycin. In addition, the expression of the anti-heavy metal genes and related antibiotic resistance genes for the three MRSA strains were significantly up-regulated (P<0.05). The results show that copper sulfate solution has a strong synergistic effect on the antibiotic resistance of MRSA strains.

Key words: Staphylococcus aureus, copper sulfate solution, induction, antibiotic resistance, anti-heavy metal genes, RT-PCR

CLC Number:

S825.611

LUO Mengyou, TANG Cheng, ZHAO Yanying, CHEN Juan, TANG Junni. The Study of Copper Sulfate Solution Induction on the Resistance Effect of Methicillin-resistant Staphylococcus aureus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 841-850.

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The Study of Copper Sulfate Solution Induction on the Resistance Effect of Methicillin-resistant Staphylococcus aureus

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