运输应激对山羊心的病理损伤及HSP27、HSP70和HSP90表达的影响

doi:10.11843/j.issn.0366-6964.2019.10.020

Abstract

Abstract: The effects of different transport time on pathological damage in the heart of goats (Capra hircus) were investigated with HE staining and transmission electron microscopy technique, and the expression of heat shock protein were analyzed with immunohistochemistry, Western blot and ELISA. The changes of microstructure, ultrastructure, localization and expression of HSP70, HSP27 and HSP90 in goat hearts under 2 and 6 hours of road transport stress were analyzed. The results showed that the myocardial fibers of goats in transport stress group exhibited diffuse granular degeneration of varying degrees, the intracellular mitochondria were swollen and broken, the edema, hemorrhage and inflammatory cell infiltration were observed in intercellular substance, capillaries were dilated and congested. And the lesion in the group transported for six hours was more serious. HSP70, HSP27 and HSP90 were mainly expressed in the cytoplasm of cardiac myocytes. HSP27 and HSP90 were also expressed in the nucleus of cardiac myocytes. This three proteins were unevenly distributed in myocardial fibers and myocardial cells of different regions. Compared with the control group, the expression levels of HSP27 protein in the heart of goats in group transported for two hours increased significantly, while the expression level of HSP27 after six-hour transportation stress decreased. Compared with the control group, the expression level of HSP70 and HSP90 protein in the groups transported for 2 and 6 hours increased significantly or increased extremely significantly. In conclusion, the pathological damage of cardiac tissue cells occurred in goats under transport stress, and the acute degeneration of cardiac cells was very obvious. The degeneration was more serious after six-hour transportation stress. The expression levels of HSP70, HSP27 and HSP90 proteins changed significantly after transport, which was related to the stress injury of heart.

CLC Number:

S852.3

ZHENG Wenya, ZHANG Wenyuan, LIU Ben, YANG Xue, XIAO Peng, WANG Anping, CUI Yan. Effects of Transport Stress on Pathological Injury and Expression of HSP27, HSP70 and HSP90 of the Heart in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2129-2138.

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Effects of Transport Stress on Pathological Injury and Expression of HSP27, HSP70 and HSP90 of the Heart in Goats

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