细胞传代致弱的鹅细小病毒YG毒株致病性变化的研究

doi:10.11843/j.issn.0366-6964.2016.01.018

Abstract

Abstract:

This experiment was conducted to study pathogenicity changes in susceptible goslings of cell attenuated goose parvovirus YG strain.To obtain attenuated vaccine strain，goose parvovirus(GPV) YG strain was propagated alternately in goose embryo fibroblasts(GEF) and duck embryo fibroblasts(DEF).Cells infected with YG strain began to induce cytopathogenic effect after 12 passages.Results of indirect immunofluorescence assay(IFA) showed that the numbers of infected cells with green fluorescence increased with the time of infection，and the highest number of positive cells were obtained at 96 h post-infection.Virus titers increased with serial passages going and virus titer reached 10^6.5 TCID₅₀•mL^-1 after passage 50.Compared to parental virus，the mortality and morbidity of goslings decreased obviously after challenged with virus passage 50.There were no observed characterized pathogenic changes or clinical symptoms of Derzsy’s disease in goslings after challenged with passage 50 YG virus.

CLC Number:

S852.659.6

SHAOZHOU Wu-lin，LI Chen-xi，LIU Ming，ZHANG Qing-shan，ZHANG Yun. Pathogenicity Changes of Cell Attenuated Goose Parvovirus YG Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 135-140.

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Pathogenicity Changes of Cell Attenuated Goose Parvovirus YG Strain

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