鸡J亚群禽白血病病毒AH-J11株的全基因组感染性克隆构建

doi:10.11843/j.issn.0366-6964.2013.12.017

Abstract

Abstract:

In order to explore the pathogenesis of subgroup J avian leukosis virus(ALV-J), a full-length infectious clone of ALV-J (pSK-AH-J11) was constructed by combining of four fragments using PCR method from AH-J11 isolated from broiler in Anhui province, then the complete genome was cloned into pBluescript Ⅱ SK(+) vector. The recombinant plasmid pBl-AH-J11 was transfected into chicken embryo fibroblast (CEF) cells and the recombinant ALV-J was rescued. The rescued ALV-J (rALV-J) was identified by RT-PCR, Western blot, indirect immunofluorescence assay and sequence comparision of gp85 gene with AH-J11strain, respectively. The results showed that the recombinant rALV-J was rescued in CEF. This study provides a useful platform for investigation of the pathogenesis and molecular biology of ALV-J.

CLC Number:

S852.659.3

WANG Bei, LIU Fang, WANG Han-qing, LI Ning, WEI Jian-zhong, LIU Guang-qing, WANG Gui-jun. Construction of Infectious Clone of Avian Leucosis Virus Subgroup J AH-J11 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1976-1981.

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References

［1］PAYNE L N, S R BROWN, N BUMSTEAD, et al. A novel subgroup of exogenous avian leucosis virus in chickens［J］. J Gen Virol, 1991, 72(Pt 4): 801-807.

［2］王建新，崔治中，张纪元,等．J亚群禽自血病病毒与禽网状内皮增生症病毒共感染对肉鸡生长和免疫功能的抑制作用［J］．中国兽医学报，2003，23(3)：211-213.

［3］刘绍琼, 王波, 张振杰, 等. 817肉杂鸡肉瘤组织分离出A、J亚型禽白血病病毒［J］. 畜牧兽医学报，2011, 42(3): 396-401.

［4］CUI Z, DU Y, ZHANG Z, et al. Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains ［J］. Avian Dis, 2003, 47(4):1321-1330.

［5］XU B, DONG W, YU C, et al. Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China ［J］. Avian Pathol, 2004, 33(1): 13-17.

［6］SUN S, CUI Z. Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local "yellow" chickens ［J］. Avian Pathol, 2007, 36(3): 221-226.

［7］倪伟, 秦立廷, 孙洪磊, 等. 某海兰褐鸡场祖代、父母代及商品代鸡血管瘤型ALV-J的分离与鉴定［J］. 畜牧兽医学报, 2011, 42(9): 1341-1345.

［8］张振杰, 刘少琼, 王波, 等. 地方品种皖南黄肉种鸡ALV-J与REV的共感染及其分子变异分析［J］. 中国农业科学, 2011, 44(11):2379-2386.

［9］张贺楠, 齐岩, 史伟伟. 不同病变型J亚群禽白血病病毒LTR启动子序列分析及活性比较［J］. 病毒学报, 2010, 26(5): 402-406.

［10］张纪元，崔治中，丁家波，等.J亚群白血病病毒NXO101株感染性克隆化病毒的构建及其致病性［J］. 微生物学报，2005，45(3):437-440.

［11］张贺楠，赖汉漳，梁雁萍，等.血管瘤病变型J亚群禽白血病CHN株感染性克隆的构建［C］.// 中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第七次研讨会论文集.哈尔滨，2008.

［12］王超，缪华先，谢宝婵，等.J亚型禽白血病病毒的拯救与鉴定［J］. 中国预防兽医学报，2011，35(11):837-840.

［13］林艳，夏静，邹年莉, 等. 1株髓细胞瘤型J亚群禽白血病病毒感染性克隆的构建与病毒拯救［J］. 畜牧兽医学报，2013，44(5): 754-759.

［14］纪晓琳，王琦，高玉龙, 等. J亚群禽白血病病毒分离株HLJ09SH01株感染性克隆的构建及其致病性研究［J］. 中国预防兽医学报，2013, 35(1): 15-18.

［15］王琦，王永强，康忠惠，等.蛋鸡J亚群禽白血病病毒分离株SD1009株感染性克隆的构建与病毒拯救［J］. 中国兽医科学，2011，41(11):1111-1116.

［16］萨姆布鲁克 J, 拉塞尔D W. 分子克隆实验指南［M］. 黄培堂，王嘉玺，朱厚础，等译.3版.北京：科学出版社，2002.

［17］高玉龙，贠炳岭，秦立廷, 等. 蛋鸡J 亚群禽白血病病毒3′非编码区序列特征分析［J］. 畜牧兽医学报，2012, 43(11):1841-1846.

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Construction of Infectious Clone of Avian Leucosis Virus Subgroup J AH-J11 Strain

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