GADD45β蛋白抑制ALV-J在DF-1细胞中的复制

doi:10.11843/j.issn.0366-6964.2021.06.020

Abstract

Abstract: Growth arrest and DNA damage 45β (GADD45β) is involved in various cellular signaling pathways and plays an important role in viral infection, but there are few studies on avian leukosis virus (ALV) infection. In this study, we used RT-qPCR and Western blot to detect the expression level of GADD45β in DF-1 cells infected with ALV-J. To investigate the effect of GADD45β on the replication of ALV-J, immunofluorescence, Western blot and ELISA were used to detect the effect of overexpression of GADD45β and interference of GADD45β on ALV-J replication. The results showed that the protein level of ALV-J was decreased after overexpression of GADD45β. However, when interfering the expression of GADD45β, the replication of ALV-J showed the opposite effect, indicating that GADD45β inhibited the replication of ALV-J. This study found for the first time that GADD45β has the effect of inhibiting the replication of ALV-J, which is of great significance and value for ALV-J-related research.

Key words: growth arrest and DNA damage 45β, GADD45β, ALV-J, virus replication

CLC Number:

S852.659.3

CHEN Sheng, LIAO Zhihong, XIE Zi, CHEN Feng, XIE Qingmei, SHU Wei. GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1662-1669.

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GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells

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