不同等级牛卵丘-卵母细胞复合体体外成熟后卵母细胞发育能力差异的机制分析

doi:10.11843/j.issn.0366-6964.2025.09.025

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (9): 4432-4451.doi: 10.11843/j.issn.0366-6964.2025.09.025

不同等级牛卵丘-卵母细胞复合体体外成熟后卵母细胞发育能力差异的机制分析

王蕊(), 衡诺, 胡樱凡, 王欢, 朱妮, 何维, 轩秀丽, 胡智辉, 熊铿, 巩建飞, 郝海生, 朱化彬, 赵善江*()

中国农业科学院北京畜牧兽医研究所, 农业农村部畜禽生物育种重点实验室, 北京 100193

收稿日期:2025-03-11 出版日期:2025-09-23 发布日期:2025-09-30
通讯作者: 赵善江 E-mail:ruiwang202010@163.com;zhaoshanjiang@caas.cn
作者简介:王蕊(1999-)，女，安徽马鞍山人，硕士，主要从事动物遗传育种与繁殖研究，E-mail: ruiwang202010@163.com
基金资助:
国家奶牛产业技术体系项目(CARS-36)；云南省重大科技专项计划(202402AE090034)；河北省现代农业产业技术体系建设专项资金(HBCT2024230203)；宁夏回族自治区重点研发计划(2021BEF02023)；中国农业科学院科技创新工程(ASTIP-IAS06)

Mechanisms of Developmental Competence Differences of Oocytes from Different Grades of Bovine Cumulus-Oocyte Complexes after in Vitro Maturation

WANG Rui(), HENG Nuo, HU Yingfan, WANG Huan, ZHU Ni, HE Wei, XUAN Xiuli, HU Zhihui, XIONG Keng, GONG Jianfei, HAO Haisheng, ZHU Huabin, ZHAO Shanjiang*()

Key Laboratory of Livestock and Poultry Biological Breeding of the Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2025-03-11 Online:2025-09-23 Published:2025-09-30
Contact: ZHAO Shanjiang E-mail:ruiwang202010@163.com;zhaoshanjiang@caas.cn

摘要/Abstract

摘要：

旨在探究不同等级牛卵丘-卵母细胞复合体(cumulus-oocyte complexes, COCs)体外成熟后卵母细胞发育能力差异的分子机制。本试验以屠宰场卵巢来源的牛COCs为研究对象，参照国际胚胎技术学会手册根据卵丘细胞层数、卵丘细胞紧密程度和卵母细胞细胞质外观将牛COCs分为一二级(较好)和三四级(较差)两组，每组3次重复，每个重复组含100枚以上COCs，进行体外成熟培养。本研究通过Smart-seq2技术对成熟前后的一二级和三四级COCs的卵母细胞进行了转录组测序并对差异基因进行了分析验证，利用非靶向代谢组学技术对不同级别COCs的培养液进行了差异代谢物分析。结果显示：1)三四级COCs的卵母细胞体外成熟、受精后的卵裂率和囊胚率(60.59%±6.54%，22.11%±2.79%)显著低于一二级(78.09%±3.01%，32.56%± 4.33%，P＜0.05)；2)Smart-seq测序结果显示，体外成熟前一二级和三四级COCs的卵母细胞中共筛选出541个显著差异表达基因(DEGs)，体外成熟22~24 h(体外成熟后)一二级和三四级卵母细胞中共筛选出860个显著DEGs，GO和KEGG富集分析结果显示，这些DEGs主要与糖代谢、氧化磷酸化信号通路相关；3)数字PCR结果显示，体外成熟前后三四级COCs的卵母细胞中糖酵解相关基因PKM和电子传递链复合物I相关抑制基因NDUFA4L2的拷贝数水平均显著低于一二级(P＜0.01)，体外成熟后三四级卵母细胞中电子供体生成相关基因IDH3α的拷贝数水平显著低于一二级(P＜0.05)；同样体外成熟前后三四级COCs的卵丘细胞中NDUFA4L2 mRNA表达水平显著低于一二级(P＜0.05)，但是PKM和IDH3α mRNA表达水平却显著高于一二级(P＜0.05，P＜0.01)；4)代谢物分析结果显示，不同级别COCs的培养液中共鉴定出69种显著上调差异代谢物，71种显著下调差异代谢物，功能富集分析发现差异代谢物主要富集在三羧酸循环(TCA)、糖酵解/糖异生等代谢通路上，进一步对差异代谢物(α-D-葡萄糖、丙酮酸、乳酸)进行相对丰度分析发现三四级COCs成熟液中α-D-葡萄糖含量显著高于一二级(P＜0.01)。5)在三四级COCs体外成熟液中添加糖酵解产物丙酮酸钠能够显著提高三四级COCs体外成熟、受精后的卵裂率(72.54%±4.79% vs. 61.71%±4.75%)和囊胚率(30.63%±4.15% vs. 22.72%±2.39%，P＜0.05)。综上，本研究表明三四级COCs的卵母细胞体外成熟后发育能力差可能是由于其本身糖酵解活性较弱，加之其外围卵丘细胞能量底物供给不足，导致卵母细胞成熟时能量不足，发育能力受限；通过添加丙酮酸钠能够明显改善三四级COCs的卵母细胞体外成熟后发育能力，研究结果为提高COCs利用率和胚胎生产效率提供了理论依据。

关键词: 牛, 卵丘卵母细胞复合体, 体外成熟, 糖代谢, 丙酮酸钠

Abstract:

This study aimed to investigate the molecular mechanisms underlying the differences in developmental competence of oocytes from different grades of bovine cumulus-oocyte complexes (COCs) after in vitro maturation. Bovine COCs derived from ovaries of bovine from slaughterhouse were classified into two groups (grades Ⅰ-Ⅱ (better)and grades Ⅲ-Ⅳ (poor)) based on cumulus cell layers, compactness, and cytoplasm appearance according to the International Embryo Technology Society manual. The experiment was divided into 3 replicates, each replicate group contained more than 100 COCs. After in vitro maturation, transcriptome analysis of oocytes was performed using Smart-seq2 technology and the differentially expressed genes (DEGs) were validated. Non-targeted metabolomics was employed to analyze differential metabolites in the culture medium of different COC grades. The results showed that: 1) Grade Ⅲ-Ⅳ COCs exhibited significantly lower cleavage rate (60.59%±6.54%) and blastocyst rate (22.11%±2.79%) compared to grade Ⅰ-Ⅱ COCs (78.09%±3.01%, 32.56%±4.33%, P < 0.05); 2) Smart-seq2 analysis identified 541 significant DEGs between grade Ⅰ-Ⅱ and grade Ⅲ-Ⅳ oocytes before maturation, and 860 DEGs after 22-24 h of maturation (after in vitro maturation). GO and KEGG enrichment analyses revealed that these DEGs were primarily associated with glucose metabolism and oxidative phosphorylation pathways; 3) Digital PCR demonstrated significantly lower copy numbers of glycolysis-related gene PKM and electron transport chain complex Ⅰ inhibitor gene NDUFA4L2 in grade Ⅲ-Ⅳ oocytes compared to grade Ⅰ-Ⅱ (P < 0.01). After maturation, grade Ⅲ-Ⅳ oocytes showed significantly lower copy numbers of electron donor generation-related gene IDH3α compared to grade Ⅰ-Ⅱ (P < 0.05). Interestingly, grade Ⅲ-Ⅳ cumulus cells exhibited significantly lower NDUFA4L2 mRNA expression (P < 0.05) but higher PKM and IDH3α mRNA expression than grade Ⅰ-Ⅱ (P < 0.05, P < 0.01); 4) Metabolite analysis identified 69 significantly upregulated and 71 downregulated metabolites in different grades of bovine cumulus-oocyte complexes culture medium. Further functional enrichment analysis showed these metabolites were primarily involved in tricarboxylic acid cycle (TCA), and glycolysis/gluconeogenesis pathways. Relative abundance analysis of key metabolites (α-D-glucose, pyruvate, lactate) revealed significantly higher α-D-glucose content in grade Ⅲ-Ⅳ maturation medium (P < 0.01); 5) Supplementation of sodium pyruvate, a glycolysis product, significantly improved cleavage rate (72.54%±4.79% vs. 61.71%±4.75%) and blastocyst rate (30.63%±4.15% vs. 22.72%±2.39%, P < 0.05) in grade Ⅲ-Ⅳ COCs. This study suggests that the poor developmental competence of grade Ⅲ-Ⅳ COCs after in vitro maturation may result from insufficient glycolytic activity in oocytes coupled with insufficient energy substrate supply from surrounding cumulus cells. The addition of sodium pyruvate significantly improved the developmental competence of grade Ⅲ-Ⅳ COCs, which provide a theoretical basis for enhancing COC utilization and embryo production efficiency.

Key words: bovine, cumulus-oocyte complex, in vitro maturation, glucose metabolism, sodium pyruvate

中图分类号:

S823.3

王蕊, 衡诺, 胡樱凡, 王欢, 朱妮, 何维, 轩秀丽, 胡智辉, 熊铿, 巩建飞, 郝海生, 朱化彬, 赵善江. 不同等级牛卵丘-卵母细胞复合体体外成熟后卵母细胞发育能力差异的机制分析[J]. 畜牧兽医学报, 2025, 56(9): 4432-4451.

WANG Rui, HENG Nuo, HU Yingfan, WANG Huan, ZHU Ni, HE Wei, XUAN Xiuli, HU Zhihui, XIONG Keng, GONG Jianfei, HAO Haisheng, ZHU Huabin, ZHAO Shanjiang. Mechanisms of Developmental Competence Differences of Oocytes from Different Grades of Bovine Cumulus-Oocyte Complexes after in Vitro Maturation[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4432-4451.

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参考文献 43

1	VIANA J H . 2023 Statistics of embryo production and transfer in domestic farm animals[J]. Embr Tech Newsl, 2024, 42 (4): 31- 46.
2	KRISHER R L . The effect of oocyte quality on development[J]. J Anim Sci, 2004, 82 (E-Suppl): E14- E23.
3	HOMER H A . The role of oocyte quality in explaining "Unexplained" infertility[J]. Semin Reprod Med, 2020, 38 (1): 21- 28. doi: 10.1055/s-0040-1721377
4	GALLO A , ESPOSITO M C , BONI R , et al. Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy[J]. Biol Res, 2022, 55 (1): 34. doi: 10.1186/s40659-022-00403-4
5	JEENA L M , KUMAR D , RAHANGDALE S , et al. Effect of cumulus cells of cumulus-oocyte complexes on in vitro maturation, embryonic developmental and expression pattern of apoptotic genes after in vitro fertilization in water buffalo (Bubalus bubalis)[J]. Anim Biotechnol, 2020, 31 (2): 135- 141. doi: 10.1080/10495398.2018.1554580
6	DE LOOS F , VAN VLIET C , VAN MAURIK P , et al. Morphology of immature bovine oocytes[J]. Gamete Res, 1989, 24 (2): 197- 204. doi: 10.1002/mrd.1120240207
7	RODRIGUEZ-VARELA C , HERRAIZ S , LABARTA E . Mitochondrial enrichment in infertile patients: a review of different mitochondrial replacement therapies[J]. Ther Adv Reprod Health, 2021, 15, 1343248568.
8	HAO X , ZHAO J , RODRIGUEZ-WALLBERG K A . Comprehensive atlas of mitochondrial distribution and dynamics during oocyte maturation in mouse models[J]. Biomark Res, 2024, 12 (1): 125. doi: 10.1186/s40364-024-00672-z
9	YUN Y , LEE S , SO C , et al. Oocyte development and quality in young and old mice following exposure to atrazine[J]. Environ Health Perspect, 2022, 130 (11): 117007. doi: 10.1289/EHP11343
10	PETERS A E , FORD E A , ROMAN S D , et al. Impact of Bisphenol A and its alternatives on oocyte health: a scoping review[J]. Hum Reprod Update, 2024, 30 (6): 653- 691. doi: 10.1093/humupd/dmae025
11	安卓, 谢聪聪, 赵明, 等. 线粒体对卵母细胞发育的影响[J]. 生殖医学杂志, 2021, 30 (11): 1533- 1537.
	AN Z , XIE C C , ZHAO M , et al. Eeffects of mitochondria on oocyte development[J]. Journal Reproductive Medicine, 2021, 30 (11): 1533- 1537.
12	XIONG Y Y , ZHU H Y , SHI R J , et al. Regulation of glucose metabolism: Effects on oocyte, preimplantation embryo, assisted reproductive technology and embryonic stem cell[J]. Heliyon, 2024, 10 (19): e38551. doi: 10.1016/j.heliyon.2024.e38551
13	KOBAYASHI H , YOSHIMOTO C , MATSUBARA S , et al. Altered energy metabolism, mitochondrial dysfunction, and redox imbalance influencing reproductive performance in granulosa cells and oocyte during aging[J]. Reprod Sci, 2024, 31 (4): 906- 916. doi: 10.1007/s43032-023-01394-7
14	NAHAR A , BECKER J , PASQUARIELLO R , et al. FGF2, LIF, and IGF-1 supplementation improves mouse oocyte in vitro maturation via increased glucose metabolismdagger[J]. Biol Reprod, 2024, 110 (4): 672- 683. doi: 10.1093/biolre/ioae014
15	ZHANG Q , REN J , WANG F , et al. Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3[J]. Free Radic Biol Med, 2022, 187, 1- 16. doi: 10.1016/j.freeradbiomed.2022.05.010
16	PICELLI S , FARIDANI O R , BJORKLUND A K , et al. Full-length RNA-seq from single cells using Smart-seq2[J]. Nat Protoc, 2014, 9 (1): 171- 181. doi: 10.1038/nprot.2014.006
17	OU Z , DU J , LIU N , et al. The impact of low oocyte maturity ratio on blastocyst euploidy rate: a matched retrospective cohort study[J]. Contracept Reprod Med, 2024, 9 (1): 41. doi: 10.1186/s40834-024-00303-w
18	PARRELLA A , IRANI M , KEATING D , et al. High proportion of immature oocytes in a cohort reduces fertilization, embryo development, pregnancy and live birth rates following ICSI[J]. Reprod Biomed Online, 2019, 39 (4): 580- 587. doi: 10.1016/j.rbmo.2019.06.005
19	XIE H L , WANG Y B , JIAO G Z , et al. Effects of glucose metabolism during in vitro maturation on cytoplasmic maturation of mouse oocytes[J]. Sci Rep, 2016, 6, 20764. doi: 10.1038/srep20764
20	ALVAREZ G M , BARRIOS E M , ELIA E , et al. Effects of gonadotrophins and insulin on glucose uptake in the porcine cumulus-oocyte complex during ⅣM[J]. Reprod Fertil Dev, 2019, 31 (8): 1353- 1359. doi: 10.1071/RD18321
21	ABDULHASAN M K , LI Q , DAI J , et al. CoQ10 increases mitochondrial mass and polarization, ATP and Oct4 potency levels, and bovine oocyte MII during ⅣM while decreasing AMPK activity and oocyte death[J]. J Assist Reprod Genet, 2017, 34 (12): 1595- 1607. doi: 10.1007/s10815-017-1027-y
22	ZHANG Y , JIANG X , SONG X , et al. Mendelian randomization and multi-omics approach analyses reveal impaired glucose metabolism and oxidative phosphorylation in visceral adipose tissue of women with polycystic ovary syndrome[J]. Hum Reprod, 2024, 39 (12): 2785- 2797. doi: 10.1093/humrep/deae244
23	BOLAND N I , HUMPHERSON P G , LEESE H J , et al. The effect of glucose metabolism on murine follicle development and steroidogenesis in vitro[J]. Hum Reprod, 1994, 9 (4): 617- 623. doi: 10.1093/oxfordjournals.humrep.a138559
24	PROCHOWNIK E V , WANG H . The metabolic fates of pyruvate in normal and neoplastic cells[J]. Cells, 2021, 10 (4): 762. doi: 10.3390/cells10040762
25	LI Y R , PENG R R , GAO W Y , et al. The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality[J]. Aging (Albany NY), 2019, 11 (4): 1110- 1128.
26	LIU Z , CHAILLOU T , SANTOS A E , et al. Mitochondrial NDUFA4L2 is a novel regulator of skeletal muscle mass and force[J]. FASEB J, 2021, 35 (12): e22010.
27	CHEN Y C , LI J Y , LI C J , et al. Luteal phase ovarian stimulation versus follicular phase ovarian stimulation results in different human cumulus cell genes expression: A pilot study[J]. Int J Med Sci, 2021, 18 (7): 1600- 1608. doi: 10.7150/ijms.55955
28	HOU J Y , WANG X L , CHANG H J , et al. PTBP1 crotonylation promotes colorectal cancer progression through alternative splicing-mediated upregulation of the PKM2 gene[J]. J Transl Med, 2024, 22 (1): 995. doi: 10.1186/s12967-024-05793-5
29	WEN J , WANG G L , YUAN H J , et al. Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes[J]. Sci Rep, 2020, 10 (1): 2782. doi: 10.1038/s41598-020-59709-6
30	SUTTON-MCDOWALL M L , GILCHRIST R B , THOMPSON J G . The pivotal role of glucose metabolism in determining oocyte developmental competence[J]. Reproduction, 2010, 139 (4): 685- 695. doi: 10.1530/REP-09-0345
31	赵昍朋, 李宝山, 程东凯. 颗粒细胞糖脂代谢对卵母细胞发育影响的研究进展[J]. 妇儿健康导刊, 2024, 3 (15): 22- 26.
	ZHAO X P , LI B S , CHENG D K . Research progress on the effects of granulosa cell glucose and lipid metabolism on oocyte development[J]. Fuer Jiankang Daokan, 2024, 3 (15): 22- 26.
32	DALTON C M , SZABADKAI G , CARROLL J . Measurement of ATP in single oocytes: impact of maturation and cumulus cells on levels and consumption[J]. J Cell Physiol, 2014, 229 (3): 353- 361. doi: 10.1002/jcp.24457
33	SHI L , WANG H , ZHU S , et al. Multi-omics reveal the metabolic changes in cumulus cells during aging[J]. Cell Prolif, 2025, e70014.
34	ZHANG Q , HAO J X , LIU B W , et al. Supplementation of mitochondria from endometrial mesenchymal stem cells improves oocyte quality in aged mice[J]. Cell Prolif, 2023, 56 (3): e13372. doi: 10.1111/cpr.13372
35	MEI N H , GUO S M , ZHOU Q , et al. H3K4 methylation promotes expression of mitochondrial dynamics regulators to ensure oocyte quality in mice[J]. Adv Sci (Weinh), 2023, 10 (12): e2204794. doi: 10.1002/advs.202204794
36	ZHANG Y , LIU L , YIN T L , et al. Follicular metabolic changes and effects on oocyte quality in polycystic ovary syndrome patients[J]. Oncotarget, 2017, 8 (46): 80472- 80480. doi: 10.18632/oncotarget.19058
37	MOORE S G , FAIR T , LONERGAN P , et al. Genetic merit for fertility traits in Holstein cows: Ⅳ. Transition period, uterine health, and resumption of cyclicity[J]. J Dairy Sci, 2014, 97 (5): 2740- 2752. doi: 10.3168/jds.2013-7278
38	CAI X , NG C P , JONES O , et al. Lactate activates the mitochondrial electron transport chain independently of its metabolism[J]. Mol Cell, 2023, 83 (21): 3904- 3920. doi: 10.1016/j.molcel.2023.09.034
39	LIU K , WEI H , NONG W , et al. Nampt/SIRT2/LDHA pathway-mediated lactate production regulates follicular dysplasia in polycystic ovary syndrome[J]. Free Radic Biol Med, 2024, 225, 776- 793. doi: 10.1016/j.freeradbiomed.2024.10.312
40	MATSUMOTO Y , NAKASHIMA T , CHO O , et al. Pyruvate-triggered TCA cycle regulation in Staphylococcus aureus promotes tolerance to betamethasone valerate[J]. Biochem Biophys Res Commun, 2020, 528 (2): 318- 321. doi: 10.1016/j.bbrc.2020.05.035
41	YIEW N , FINCK B N . The mitochondrial pyruvate carrier at the crossroads of intermediary metabolism[J]. Am J Physiol Endocrinol Metab, 2022, 323 (1): E33- E52. doi: 10.1152/ajpendo.00074.2022
42	GONZALES-FIGUEROA H , GONZALES-MOLFINO H M . Maturation of pig oocytes in vitro in a medium with pyruvate[J]. Braz J Med Biol Res, 2005, 38 (6): 869- 872. doi: 10.1590/S0100-879X2005000600008
43	JOHNSON M T , FREEMAN E A , GARDNER D K , et al. Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo[J]. Biol Reprod, 2007, 77 (1): 2- 8. doi: 10.1095/biolreprod.106.059899

引物名称 Primer name	引物序列(5′→3′) Sequence	产物长度/bp PCR size	退火温度/℃ Annealing temperature
GAPDH-Forward	CACCCTCAAGATTGTCAGCA	103	58
GAPDH-Reverse	GGTCATAAGTCCCTCCACGA
PKM-Forward	CCCACAGTCGGGATAACCTTG	127	58
PKM-Reverse	TCCAGAGGAACTCCGACG
IDH3α-Forward	TGCAATCTTCGAGTCGGTTCA	130	60
IDH3α-Reverse	CTTCGCAGCGTGGTCAAAAA
β-actin-Forward	GCCCTGAGGCTCTCTTCCA	101	60
β-actin-Reverse	GCGGATGTCGACGTCACA
NDUFA4L2-Forward	CGATGATCGGCTTCATTGGC	103	59
NDUFA4L2-Reverse	GGGTTGTTCTTTCGGTCCCA

组别 Group	体外培养总数/枚 Total number of embryos in vitro	卵裂数/枚(%±SEM) Cleavage number	囊胚数/枚(%±SEM) Number of blastocysts
一二级COCs First- and second-grade COCs	365	285(78.09±3.01)^a	93(32.56±4.33)^a
三四级COCs Third- and fourth-grade COCs	317	192(60.59±6.54)^b	43(22.11±2.79)^b

样本 Sample	Raw reads数量 Raw reads number	Clean reads数量 Clean reads number	Effective reads比率/% Effective reads rate	Mapped reads比率/% Mapped reads rate	Q30值/% Q30 value
GV1-1	45 674 378	43 982 102	96.29	94.42	95.75
GV1-2	51 129 184	49 398 606	96.62	94.28	95.90
GV1-3	54 554 672	52 536 610	96.30	93.81	95.99
GV3-1	67 726 306	65 549 142	96.79	94.24	95.85
GV3-2	48 507 034	46 525 878	95.92	93.56	95.79
GV3-3	46 170 534	44 167 230	95.66	93.49	95.50
MII1-1	54 309 472	53 026 116	97.64	95.64	96.20
MII1-2	52 572 712	50 965 326	96.94	94.99	95.96
MII1-3	58 974 948	57 600 648	97.67	95.27	96.35
MII3-1	61 658 056	59 849 842	97.07	95.19	96.09
MII3-2	65 222 820	62 237 638	95.42	93.64	95.80
MII3-3	69 933 254	67 284 420	96.21	93.76	95.96

组别 Group	培养COCs总数/枚 Total number of COCs cultivated	卵裂数/枚 ((mean±SEM)%) Cleavage number	囊胚数/枚 ((mean±SEM)%) Number of blastocysts
一二级COCs First- and second-grade COCs	412	313 (75.15±4.46)	105 (33.02±2.76)
三四级COCs+丙酮酸钠 Third- and fourth-grade COCs +sodium pyruvate	322	234 (72.54±4.79)^a	71 (30.63±4.15)^a
三四级COCs Third- and fourth-grade COCs	311	192 (61.71±4.75)^b	44 (22.72±2.39)^b

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不同等级牛卵丘-卵母细胞复合体体外成熟后卵母细胞发育能力差异的机制分析

Mechanisms of Developmental Competence Differences of Oocytes from Different Grades of Bovine Cumulus-Oocyte Complexes after in Vitro Maturation

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