滩羊超数排卵处理后血浆类固醇激素对超排效果的影响

doi:10.11843/j.issn.0366-6964.2025.09.024

摘要/Abstract

摘要：

旨在探究超数排卵处理后滩羊血浆类固醇激素变化与超排效果之间的关系。本研究对33只经产年龄在4.5±0.1岁的健康滩羊采用CIDR+FSH+PG法超数排卵，采集试验滩羊在人工授精(AI)当天(第0天)和采胚日(第6天)的血液样品并分离血浆，依据采胚日观察到卵巢黄体数量结合样品采集时间将血浆样品为4组，AI当天高反应组(HCA)、AI当天低反应组(LCA)、采卵日高反应组(HC6)、采卵日低反应组(LC6)，每组各8个样品。采用液相色谱串联质谱(LC-MS/MS)方法，靶向检测血浆中类固醇激素的变化。结果显示，采卵日滩羊血浆中孕酮(P4)、孕烷二醇(PdG)、去氧皮质酮(DOC)和2-甲氧基雌酮(2-ME)极显著高于AI当天，而AI当天(LCA和HCA组)血浆中4-甲氧基雌酮(4-ME)极显著的高于采卵日；对于AI当天，HCA组17α-羟孕酮浓度(FC=1.50)显著高于LCA组(P < 0.05)；采卵日两组中，HC6组孕酮(FC=0.52)和去氧皮质酮(FC=0.50)浓度显著高于LC6组(P < 0.05)，且孕酮和去氧皮质酮浓度与黄体数和总胚胎数之间呈显著强的正相关。结果表明，超数排卵处理后，滩羊血浆类固醇激素发生了变化，采卵日孕酮与去氧皮质酮浓度影响超数排卵效果。

关键词: 滩羊, 超数排卵, 黄体数, 类固醇激素

Abstract:

This study aimed to explore the relationship between changes in plasma steroid hormones and superovulation effects in Tan sheep after superovulation treatment. Thirty-three healthy multiparous Tan sheep aged 4.5±0.1 years were subjected to superovulation using the CIDR+FSH+PG method. Blood samples were collected on the day of artificial insemination (AI, Day 0) and embryo collection (Day 6), and plasma was separated. Plasma samples were divided into 4 groups based on the number of ovarian corpora lutea observed on the embryo collection day and the sampling time: high-response group on AI day (HCA), low-response group on AI day (LCA), high-response group on embryo collection day (HC6), and low-response group on embryo collection day (LC6), with 8 samples in each group. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to target and detect changes in plasma steroid hormones. The results showed that progesterone (P4), pregnanediol (PdG), deoxycorticosterone (DOC) and 2-methoxyestrone) (2-ME) in plasma of Tan sheep on embryo collection day were significantly higher than those on the day of AI, while 4-methoxyestrone (4-ME) in plasma on the day of AI (LCA and HCA groups) was significantly higher than that on the day of egg collection; The concentration of 17α-hydroxyprogesterone in the HCA group (FC=1.50) was significantly higher than that in the LCA group (P < 0.05). The concentrations of progesterone (FC=0.52) and deoxycorticosterone (FC=0.50) in the HC6 group were significantly higher than those in the LC6 group (P < 0.05). Additionally, the concentrations of progesterone and deoxycorticosterone on the embryo collection day (6 d) showed a strong correlation with the number of corpora lutea and the total number of embryos. The results indicate that after superovulation treatment, plasma steroid hormones in Tan sheep change, and the concentrations of progesterone and deoxycorticosterone affect the superovulation effect on the embryo collection day.

Key words: Tan sheep, superovulation, corpus luteum number, steroid hormone

中图分类号:

S826.3

邢洲, 宋承磊, 曹凤凤, 李卓颖, 李庆云, 陶金忠. 滩羊超数排卵处理后血浆类固醇激素对超排效果的影响[J]. 畜牧兽医学报, 2025, 56(9): 4422-4431.

XING Zhou, SONG Chenglei, CAO Fengfeng, LI Zhuoying, LI Qingyun, TAO Jinzhong*. Effects of Plasma Steroid Hormones on Superovulation Efficiency in Tan Sheep after Superovulation Treatment[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4422-4431.

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时期Period	AI后6 d(AI-6d) 6 d after AI (AI-6d)
黄体数分组Corpus luteum number grouping	高反应组	低反应组
采样时间Sample time	HCA(AI时第0天)(n=8)	LCA(AI时第0天)(n=8)
采样时间Sample time	HC6(AI后第6天)(n=8)	LC6(AI后第6天)(n=8)
黄体数Corpus luteum number	17.13±5.46^***	8.00±1.07
胚胎数Number of embryos	9.63±3.02 (P=0.061)	6.25±3.58

差异代谢物 Differential metabolites	KEGG通路注释 KEGG annotation	功能研究 Functional study	参考文献 Reference
17β-羟孕酮 17β-OHP	C01176 map00140	参与糖皮质激素和生殖相关类固醇激素的合成 Involved in the synthesis of glucocorticoids and reproductive steroid hormones	[12]
		维持妊娠，治疗先兆流产，降低早产风险 Maintain pregnancy, treat threatened abortion and reduce the risk of premature birth	[13]
		参与合成盐皮质激素 Participate in the synthesis of mineralocorticoids	[14]
孕酮 P4	C00410 Map00140	促进子宫内膜增厚，促进受精卵着床 Promote endometrial thickening and fertilized egg implantation	[15]
		抑制子宫收缩，保护胎儿，维持妊娠 Inhibit uterine contraction, protect fetus and maintain pregnancy	[16]
		抑制免疫排斥反应，防止胎儿被母体排出 Inhibit immune rejection and prevent the fetus from being discharged by the mother	[17]
去氧皮质酮 DOC	C03205	调节水盐代谢 Regulating water and salt metabolism	[18]
		参与雄烯二酮、睾酮和雄激素的合成 Involved in the synthesis of androstenedione, testosterone and androgen	[19]
		用于诊断糖皮质激素反应性醛固酮增多症 Diagnosis of glucocorticoid reactive aldosteronism	[20]

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