北京黑猪AQP9和RPS10基因多态性及其与背膘厚的关联分析

doi:10.11843/j.issn.0366-6964.2024.01.010

摘要/Abstract

摘要： 旨在研究水通道蛋白9（aquaporins9， AQP9）及核糖体蛋白10（ribosomal protein S10， RPS10）基因多态性及其与北京黑猪背膘厚的相关性，以期能够获得北京黑猪背膘厚的有效标记。本研究以413头北京黑猪为研究对象，测定不同部位（肩部、6-7肋间、胸腰结合、腰荐结合）的背膘厚度。PCR扩增AQP9和RPS10基因的启动子区和外显子区序列筛选SNP位点，并将SNPs与各部位背膘厚进行关联分析。运用荧光定量PCR检测基因的差异表达。结果发现，AQP9基因启动子区有4个突变位点，其中1个SNP：rs332699245 A>C与胸腰结合处背膘厚关联显著（P<0.05），且该位点突变预测到了结合转录因子的改变；RPS10基因在3'UTR、CDS区共有8个突变位点，其中5个与背膘厚显著关联：rs80795904 C>G、rs80932213 T>C与6~7肋间背膘厚关联显著（P<0.05），rs3469834461 C>T与肩部背膘厚、四点平均背膘厚关联显著（P<0.05），rs80862457 C>T与胸腰结合处背膘厚、腰荐结合处背膘厚关联显著（P<0.05），rs336938272 A>C与6~7肋间背膘厚、腰荐结合处背膘厚、四点平均背膘厚均关联显著（P<0.05）；且在3'UTR区检测到1个SNP（rs80862457 C>T），位于小RNA（microRNA，miRNA）结合靶点。基因表达差异分析表明，rs332699245 A>C中AA型个体的AQP9基因mRNA水平显著高于AC型个体（P<0.05），rs80862457 C>T中TT型个体的RPS10基因mRNA水平显著高于CC型个体（P<0.05）。综上所述，AQP9和RPS10基因与北京黑猪背膘厚显著关联，rs332699245 A>C和rs80862457 C>T位点可作为北京黑猪背膘厚选育的潜在分子标记，为北京黑猪的选育提供理论支撑。

关键词: 北京黑猪, AQP9, RPS10, 背膘厚度, 关联分析

Abstract: This study aimed to explore the gene polymorphisms of aquaporins9 (AQP9) and ribosomal protein S10 (RPS10) and their correlation with backfat thickness in Beijing black pigs, in order to obtain effective markers of backfat thickness in Beijing black pigs. The backfat thickness of 413 Beijing black pigs was measured at different parts (shoulder, 6-7 intercostal, thoracolumbar, lumbosacral). The promoter and exon sequences of AQP9 and RPS10 genes were amplified by PCR to screen SNP sites, and the association between SNPs and backfat thickness was analyzed. Differential gene expression was detected by fluorescence quantitative PCR. The results showed that there were 4 mutation sites in the promoter region of AQP9 gene, among which one SNP,rs332699245 A>C, was significantly associated with backfat thickness at the thoracolumbar (P<0.05), and the mutation of this site predicted the change of binding transcription factors. There were 8 mutation sites of RPS10 gene in 3'UTR and CDS region, 5 of which were significantly associated with backfat thickness: rs80795904 C>G and rs80932213 T>C were significantly correlated with 6-7 intercostal backfat thickness (P<0.05), rs3469834461 C>T were significantly correlated with shoulders backfat thickness and 4 regions average backfat thickness (P<0.05),rs80862457 C>T was significantly associated with backfat thickness at the thoracolumbar and lumbosacral (P<0.05), and rs336938272 A>C was significantly associated with 6-7 intercostal backfat thickness, backfat thickness at lumbosacral and 4 regions average backfat thickness (P<0.05). One SNP (rs80862457 C>T) was detected in the 3'UTR region, which was located at the binding target of small RNA (miRNA). Analysis of gene expression differences showed that the AQP9 mRNA expression of AA type individuals in rs332699245 A>C was significantly higher than that of AC type individuals (P<0.05), and the RPS10 mRNA expression of TT type individuals in rs80862457 C>T was significantly higher than that of CC type individuals (P<0.05). In summary, AQP9 and RPS10 genes were significantly associated with backfat thickness of Beijing black pigs, and rs332699245 A>C and rs80862457 C>T sites can be used as potential molecular markers for backfat thickness breeding of Beijing black pigs, providing theoretical support for breeding of Beijing black pigs.

Key words: Beijing black pigs, AQP9, RPS10, backfat thickness, association analysis

中图分类号:

S828.2

祝雪丽, 张龙超, 王立贤, 蒲蕾, 刘欣. 北京黑猪AQP9和RPS10基因多态性及其与背膘厚的关联分析[J]. 畜牧兽医学报, 2024, 55(1): 87-98.

ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.

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