北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析

doi:10.11843/j.issn.0366-6964.2024.01.009

摘要/Abstract

摘要： 旨在开展GREB1L和MIB1基因多态性与肋骨数和胴体性状（胴体长、胴体直长、胴体斜长及胴体重）关联分析，筛选关键功能位点。本研究选取屠宰日龄为215天的317头健康北京黑猪，并提取耳组织DNA，对GREB1L和MIB1基因进行引物设计、并利用聚合酶链式反应（PCR）技术和Sanger测序技术等对SNPs进行基因分型，计算变异位点基因型频率以及等位基因频率分布，并与肋骨数和胴体性状进行关联分析。试验共筛选到6个SNPs，包括GREB1L基因的3个同义突变、MIB1基因的2个同义突变和1个内含子区的可变剪切突变。将6个SNPs分别对肋骨数和胴体性状使用Duncan’s多重检验统计分析发现，GREB1L基因上的3个同义突变6：106574972 G>A、6：106648540 C>T、6：106648558 A>G与性状关联均不显著（P>0.05）；MIB1基因上的1个同义突变6：106936590 C>T与胴体长显著相关（P<0.05）；而内含子15~16的6：107016475 A>G可变剪切突变与肋骨数显著相关（P<0.05）；第三个同义突变6：107028660 G>A与肋骨数和胴体性状均无显著差异（P>0.05）。结果显示，显著的2个位点可作为北京黑猪肋骨数及胴体长变异的候选基因功能位点。

关键词: 北京黑猪, GREB1L, MIB1, 肋骨数, 胴体性状

Abstract: The study aimed to conduct an association analysis of GREB1L and MIB1 gene polymorphisms with rib number and carcass traits (carcass length, straight length, diagonal length and carcass weight), in order to screen for key functional loci. In this study, DNA was extracted from ear tissue of 317 healthy Beijing black pigs at the age of 215 days. The primers were designed for GREB1L and MIB1. The exon regions of two genes were genotyped by PCR and Sanger sequencing. The distribution of genotype frequency and allele frequency of the mutated loci was calculated, and their association analysis with rib number and carcass traits was performed. A total of 6 SNPs were detected in two genes, including 3 synonymous mutations in GREB1L, 2 synonymous mutations in MIB1, one splice donor 5th base variant-intron variant in MIB1. Statistical analysis using Duncan’s multiple test revealed that 3 synonymous mutations (6:106574972 G>A, 6:106648540 C>T, 6:106648558 A>G) in GREB1L and one synonymous mutation (6:107028660 G>A) in MIB1 were no significant association with traits (P>0.05). A synonymous mutation in MIB1 (6:106936590 C>T) was significant association with carcass length and one splice donor 5th base variant-intron variant in MIB1 (6:107016475 A>G) was significant association with the number of rib (P<0.05). In conclusion, the above two significant SNPs could be used as candidate functional loci for the variation of rib number and carcass length of Beijing black pigs.

Key words: Beijing black pigs, GREB1L, MIB1, rib number, carcass traits

中图分类号:

S828.2

牛乃琪, 赵润泽, 宗文成, 刘先策, 刘海, 石国华, 井西涛, 张龙超. 北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析[J]. 畜牧兽医学报, 2024, 55(1): 79-86.

NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.

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