白头翁散煎剂发酵物对感染猪流行性腹泻病毒仔猪肠道屏障功能的影响

doi:10.11843/j.issn.0366-6964.2023.10.036

摘要/Abstract

摘要： 试验旨在研究白头翁散对感染猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）仔猪肠道屏障功能的影响。将24头7日龄体重相近的仔猪（杜×长×大）随机分为4组，分别为对照组、PEDV组、煎剂组和发酵组。试验期为12 d，第4天，对照组仔猪接种生理盐水，其他3组仔猪每头以10^4.5 TCID₅₀（50%组织培养感染剂量）的剂量接种PEDV （HN2021），第5~9天，煎剂组和发酵组仔猪每天灌服煎剂和发酵液（0.2 mL·kg^-1）。评估仔猪的生长性能、腹泻指数、病毒拷贝量、肠道细菌组成、肠道形态、肠道屏障功能，以及血清和肠道中的抗氧化指标。结果表明：煎剂和发酵液可显著提高PEDV感染仔猪平均日增重（average daily gain，ADG），降低腹泻率，抑制肠道内病毒复制（P<0.05），还可调节肠道细菌组成，显著提高乳酸杆菌和双歧杆菌数量（P<0.05）；给药缓解了PEDV感染造成的仔猪肠道绒毛高度（villus height，VH）显著下降和隐窝深度（crypt depth，CD）增加，改善肠道形态，显著提高血清中D-木糖含量，降低肠型脂肪酸结合蛋白（I-FABP）浓度，从而增强肠道屏障功能（P<0.05）。此外，煎剂和发酵液可降低氧化应激，表现为总超氧化物歧化酶（T-SOD）活性显著升高，丙二醛（MDA）含量显著降低（P<0.05）。这些数据表明白头翁散可增强感染PEDV仔猪的肠道屏障功能。

关键词: 白头翁散, 发酵, PEDV, 生长性能, 肠道屏障功能, 抗氧化功能

Abstract: The trial aimed to study the effect of Pulsatilla Powder prescription on the intestinal barrier function of piglets infected with porcine epidemic diarrhea virus (PEDV). Twenty-four 7-day-old piglets (du×long×large) were randomly divided into 4 groups:control, PEDV, decoction and fermentation groups. The test period was 12 days. On day 4, piglets in the control group were inoculated with saline and the other three groups were inoculated with PEDV (HN2021) at a dose of 10^4.5 TCID₅₀ (50% tissue culture infection dose) per pig, and on days 5-9, the decoction and fermentation groups were orally given decoction and fermentation solution (0.2 mL·kg^-1) daily. To evaluate the growth performance, diarrhea index, viral copies, intestinal bacterial composition, intestinal morphology, intestinal barrier function of piglets, and antioxidant indicators in the serum and intestine. The results showed that the decoction and fermentation solution significantly increased the average daily gain (ADG), reduced diarrhea rate and inhibited viral replication in the intestine of the PEDV-infected piglets (P<0.05); and also regulated the intestinal bacteria composition of piglets, significantly increased the number of Lactobacillus and Bifidobacterium(P<0.05); the administration alleviated the significant decrease in intestinal villus height (VH) and increase in crypt depth (CD) in piglets caused by PEDV infection, improved intestinal morphology, significantly increased D-xylose content and decreased fatty acid binding protein (I-FABP) concentration in serum, thereby enhancing intestinal barrier function (P<0.05). In addition, the decoction and fermentation solution reduced oxidative stress, manifested by significantly increased total superoxide dismutase (T-SOD) activity and decreased malondialdehyde (MDA) content (P<0.05). These dates indicate Pulsatilla Powder can enhance intestinal barrier function in PEDV-infected piglets.

Key words: Pulsatilla Powder, fermentation, PEDV, growth performance, intestinal barrier function, antioxidant function

中图分类号:

S853.9

常伟辰, 李帅奇, 李琰, 闫微, 张红英, 王彦彬, 杨明凡, 张昂克. 白头翁散煎剂发酵物对感染猪流行性腹泻病毒仔猪肠道屏障功能的影响[J]. 畜牧兽医学报, 2023, 54(10): 4403-4410.

CHANG Weichen, LI Shuaiqi, LI Yan, YAN Wei, ZHANG Hongying, WANG Yanbin, YANG Mingfan, ZHANG Angke. Effect of Pulsatilla Powder Prescription Decoction Ferments on Intestinal Barrier Function of Piglets Infected with Porcine Epidemic Diarrhea Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4403-4410.

参考文献 19

[1]	朱向东, 高冉. 猪流行性腹泻的研究进展[J]. 猪业科学, 2022, 39(7):100-101.ZHU X D, GAO R. Research progress on epidemic diarrhea in pigs[J]. Swine Industry Science, 2022, 39(7):100-101. (in Chinese)
[2]	董世娟, 朱于敏, 于瑞嵩, 等. 我国PEDV分子流行病学研究进展[C]//中国畜牧兽医学会家畜传染病学分会第八届全国会员代表大会暨第十五次学术研讨会论文集. 徐州:中国畜牧兽医学会, 2013.DONG S J, ZHU Y M, YU R S, et al. Research progress in molecular epidemiology of PEDV in China[C]//The 8th National Congress of the Chinese Society of Animal Husbandry and Veterinary Medicine and the 15th Symposium on Infectious Diseases of Domestic Animals. Xuzhou:Chinese Society of Animal Husbandry and Veterinary Medicine, 2013. (in Chinese)
[3]	ZHONG J M, TAN L H, CHEN M W, et al. Pharmacological activities and molecular mechanisms of Pulsatilla saponins[J]. Chin Med, 2022, 17(1):59.
[4]	LIU Y L, ZHOU M Y, YANG M, et al. Pulsatilla chinensis saponins ameliorate inflammation and DSS-induced ulcerative colitis in rats by regulating the composition and diversity of intestinal flora[J]. Front Cell Infect Microbiol, 2021, 11:728929.
[5]	HE J, YUAN R Y K, CUI X L, et al. Anemoside B4 protects against Klebsiella pneumoniae-and influenza virus FM1-induced pneumonia via the TLR4/Myd88 signaling pathway in mice[J]. Chin Med, 2020, 15:68.
[6]	苗艳, 朱庆贺, 兰世捷, 等. 中药益生菌发酵在兽医临床中的研究进展[J]. 现代畜牧科技, 2021(9):14-17, 22.MIAO Y, ZHU Q H, LAN S J, et al. Research progress of probiotics fermentation in veterinary clinic[J]. Modern Animal Husbandry Science & Technology, 2021(9):14-17, 22. (in Chinese)
[7]	GUO X, YAN Z P, WANG J X, et al. Effect of traditional chinese medicine (TCM) and its fermentation using Lactobacillus plantarum on ceftriaxone sodium-induced dysbacteriotic diarrhea in mice[J]. Chin Med, 2022, 17(1):20.
[8]	HUANG P, WANG P, XU J X, et al. Fermented traditional Chinese medicine alters the intestinal microbiota composition of broiler chickens[J]. Res Vet Sci, 2021, 135:8-14.
[9]	马步仓, 赵海霞, 韩莉萍, 等. 益生菌及其在畜禽生产中的应用[J]. 中国饲料, 2023(1):66-71.MA B C, ZHAO H X, HAN L P, et al. Probiotics and their application in livestock and poultry production[J]. China Feed, 2023(1):66-71. (in Chinese)
[10]	ZHANG J M, ZHAO D, YI D, et al. Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus[J]. Sci Rep, 2019, 9(1):19798.
[11]	王靓靓, 李训良, 李鹏冲, 等. 猪流行性腹泻的诊断与预防[J]. 世界华人消化杂志, 2013, 21(1):33-38.WANG L L, LI X L, LI P C, et al. Diagnosis and prevention of porcine epidemic diarrhea[J]. World Chinese Journal of Digestology, 2013, 21(1):33-38. (in Chinese)
[12]	OH Y R, LEE J B, PARK S Y, et al. In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets[J]. Korean J Vet Res, 2008, 48(2):167-174.
[13]	JUNG K, WANG Q H, SCHEUER K A, et al. Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs[J]. Emerg Infect Dis, 2014, 20(4):662-665.
[14]	YANG S S, LI Y, WANG B, et al. Acute porcine epidemic diarrhea virus infection reshapes the intestinal microbiota[J]. Virology, 2020, 548:200-212.
[15]	BIAN X, WANG T T, XU M, et al. Effect of lactobacillus strains on intestinal microflora and mucosa immunity in Escherichia coli O157:H7-induced diarrhea in mice[J]. Curr Microbiol, 2016, 73(1):65-70.
[16]	XIN J G, ZENG D, WANG H S, et al. Probiotic Lactobacillus johnsonii BS15 promotes growth performance, intestinal immunity, and gut microbiota in piglets[J]. Probiotics Antimicrob Proteins, 2020, 12(1):184-193.
[17]	CAO Y N, ZHANG S S, HUANG Y J, et al. The aqueous leaf extract of M. oleifera inhibits PEDV replication through suppressing oxidative stress-mediated apoptosis[J]. Animals, 2022, 12(4):458.
[18]	WANG L, ZHOU J, HOU Y Q, et al. N-Acetylcysteine supplementation alleviates intestinal injury in piglets infected by porcine epidemic diarrhea virus[J]. Amino Acids, 2017, 49(12):1931-1943.
[19]	JUNG K, EYERLY B, ANNAMALAI T, et al. Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus[J]. Vet Microbiol, 2015, 177(3-4):373-378.