基于网络药理学分析蒲公英抗氧化功能的物质基础与作用机制

doi:10.11843/j.issn.0366-6964.2023.05.037

摘要/Abstract

摘要： 本研究旨在基于网络药理学研究蒲公英抗氧化功能的物质基础和潜在作用机制,并对以蒲公英为原料开发功能性植物提取物饲料添加剂提供指导。通过HERB本草组鉴数据库、TCMSP数据库和SwissTargetPrediction网页工具筛选蒲公英中的活性成分以及潜在作用靶点,使用SwissADME工具计算活性成分的理化性质,从GeneCards数据库中获得与抗氧化相关的目的基因,使用Cytoscape和STRING数据库构建化合物-靶点-功能、化合物-靶点-通路可视化网络和蛋白质-蛋白质相互作用(PPI)网络,通过DAVID数据库进行基因本体论(GO)、京都基因和基因组百科全书(KEGG)途径富集分析,通过ABTS法和FRAP法对蒲公英不同提取组分进行了体外抗氧化活性测定。结果显示:筛选到活性成分28个、化合物预测靶点296个、抗氧化靶点1 371个和交集靶点135个。理化性质计算显示,活性成分中大部分为水溶性。蛋白质互作分析表明,JUN、VEGFA、SRC、HSP90AA1和MMP9等20个关键蛋白可能在抗氧化功能中发挥关键作用。GO和KEGG富集分析表明,抗氧化功能可能与血管内皮生长因子通路、TNF信号通路、MAPK信号通路、IL-17信号通路和雌激素信号通路有关。体外抗氧化测定结果显示,蒲公英水提组分具有更高的抗氧化活性。综上表明,蒲公英中的水溶性活性成分是其抗氧化功能的主要物质基础,为进一步开发抗氧化功能的蒲公英植物提取物饲料添加剂提供理论支持。

关键词: 蒲公英, 网络药理学, 抗氧化, 物质基础, 植物提取物饲料添加剂

Abstract: The aim of this study was to investigate the material basis and potential mechanism of action of the antioxidant function of Taraxacum mongolicum (T. mongolicum) based on network pharmacology and to provide guidance for the development of T. mongolicum as a functional plant extracts feed additive. The active ingredients and potential targets in T. mongolicum were screened by HERB database, TCMSP database and SwissTargetPrediction web tool, and the physicochemical properties of the active ingredients were calculated using SwissADME tool, and the target genes related to antioxidant function were obtained from GeneCards database. Compound-target-function, compound-target-pathway visualization networks and protein-protein interaction (PPI) networks were constructed using Cytoscape and STRING databases. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed by DAVID database. In vitro antioxidant activities of different extract fractions of T. mongolicum were determined by ABTS and FRAP assays. The screening yielded 28 active ingredients, 296 compound prediction targets, 1 371 antioxidant targets and 135 intersection targets. Physicochemical calculations showed that most of the active ingredients were water soluble. Protein-protein interaction analysis indicated that 20 key proteins, including JUN, VEGFA, SRC, HSP90AA1 and MMP9, may play key roles in antioxidant function. GO and KEGG enrichment analysis indicated that antioxidant function may be related to vascular endothelial growth factor pathway, TNF signaling pathway, MAPK signaling pathway, IL-17 signaling pathway and estrogen signaling pathway. In vitro antioxidant tests showed higher antioxidant activity of aqueous extracts in T. mongolicum. The results suggest that the water-soluble active ingredients in T. mongolicum are the main material basis for its antioxidant function, providing theoretical support for further development of T. mongolicum as an antioxidant functional feed additive.

Key words: Taraxacum mongolicum, network pharmacology, antioxidant, material basis, plant extracts feed additive

中图分类号:

杨子辉, 董朕, 伍蕙岚, 谭斌, 曾建国. 基于网络药理学分析蒲公英抗氧化功能的物质基础与作用机制[J]. 畜牧兽医学报, 2023, 54(5): 2170-2185.

YANG Zihui, DONG Zhen, WU Huilan, TAN Bin, ZENG Jianguo. Analysis of the Material Basis and Mechanism of Action of Antioxidant Function of Taraxacum mongolicum based on Network Pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2170-2185.

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