绵羊生长性状间的相关和回归分析及TRHDE基因多态性与生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2023.04.007

摘要/Abstract

摘要： 旨在探究乌珠穆沁羊生长性状间的相关性，构建体尺对体重的最优回归方程，分析TRHDE基因多态性对乌珠穆沁羊生长性状的影响。本研究选取336只生长发育良好的乌珠穆沁羊（公羊176只、母羊160只）作为研究对象，收集4月龄和6月龄生长性状，利用绵羊Illumina OvineSNP600 BeadChip在336只乌珠穆沁羊群体中对TRHDE基因SNPs进行基因型检测，采用SPSS 26.0软件对生长性状的相关性和回归性进行分析以及TRHDE基因多态性与生长性状进行关联分析。结果发现，乌珠穆沁羊4月龄和6月龄的公羊和母羊生长性状最优回归方程分别为：Y₁=-53.66＋0.77X₁＋0.39X₂、Y₂=-47.83＋0.63X₁＋0.45X₂、Y₃=-47.01＋0.42X₁＋0.13X₂＋2.82X₃＋0.19X₄＋0.28X₅、Y₄=-61.32＋0.25X₁＋0.40X₂＋3.15X₃＋0.39X₄。TRHDE基因SNPs与乌珠穆沁羊生长性状关联分析结果显示，rs404402551A>G位点与4月龄体重和胸围、6月龄体重、胸围和胸宽呈显著或极显著关联（P<0.05；P<0.01）；rs415608385C>A位点与4月龄体重、6月龄体重、体高、体长和胸宽均呈显著或极显著关联（P<0.05；P<0.01）；rs426657887G>A位点与乌珠穆沁羊生长性状无显著关联（P>0.05）；rs404402551A>G和rs426657887G>A位点在乌珠穆沁羊中处于强连锁不平衡。综上所述，乌珠穆沁羊体重的选择潜力较大，可将胸围作为评估乌珠穆沁羊体重的重要指标；TRHDE基因的rs404402551A>G和rs415608385C>A位点可作为乌珠穆沁羊生长发育的潜在分子标记，为乌珠穆沁羊分子标记辅助选择育种提供参考。

关键词: 乌珠穆沁羊, TRHDE基因, 生长性状, 相关分析, 回归分析, 关联分析

Abstract: This study aimed to explore the correlation among growth traits in Ujumqin sheep and the optimal regression equation of body size to body weight was established, and analyze the effect of TRHDE gene polymorphism on the growth traits of Ujumqin sheep. In this study, 336 well-developed Ujumqin sheep (176 males and 160 females) were selected as research objects, and growth traits were collected at 4-month-old and 6-month-old. Genotypes of SNPs in the TRHDE gene were detected using the sheep Illumina OvineSNP600 BeadChip in a population of 336 Ujumqin sheep. SPSS 26.0 software was used for correlation and regression analysis among growth traits and association analysis between TRHDE gene polymorphism and growth traits. The results showed that the optimal regression equations of 4-month-old and 6-month-old male and female Ujumqin sheep were as follows:Y₁=-53.66+0.77X₁+0.39X₂, Y₂=-47.83+0.63X₁+0.45X₂, Y₃=-47.01+0.42X₁+0.13X₂+2.82X₃+0.19X₄+0.28X₅, Y₄=-61.32+0.25X₁+0.40X₂+3.15X₃+0.39X₄. The results of the association of TRHDE gene SNPs with growth traits in Ujumqin sheep showed that rs404402551A>G locus was significantly or extremely significantly associated with body weight and chest circumference at 4-month-old and body weight, chest circumference and chest width at 6-month-old (P<0.05; P<0.01). The rs415608385C>A locus was significantly or extremely significantly associated with body weight at 4-month-old and body weight, body height, body length and chest width at 6-month-old (P<0.05; P<0.01). The rs426657887G>A locus was not significantly associated with each growth trait in Ujumqin sheep (P>0.05). And the rs404402551A>G and rs426657887G>A loci were in strong linkage disequilibrium in the Ujumqin sheep. In conclusion, the greater potential for selection of body weight in Ujumqin sheep and chest circumference can be used as an important indicator for assessing body weight in Ujumqin sheep. The rs404402551A>G and rs415608385C>A loci of the TRHDE gene can be used as potential molecular markers for growth and development in Ujumqin sheep, which can provide reference for molecular marker-assisted selection for breeding in Ujumqin sheep.

Key words: Ujumqin sheep, TRHDE gene, growth traits, correlation analysis, regression analysis, association analysis

中图分类号:

S826.2

毕亚珍, 尚明玉, 胡文萍, 张莉. 绵羊生长性状间的相关和回归分析及TRHDE基因多态性与生长性状的关联分析[J]. 畜牧兽医学报, 2023, 54(4): 1415-1428.

BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.

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