北京黑猪DKK3和CCR1基因多态性检测及其与背膘厚的关联分析

doi:10.11843/j.issn.0366-6964.2022.07.006

摘要/Abstract

摘要： 旨在探讨DKK3和CCR1基因多态性及其对北京黑猪背膘厚的影响，以期能够筛选出可用于提高北京黑猪背膘厚性状的分子标记，为北京黑猪优良性状定向选育提供依据。本研究以413头健康的北京黑猪为试验材料，对其进行DNA提取、PCR扩增、基因测序筛选候选基因的SNPs，同时利用SAS 9.2软件对突变位点的不同基因型分别与北京黑猪的六七肋背膘厚和腰荐部背膘厚进行关联分析。结果显示，在北京黑猪DKK3基因的外显子区共检测到10个SNPs，包含1个可变剪切突变、1个错义突变和8个3'UTR突变，其中有4个3'UTR突变位点均与腰荐部背膘厚显著关联(P<0.05)，分别为g.47443779 T>C、g.47444258 C>T、g.47444912 G>T和g.47445304 T>C，其余位点与六七肋背膘厚和腰荐部背膘厚均不显著关联(P>0.05)。在CCR1基因的外显子区共检测到3个SNPs，包含2个同义突变和1个错义突变，其中1个同义突变位点g.29229037 G>A与六七肋背膘厚显著关联(P<0.05)。对DKK3的10个SNPs进行连锁不平衡分析，结果显示，g.47443779 T>C和g.47443783 C>T处于完全连锁状态(D’=1)，g.47443783 C>T和g.47443858 C>T处于完全连锁状态(D’=1)，g.47444258 C>T和g.47444275 C>T呈现高度连锁状态(D’=0.98)。综上所述，DKK3和CCR1基因的SNPs分别与北京黑猪的腰荐部背膘厚和六七肋背膘厚有显著关联性，可为北京黑猪背膘厚的早期分子选育提供参考。

关键词: 北京黑猪, DKK3, CCR1, 背膘厚

Abstract: The aim of this study was to investigate the polymorphism of DKK3 and CCR1 genes and their effects on backfat thickness of Beijing black pigs, so as to screen out molecular markers that can be used to improve backfat thickness traits and provide basis for directional breeding of excellent traits in Beijing black pigs. DNA extraction, PCR amplification and gene sequencing were performed on 413 healthy Beijing black pigs to screen SNPs of candidate genes. At the same time, SAS 9.2 software was used to analyze the association between different genotypes of mutation sites and backfat thickness of 6-7th ribs, backfat thickness of lumbosacral region. The results showed that a total of 10 SNPs were detected in the exons of DKK3 gene in Beijing black pig, including 1 splice region variant, 1 missense variant and 8 3'UTR variants. Among them, 4 SNPs of 3' UTR variants were significantly associated with the backfat thickness of lumbosacral region (P<0.05), which were g.47443779 T > C, g.47444258 C > T, g.47444912 G > T and g.47445304 T > C, respectively, and other SNPs had no significant correlation with backfat thickness of lumbosacral region or backfat thickness of 6-7th ribs (P>0.05). A total of 3 SNPs were detected in the exon of CCR1 gene, including 2 synonymous variant and 1 missense variant. One synonymous variant (g.29229037 G > A) was significantly associated with the backfat thickness of 6-7th ribs (P<0.05). The linkage disequilibrium analysis of 10 SNPs in DKK3 showed that g. 47443779 T > C and g. 47443783 C > T were in a completely linked state (D'=1), g. 47443783 C > T and g. 47443858 C > T were in a completely linked state (D'=1), g. 47444258 C > T and g. 47444275 C > T were in a highly linked state (D'=0.98). In summary, SNPs of DKK3 and CCR1 genes were respectively significantly associated with the backfat thickness of lumbosacral region and backfat thickness of 6-7th ribs of Beijing black pig, which could provide reference for the early molecular breeding of backfat thickness in Beijing black pig.

Key words: Beijing black pigs, DKK3, CCR1, backfat thickness

中图分类号:

S828.2

田威龙, 兰干球, 张龙超, 王立贤, 梁晶, 刘欣. 北京黑猪DKK3和CCR1基因多态性检测及其与背膘厚的关联分析[J]. 畜牧兽医学报, 2022, 53(7): 2083-2093.

TIAN Weilong, LAN Ganqiu, ZHANG Longchao, WANG Lixian, LIANG Jing, LIU Xin. Detection of DKK3 and CCR1 Genes Polymorphisms and Their Association with Backfat Thickness in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2083-2093.

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