Ⅲ型分泌系统2转录因子YqeI对禽致病性大肠杆菌生物被膜调控机制的研究

doi:10.11843/j.issn.0366-6964.2019.12.013

摘要/Abstract

摘要： 生物被膜是导致细菌产生耐药性的主要原因之一，本文通过探究Ⅲ型分泌系统2（ETT2）转录调节因子YqeI对禽致病性大肠杆菌生物被膜形成的影响及其影响机制，为探究ETT2对禽致病性大肠杆菌致病机制的影响提供研究基础。利用Red同源重组的方法构建yqeI基因缺失株，并通过检测野生株与缺失株生物被膜形成能力、结合转录组学测序及荧光定量检测生物被膜基因表达量等，探究转录调节因子YqeI对禽致病性大肠杆菌生物被膜形成能力的影响。结果显示成功构建了yqeI基因缺失株，且yqeI的缺失并不影响生长曲线，但生物被膜形成能力显著下降，且相关生物被膜基因转录量显著下调。禽致病性大肠杆菌ETT2转录调节因子YqeI显著影响了禽致病性大肠杆菌生物被膜形成能力，为从ETT2及yqeI的角度发掘潜在的调控网络提供依据。

关键词: 禽致病性大肠杆菌, Ⅲ型分泌系统2, 转录因子YqeI, 生物被膜

Abstract: Biofilm is one of the main cause of bacterial resistance. In this study, the effects of type Ⅲ secretion system 2 (ETT2) transcription regulator YqeI on the biofilm formation of avian pathogenic Escherichia coli and its influencing mechanism were investigated. Red homologous recombination method was used to construct yqeI gene deletion strains, and the effect of transcription regulator YqeI on the biofilm formation ability of avian pathogenic E. coli was explored by detecting the biofilm formation ability of wild strains and deletion strains, combining transcriptome sequencing and fluorescence quantitative detection of biofilm gene transription. The yqeI gene deletion strain was successfully constructed, and the deletion of yqeI does not affect the growth curve, but the biofilm formation ability was significantly decreased and the transription of the relevant biofilm gene was significantly down-regulated. The avian pathogenic E. coli ETT2 transcriptional regulator YqeI significantly affected the biofilm formation ability of avian pathogenic E.coli, providing a possibility to find new drug targets from the perspective of yqeI and related genes.

Key words: avian pathogenic Escherichia coli, type Ⅲ secretion system 2, transcription factor YqeI, biofilm

中图分类号:

S852.612

肖亚婷, 傅丹丹, MUHAMMAD AKMAL RAHEEM, 薛媚, 谷一, 涂健, 宋祥军, 邵颖, 祁克宗. Ⅲ型分泌系统2转录因子YqeI对禽致病性大肠杆菌生物被膜调控机制的研究[J]. 畜牧兽医学报, 2019, 50(12): 2488-2497.

XIAO Yating, FU Dandan, MUHAMMAD AKMAL RAHEEM, XUE Mei, GU Yi, TU Jian, SONG Xiangjun, SHAO Ying, QI Kezong. Study on the Regulation Mechanism of Type Ⅲ Secretion System 2 Transcription Factor YqeI on the Biofilm of Avian Pathogenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2488-2497.

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