Fur/RyhB调控禽致病性大肠杆菌运动性机制的研究

doi:10.11843/j.issn.0366-6964.2020.02.016

摘要/Abstract

摘要： 大肠杆菌的运动性主要由鞭毛提供动力，鞭毛是致病性大肠杆菌重要毒力因子之一，本文通过探究Fur及其负调控的非编码RNA——RyhB对禽致病性大肠杆菌（APEC）运动性的影响，为探索防控禽大肠杆菌病的潜在靶点提供科学依据。通过Red同源重组方法构建AE17△Fur和AE17△Fur/RyhB，比较缺失株与原始株运动性特征，结合转录组学数据探究Fur和RyhB对APEC鞭毛以及生物被膜形成的影响。结果显示成功构建了AE17△Fur和AE17△Fur/RyhB，转录组学结果显示Fur的缺失基本上使所有鞭毛相关的基因下调。Fur的缺失使APEC的运动性显著减弱，RyhB对APEC的运动性没有显著影响，△Fur/RyhB较△Fur运动能力有所增加。△Fur和△Fur/RyhB生物被膜形成能力显著增强，△RyhB生物被膜形成减弱，与运动性趋势基本一致。Fur对禽致病性大肠杆菌的鞭毛组装有非常重要的作用，其负调控的RyhB一定程度上可以抑制这种作用机制的影响，并进一步影响了生物被膜的形成，为寻找相关药物靶点提供可能。

关键词: 禽致病性大肠杆菌, Fur, RyhB, 运动性, 铁稳态

Abstract: The motility of E. coli is mainly driven by flagella, which is one of the important virulence factors of pathogenic E. coli. In this paper, the influences of Fur and its negatively regulated non-coding RNA-RyhB on the motility of avian pathogenic E. coli (APEC) AE17 strain were investigated to provide scientific basis for exploring potential targets for prevention and control of avian E. coli. The Red homologous recombination method was used to construct AE17△Fur and AE17△Fur/RyhB, then the motility characteristics of the absent and original strains were compared, and the effects of Fur and RyhB on the formation of APEC flagellum and biofilm in combination were explored with transcriptome data. The results showed that the AE17△Fur and AE17△Fur/RyhB were constructed successfully. Transcriptome results showed that the absence of Fur basically down-regulated all flagella-related genes. The absence of Fur significantly weakened the movement of APEC, and RyhB had no significant influence on the movement of APEC. △Fur/RyhB has increased its movement ability compared with △Fur. Biofilm formation ability of △Fur and △Fur/RyhB were significantly enhanced, and that of △RyhB was weakened, which were consistent with the trend of movement. Fur plays a very important role in flagellum assembly of pathogenic E. coli, and its negatively regulated RyhB can inhibit the effect of this mechanism to some extent, and further affect the formation of biofilm, so as to provide a possibility for finding relevant drug targets.

Key words: avian pathogenic Escherichia coli, Fur, RyhB, motility, iron homeostasis

中图分类号:

S852.612

涂健, 蔡伟真, 阮苑, 宋祥军, 邵颖, 祁克宗. Fur/RyhB调控禽致病性大肠杆菌运动性机制的研究[J]. 畜牧兽医学报, 2020, 51(2): 346-355.

TU Jian, CAI Weizhen, RUAN Yuan, SONG Xiangjun, SHAO Ying, QI Kezong. Study on the Regulation of Motility Mechanism by Avian Pathogenic Escherichia coli Fur Combined with RyhB[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 346-355.

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