牛坏死杆菌43K OMP基因缺失株生物学特性分析

doi:10.11843/j.issn.0366-6964.2022.11.027

摘要/Abstract

摘要： 旨在研究牛坏死杆菌（Fusobacterium necrophorum）43K OMP基因的生物学功能。通过对比亲本株（A25株）和43K OMP基因缺失株（A25Δ43K OMP株）的体外生长速率、药物敏感性、生物被膜形成能力、对细胞的黏附能力、毒性以及对Balb/c雌性小鼠致病力，初步研究43K OMP基因在坏死杆菌致病中潜在的生物功能。结果显示，当43K OMP基因被缺陷后，牛坏死杆菌的体外生长速率和对细胞的毒性无显著差异（P>0.05）；对克拉霉素、卡那霉素、新霉素、阿米卡星、奈替米星、呋喃妥因、多黏菌素B、利福平和环丙沙星耐药性减弱；培养72和96 h后，生物被膜形成能力显著增强（P<0.05）；与BEND细胞共孵育1 h后，细菌黏附能力显著降低（P<0.05）；对Balb/c雌性小鼠致病力明显减弱（P<0.05）。因此，43K OMP基因主要与坏死杆菌的黏附能力、生物被膜形成能力和耐药性有关。

关键词: 坏死杆菌, 43K OMP, 细胞黏附, 生物被膜, 耐药性

Abstract: The aim of this study was to investigate the biological function of the 43K OMP gene of Fusobacterium necrophorum. In vitro growth rate, drug sensitivity, biofilm formation ability, bacterial adhesion ability, bacterial toxicity to cells and pathogenicity biological properties to Balb/c female mice between the wild type strain (A25 strain) and 43K OMP gene defective strain (A25Δ43K OMP strain) were detected to investigate the potential biological function of 43K OMP gene in the pathogenesis of Fusobacterium necrophorum. The results showed that when 43K OMP gene was mutated, there was no significant difference in growth rate or bacterial toxicity to cells of F. necrophorum (P>0.05); Resistance to claricid, kanamycin, neomycin, amikacin, netilmicin, furantoin, polymyxin B, rifampicin and ciprofloxacin were diminished; Biofilm formation ability was significantly enhanced after 72 and 96 h of incubation (P<0.05); After co-incubation with BEND cells for 1 h, the bacterial adhesion ability was significantly reduced (P<0.05); The pathogenicity to Balb/c female mice was significantly weakened (P<0.05). Therefore, 43K OMP gene is mainly related to the bacterial adhesion ability, biofilm formation ability and drug resistance of F. necrophorum.

Key words: Fusobacterium necrophorum, 43K OMP, cell adhesion, biofilm, drug resistance

中图分类号:

S852.615

蒋凯, 赵鹏宇, 王天硕, 于思雯, 毕栏, 肖佳薇, 贺显晶, 郭东华. 牛坏死杆菌43K OMP基因缺失株生物学特性分析[J]. 畜牧兽医学报, 2022, 53(11): 4019-4026.

JIANG Kai, ZHAO Pengyu, WANG Tianshuo, YU Siwen, BI Lan, XIAO Jiawei, HE Xianjing, GUO Donghua. Analysis of Biological Characteristics of Fusobacterium necrophorum 43K OMP Genes Mutant Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4019-4026.

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