猪圆环病毒2型感染PK-15细胞差异表达miRNA和mRNA互作网络

doi:10.11843/j.issn.0366-6964.2019.01.014

摘要/Abstract

摘要：

旨在筛选猪圆环病毒2型感染猪肾上皮细胞（PK-15）后差异表达miRNA和mRNA，并探究miRNA和mRNA之间的互作关系。使用茎环定量PCR检测多个热点miRNA在PCV2感染组和未接种病毒组之间的差异表达水平以发现差异表达miRNA，分析PCV2感染后第12小时的蛋白质组学研究结果（GEO登录号：GSE71945）以获得差异表达mRNA，并分析差异表达miRNA和mRNA之间的互相作用关系。结果显示，在PCV2感染后第12小时miR-10b、miR-128、miR-155-5p、miR-21、miR-29b、miR-361-3p等表达量显著性变化，使用miRecords预测其靶基因，分别发现7 105、9 741、7 451、7 183、7 971、8 793个靶基因；PCV2感染后第12小时共有158个差异表达mRNA，分属于多种细胞结构成分并参与多条重要的细胞生物途径，且差异miRNA的靶基因和差异mRNA之间有大量的交集基因。上述结果表明，PCV2感染能导致细胞内多种miRNA差异表达，造成miRNA的靶基因表达紊乱，进而影响细胞正常的生命进程。

Abstract:

The purpose of this study was to elucidate changes in miRNA and mRNA expression profiles of PCV2 infected PK-15 cells, and to illuminate the interaction between differentially expressed miRNAs and mRNAs. Stem-loop quantitative PCR was used to detect and identify differential expression levels of multiple hot spot miRNAs in PCV2 infected and uninfected groups. The results of proteomic study of PCV2 infected cells at the 12th hour post infection (hpi) were analyzed (GEO Accession No.:GSE71945) to obtain differentially expressed mRNA and to analyze the interaction between differentially expressed miRNA and mRNA. It was found that miR-10b, miR-128, miR-155-5p, miR-21, miR-29b, miR-361-3p were differentially expressed at 12 hpi. By using miRecords to predict their target genes, 7 105, 9 741, 7 451, 7 183, 7 971 and 8 793 target genes were found, respectively. Totally there were 158 differentially expressed mRNAs that belonged to various cell components and participated in many important biological pathways, and there was a large number of common target genes between the differentially expressed miRNAs candidate target genes and differentially expressed mRNAs. In summary, PCV2 infection can lead to disruption of multiple miRNA expression in cells, resulting in disorganization of miRNA target genes, which in turn influences the normal life events of cells.

中图分类号:

S852.659.2

李畅, 李静, 何启盖. 猪圆环病毒2型感染PK-15细胞差异表达miRNA和mRNA互作网络[J]. 畜牧兽医学报, 2019, 50(1): 115-125.

LI Chang, LI Jing, HE Qigai. Integrated Analysis of miRNA and mRNA Network of Porcine Circovirus 2 Infecting Porcine Kidney Cell Line[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 115-125.

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