携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答研究

doi:10.11843/j.issn.0366-6964.2013.12.016

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 1970-1975.doi: 10.11843/j.issn.0366-6964.2013.12.016

携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答研究

熊朝丽¹，张华²，程振涛^1,3，周碧君^1,3，王开功^1,3，文明^1,3*

(1. 贵州大学动物疫病研究所，贵阳 550025；2. 贵州省动物疫病预防控制中心，贵阳 550008；3. 贵州省动物疫病与兽医公共卫生重点实验室，贵阳 550025)

收稿日期:2013-06-04 出版日期:2013-12-23 发布日期:2013-12-23
通讯作者: 文明(1969-)，男，教授，博士，E-mail：as.mwen@gzu.edu.cn，Tel:0851-8298078
作者简介:熊朝丽(1988-)，女，贵州遵义人，硕士生，主要从事兽医传染病与预防兽医学研究，Tel: 0851-8298078
基金资助:
贵州省优秀科技教育人才省长专项基金项目(黔省专合字［2006］12号)；贵州省重大科技专项计划项目(黔科合重大专项字［2011］6009号)；贵州省重点实验室培育项目(黔科合Z字［2011］4008号)

Study on Immunity Response in Goat Induced by the Attenuated Salmonella typhimurium Carrying pVAX1-GPV-P32

XIONG Chao-li¹, ZHANG Hua², CHENG Zhen-tao^{1, 3}, ZHOU Bi-jun^{1, 3},WANG Kai-gong^{1, 3}, WEN Ming^{1, 3*}

(1. Animal Disease Research Institute of Guizhou University, Guiyang 550025, China; 2. Guizhou Animal Disease Prevention and Control Center, Guiyang 550008, China; 3. Key Laboratory of Animal Disease and Veterinary Public Health of Guizhou Province, Guiyang 550025, China)

Received:2013-06-04 Online:2013-12-23 Published:2013-12-23

摘要/Abstract

摘要：

为探讨携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答效果，将60只健康黑山羊随机分为3组，即重组细菌组、弱毒疫苗组和空白对照组，重组细菌组灌服重组减毒沙门菌，弱毒疫苗组股内侧皮内注射山羊痘弱毒疫苗，空白对照组不作任何处理；于不同时间段采集山羊血液、呼吸道/消化道分泌液及组织样本进行检测。结果：(1)在免疫后 7、15、30和45 d的山羊消化道特异性 SIgA 含量中，重组细菌组明显高于弱毒疫苗组(P<0.05)，45 d时到达最高值(18.172±0.122) μg·L^-1；(2)在诱导产生特异性 P32 抗体上，重组细菌组略低于弱毒疫苗组，但差异不明显(P>0.05)；(3)在特异性淋巴细胞转化效率上，免疫15 d前重组细菌组略低于弱毒疫苗组，但免疫 30 d 后重组细菌组高于弱毒疫苗组，但差异均不显著(P>0.05)；(4)在诱导产生特异性细胞因子方面，重组细菌组山羊血清 IL-2 含量低于弱毒疫苗组，差异极显著(P<0.01)，血清 IL-5 含量高于弱毒疫苗组，差异极显著(P<0.01)，血清 TNF-β高于弱毒疫苗组，差异显著(P<0.05)，而血清 IFN-γ 和 TNF-α 含量与弱毒疫苗组差异不显著(P>0.05)。上述结果表明，构建的携带 pVAX1- GPV-P32 重组减毒沙门菌能诱导山羊产生较好的免疫应答反应。

Abstract:

The aim of this study was to study the immune response effect of the recombinant attenuated Salmonella carrying pVAX1-GPV-P32 to goats. The 60 black goats were randomly divided into three groups as recombinant bacteria group, attenuated vaccine group and the control group, which the goats were fed by the recombinant attenuated Salmonella in recombinant bacteria group, and injected by the goat pox attenuated vaccine in the attenuated vaccine group, but without any treatment in the control group. Then the samples of the blood and respiratory/digestive tract secretion and tissue were collected respectively in the different periods after immunization and used for detection and analysis. The results were showed as follows: (1)The contents of the specific SIgA in recombinant bacteria group was significant higher than that in the attenuated vaccine group (P<0.05) in 7, 15, 30 and 45 d post-immunization (PI), and which reached the highest value (18.172±0.122 μg·L^-1) in 45 d; (2) The contents of anti-GPV P32 antibody in the recombinant bacteria group were slightly lower than that in the attenuated vaccine group, but the difference between groups was not significant (P>0.05); (3)The transformation efficiency of the specific lymphocyte in the recombinant bacteria group was slightly lower than that in the attenuated vaccine group from 0 to15 d PI, but higher than that in the attenuated vaccine group in 30 d and 45 d PI, but the difference was not significant (P>0.05). (4)The IL-2 content of the goat serum in the recombinant bacteria group were less than that in the attenuated vaccine group and the difference was very significant (P<0.01), and the IL-5 levels were higher than that in the attenuated vaccine group and the difference was very significant (P<0.01) too, and the TNF-β levels were higher than that in the attenuated vaccine group and the difference was significant (P<0.05), and the content of IFN-γ and TNF-α were not significant with that in the attenuated vaccine group (P>0.05). These results indicated that the recombinant attenuated Salmonella carrying the pVAX1-GPV-P32 made the goat have a better immune responses.

中图分类号:

熊朝丽，张华，程振涛，周碧君，王开功，文明. 携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答研究[J]. 畜牧兽医学报, 2013, 44(12): 1970-1975.

XIONG Chao-li, ZHANG Hua, CHENG Zhen-tao, ZHOU Bi-jun,WANG Kai-gong, WEN Ming. Study on Immunity Response in Goat Induced by the Attenuated Salmonella typhimurium Carrying pVAX1-GPV-P32[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1970-1975.

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携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答研究

Study on Immunity Response in Goat Induced by the Attenuated Salmonella typhimurium Carrying pVAX1-GPV-P32

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