畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (7): 1167-1172.doi: 10.11843/j.issn.0366-6964.2013.07.024

• 研究简报 • 上一篇    

青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查

张斌,朱晓霞,岳华,汤承*   

  1. (西南民族大学生命科学与技术学院,成都 610041)
  • 收稿日期:2013-01-08 出版日期:2013-07-23 发布日期:2013-07-23
  • 通讯作者: 汤承,E-mail: tangcheng101@163.com
  • 作者简介:张斌(1983-),男,河南睢县人,博士,副研究员,主要从事病原分子生物的研究
  • 基金资助:

    川西北牧区“生产生态生活”优化保障关技术集成与示范(2012BAD13B06)

The Survey Study on the Serovar and Virulent Gene of Yak Salmonella from Some Areas of Qinghai-Tibetan Plateau

ZHANG Bin, ZHU Xiao-xia, YUE Hua, TANG Cheng*   

  1. (College of Life Science and Technology, Southwest University for Nationalities,Chengdu 610041, China)
  • Received:2013-01-08 Online:2013-07-23 Published:2013-07-23

摘要:

本研究旨在对中国青藏高原部分地区牦牛源沙门菌(Salmonella)的血清型和毒力基因分布频率进行调查。血清型鉴定结果显示:31株牦牛源沙门菌分为8种不同血清型,以都柏林沙门菌(10/31, 32.26%)、肠炎沙门菌(9/31, 29.03%)和鼠伤寒沙门菌(4/31, 12.90%)为主要血清型,其次为布利丹沙门菌、御成门沙门菌、斯坦利沙门菌、奥雷翁沙门菌、纽波特沙门菌。采用PCR方法对14个毒力基因分布频率进行调查,结果表明所有菌株均携带有SPI-1SPI-5毒力岛的相关基因,5种血清型牦牛源沙门菌携带毒性质粒,首次发现布利丹沙门菌和御成门沙门菌携带毒性质粒。本研究青藏高原部分地区牦牛源沙门菌毒力基因携带频率高,暗示了具有较强的致病性,为进一步研究青藏高原牦牛源沙门菌的致病性提供了参考。

Abstract:

The aim of this study was to investigate the distribution of serovars and virulent genes of yak Salmonella from the some areas of the Qinghai-Tibetan pleteau. The serotyping result exhibited that the 31 yak Salmonella isolates were divided into 8 different serovars, S. Dublin (32.26%), S. Enterica (29.03%) and S. Typhimurium (12.90%) were dominant serovars, other are S. Blegdam, S. Onarimon, S. Stanely, S. Orion and S. Newport. In addition, the 14 different virulent genes were detected in 31 yak Salmonella isolates by PCR method. The results showed all isolates carried virulent genes of the pathogenicity island-1 (SPI-1) to SPI-5, and the five serovars yak Salmonella isolates carried the virulence plasmid. It is noteworthy that the S. Blegdam and S. Onarimon were first found to possess the virulence plasmid. Therefore, the high carrying rate of virulence plasmid was occurred in yak Salmonella suggested that the yak Salmonella might have strong pathogenicity, which will provide important information to further study the pathogenicity of yak Salmonella from the Qinghai-Tibetan pleteau.

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