Abstract:

To develop vaccine for raccoon dog enteritis caused by raccoon dog parvovirus, parvovirus strains with high immunogenicity and high efficacy were selected as vaccine candidates. Live parvovirus was isolated from feces of clinical raccoon dog by CrandallReese feline kidney (CRFK) cells. The virus strains were isolated then identified with morphological methods, serological methods, molecular biological methods, animal regression experiment and immunogenic evaluation. One RDPV strain named LN10-1 was isolated successfully. Its complete VP2 sequence of LN10-1 strain had the highest identity with FPLV monkey/BJ-22/2008/CHN (99.7 percent). The two AA residues characterized as determination of host range were changed. The phylogenetic analysis of VP2 gene sequences revealed that the LN10-1 isolation was presented between the two genetic clusters, one was composed with CPV, and the other cluster was consist of carnivores parvovirus (FPLV, MEV and BFPV). The Immunological assay result shows the neutralizing antibody titre of parvovirus is higher than 64 folds on 28 day postimmunization. It is supposed that LN10-1 is evolving between FPLV and CPV, or there may be a new virus that becomes adapted to its new host (raccoon dog). And this strain could be used as a candidate inactivated vaccine for preventing haemorrhagic enteritis in Raccoon dogs.