畜禽m6A甲基化修饰及营养调控研究进展

doi:10.11843/j.issn.0366-6964.2025.09.008

摘要/Abstract

摘要： N6-甲基腺苷(m⁶A)甲基化修饰是由m⁶A甲基转移酶、m⁶A去甲基化酶和m⁶A识别蛋白共同介导的一种动态、可逆的转录后修饰，是真核生物体内最丰富的RNA修饰。近年来，m⁶A甲基化在畜禽生产中的生物学功能逐渐被揭示，其参与调控畜禽生长发育、生殖生理、糖脂代谢、氧化应激和细胞损伤等生命过程。畜禽生产中营养、环境、疾病等因素的变化会干扰机体m⁶A甲基化修饰进而影响畜禽生理代谢过程，而通过营养调控手段可恢复甲基化稳态实现对畜禽生长、发育和疾病等方面的调节。本文对上述内容进行综述，为提高畜禽生长性能、保障畜禽健康提供新的见解和思路。

关键词: m⁶A甲基化修饰, 甲基化稳态, 营养调控, 畜禽

Abstract: N6-methyladenosine (m⁶A) methylation represents a dynamic and reversible post-transcriptional modification regulated by m⁶A methyltransferases, m⁶A demethylases, and m⁶A reader proteins. It is recognized as the most prevalent RNA modification in eukaryotic organisms. Recent studies have progressively elucidated the biological roles of m⁶A methylation in livestock and poultry production, highlighting its involvement in regulating critical life processes, including growth and development, reproductive physiology, glucose and lipid metabolism, oxidative stress, and cellular damage. Alterations in factors such as nutrition, environment, and disease within livestock and poultry production can disrupt m⁶A methylation modifications, thereby impacting the physiological metabolic processes of these animals. Nonetheless, nutritional regulation strategies have the potential to restore methylation homeostasis, thereby facilitating the regulation of growth, development, and diseases in livestock and poultry. This article provides a comprehensive review of these topics, offering novel insights and strategies for enhancing growth performance and ensuring the health of livestock and poultry.

Key words: m⁶A methylation modification, methylation homeostasis, nutrients regulation, livestock and poultry

中图分类号:

S852.2

白广栋, 楼泽楷, 王瑞琪, 赵轩, 李家维, 夏耀耀, 庞家满. 畜禽m⁶A甲基化修饰及营养调控研究进展[J]. 畜牧兽医学报, 2025, 56(9): 4215-4231.

BAI Guangdong, LOU Zekai, WANG Ruiqi, ZHAO Xuan, LI Jiawei, XIA Yaoyao, PANG Jiaman. Advances in m⁶A Methylation Modification and Nutritional Regulation in Livestock and Poultry[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4215-4231.

参考文献

[1] HENGWEI Y, RAZA S H A, WENZHEN Z, et al. Research progress of m⁶A regulation during animal growth and development[J]. Mol Cell Probes, 2022, 65: 101851.
[2] MI S Y, SHI Y J, DARI G, et al. Function of m⁶A and its regulation of domesticated animals’ complex traits[J]. J Anim Sci, 2022, 100(3):skac034.
[3] 张娟丽, 杨姣姣, 杨巧丽, 等. m⁶A甲基化修饰对畜禽生产影响的研究进展[J]. 中国兽医学报, 2023, 43(6): 1333-1341.
ZHANG J L, YANG J J, YANG Q L, et al. Research progress of m⁶a methylation modification of livestock and poultry[J]. Chinese Journal of Veterinary Science, 2023, 43(6): 1333-1341.(in Chinese)
[4] MEYER K D, SALETORE Y, ZUMBO P, et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons[J]. Cell, 2012, 149(7): 1635-1646.
[5] HUANG H, WENG H, CHEN J. m⁶A modification in coding and non-coding RNAs: roles and therapeutic implications in cancer[J]. Cancer Cell, 2020, 37(3): 270-288.
[6] CAI T, ATTEH L L, ZHANG X, et al. The N6-Methyladenosine modification and its role in mRNA metabolism and gastrointestinal tract disease[J]. Front Surg, 2022, 9: 819335.
[7] SCHÖLLER E, WEICHMANN F, TREIBER T, et al. Interactions, localization, and phosphorylation of the m⁶A generating METTL3-METTL14-WTAP complex[J]. RNA, 2018, 24(4): 499-512.
[8] BAWANKAR P, LENCE T, PAOLANTONI C, et al. Hakai is required for stabilization of core components of the m⁶A mRNA methylation machinery[J]. Nat Commun, 2021, 12(1):3778.
[9] KARANDASHOV I K A, DUKICH M, PONOMAREVA N, et al. m⁶A methylation in regulation of antiviral innate immunity[J]. Viruses 2024, 16, 601.
[10] GUIMARAES-TEIXEIRA C, BARROS-SILVA D, LOBO J, et al. Deregulation of N6-methyladenosine RNA modification and its erasers FTO/ALKBH5 among the main renal cell tumor subtypes[J]. J Pers Med, 2021, 11(10):996.
[11] JIA G, FU Y, ZHAO X, et al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO[J]. Nat Chem Biol, 2011, 7(12): 885-887.
[12] KAUR S, TAM N Y, MCDONOUGH M A, et al. Mechanisms of substrate recognition and N6-methyladenosine demethylation revealed by crystal structures of ALKBH5-RNA complexes[J]. Nucleic Acids Res, 2022, 50(7): 4148-4160.
[13] ALARCON C R, GOODARZI H, LEE H, et al. HNRNPA2B1 is a mediator of m⁶A-dependent nuclear RNA processing events[J]. Cell, 2015, 162(6): 1299-1308.
[14] HUANG H, WENG H, SUN W, et al. Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation[J]. Nat Cell Biol, 2018, 20(3): 285-295.
[15] YU T, QI X, ZHANG L, et al. Dynamic reprogramming and function of RNA N6-methyladenosine modification during porcine early embryonic development[J]. Zygote, 2021, 29(6): 417-426.
[16] COLLIGNON E, CHO B, FURLAN G, et al. m⁶A RNA methylation orchestrates transcriptional dormancy during paused pluripotency[J]. Nat Cell Biol, 2023, 25(9): 1279-1289.
[17] 丁浩, 林月月, 张涛, 等. m⁶A甲基化在鸡肌肉生长发育中的表达研究[J]. 中国畜牧兽医, 2021, 48(5): 1525-1534.
DING H, LIN Y Y, ZHANG T, et al.Study on the expression of m⁶A methylation in chicken muscle growth and development[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(5): 1525-1534. (in Chinese)
[18] ZHANG D, WU S, ZHANG X, et al. Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs[J]. J Anim Sci Biotechnol, 2022, 13(1): 146.
[19] GU L, ZHANG S, LI B, et al. m⁶A and miRNA jointly regulate the development of breast muscles in duck embryonic stages[J]. Front Vet Sci, 2022, 9: 933850.
[20] DANG Y, DONG Q, WU B, et al. global landscape of m⁶A methylation of differently expressed genes in muscle tissue of Liaoyu white cattle and Simmental cattle[J]. Front Cell Dev Biol, 2022, 10: 840513.
[21] HE S, WANG H, LIU R, et al. mRNA N6-methyladenosine methylation of postnatal liver development in pig[J]. PLoS One, 2017, 12(3): e0173421.
[22] MA C, CHANG M, LV H, et al. RNA m⁶A methylation participates in regulation of postnatal development of the mouse cerebellum[J]. Genome Biol, 2018, 19(1):68.
[23] ZHOU W, XUE P, YANG Y, et al. Research progress on N6-methyladenosine in the human placenta[J]. J Perinat Med, 2022, 50(8): 1115-1123.
[24] WANG S, ZHANG L, XUAN R, et al. Identification and functional analysis of m⁶A in the mammary gland tissues of dairy goats at the early and peak lactation stages[J]. Front Cell Dev Biol, 2022, 10: 945202.
[25] WANG Y, ZHENG Y, GUO D, et al. m⁶A methylation analysis of differentially expressed genes in skin tissues of coarse and fine type Liaoning Cashmere goats[J]. Frontiers in Genet, 2020, 10:1318.
[26] CHEN Y, WANG J, XU D, et al. m⁶A mRNA methylation regulates testosterone synthesis through modulating autophagy in leydig cells[J]. Autophagy, 2021, 17(2): 457-475.
[27] LIN Z, HSU P J, XING X, et al. METTL3-/METTL14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis[J]. Cell Res, 2017, 27(10): 1216-1230.
[28] XUESONG SUI A K, AND LU GAO. RNA m⁶A modifications in mammalian gametogenesis and pregnancy[J]. Reproduction, 2022,165(1):R1-R8.
[29] DU L, LI Y, KANG M, et al. USP48 is upregulated by METTL14 to attenuate hepatocellular carcinoma via regulating SIRT6 stabilization[J]. Cancer Res, 2021, 81(14): 3822-3834.
[30] LI Y, HE L, WANG Y, et al. N6-methyladenosine methyltransferase KIAA1429 elevates colorectal cancer aerobic glycolysis via HK2-dependent manner[J]. Bioengineered, 2022, 13(5): 11923-11932.
[31] LI H, ZHANG N, JIAO X, et al. Downregulation of microRNA-6125 promotes colorectal cancer growth through YTHDF2-dependent recognition of N6-methyladenosine-modified GSK3β[J]. Clin transl med, 2021, 11(10): e602.
[32] CHEN B, HONG Y, GUI R, et al. N6-methyladenosine modification of circ_0003215 suppresses the pentose phosphate pathway and malignancy of colorectal cancer through the miR-663b/DLG4/G6PD axis[J]. Cell Death Dis, 2022, 13(9):804.
[33] LIU J, LUO G, SUN J, et al. METTL14 is essential for β-cell survival and insulin secretion[J]. Biochim Biophys Acta Mol Basis Dis, 2019, 1865(9): 2138-2148.
[34] 江芹. mRNA m⁶A修饰关键基因对猪肌内脂肪沉积的影响及机制[D]. 杭州：浙江大学, 2018.
JIANG Q. Effects and mechanisms of key mRNA m⁶A genes in porcine intramuscular fat deposition[D]. Hangzhou: Zhejiang University, 2018.(in Chinese)
[35] CHAO M, WANG M, HAN H, et al. Profiling of m⁶A methylation in porcine intramuscular adipocytes and unravelling PHKG1 represses porcine intramuscular lipid deposition in an m⁶A-dependent manner[J]. Int J Biol Macromol, 2024, 272(Pt 1):132728.
[36] LUO G, WANG S, AI Y, et al. N6-methyladenosine methylome profiling of muscle and adipose tissues reveals methylase-mRNA metabolic regulatory networks in fat deposition of Rex rabbits[J]. Biology, 2022, 11(7):944.
[37] LI K, HUANG W, WANG Z, et al. m⁶A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte[J]. J Anim Sci Biotechnol, 2022, 13(1):147.
[38] 韩皓哲, 帖子航, 庞卫军, 等. IGF2BP2介导的m⁶A修饰调控动物脂肪沉积的研究进展[J]. 畜牧兽医学报, 2023, 54(9): 3605-3612.
HAN H Z,TIE Z H, PANG W J, et al. Advances of IGF2BP2-mediated m⁶A modification on animal fat deposition[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3605-3612.(in Chinese)
[39] DUAN Y, LV X, CAO X, et al. Effect of METTL3 gene on lipopolysaccharide induced damage to primary small intestinal epithelial cells in sheep[J]. Int J Mol Sci, 2024, 25(17): 9316.
[40] LI S, WANG Y, XU A, et al. Dietary selenomethionine reduced oxidative stress by resisting METTL3-mediated m⁶A methylation level of Nrf2 to ameliorate LPS-induced liver necroptosis in laying hens[J]. J Nutr Biochem, 2024, 125: 109563.
[41] XU H, LIN C, WANG C, et al. ALKBH5 stabilized N6-methyladenosine-modified LOC4191 to suppress E.coli-induced apoptosis[J]. Cells, 2023, 12(22):2604.
[42] FENG Y, SHEN J, LIN Z, et al. PXR activation relieves deoxynivalenol-induced liver oxidative stress via MALAT1 lncRNA m⁶A demethylation[J]. Adv Sci, 2024, 11(25):e2308742.
[43] YANG J, ZHANG J, GAO X, et al. FTO regulates apoptosis in CPB2-treated IPEC-J2 cells by targeting caspase 3 apoptotic protein[J]. Animals (Basel), 2022, 12(13):1644.
[44] XU H, LIN C, LI T, et al. N6-methyladenosine-modified circRNA in the bovine mammary epithelial cells injured by Staphylococcus aureus and Escherichia coli[J]. Front Immunol, 2022, 13: 873330.
[45] 束婧婷, 单艳菊, 姬改革, 等. 广西麻鸡m⁶A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J]. 中国农业科学, 2022, 55(3): 13.
SHU J T, SHAN Y J, JI G G, et al. Relationship between expression levels of Guangxi Partridge chicken m⁶A Methyltransferase genes, myofiber types and myogenic differentiation[J]. Scientia Agricultura Sinica, 2022, 55(3): 13. (in Chinese)
[46] CAO Y, ZHANG S, WANG G, et al. Expression analysis of m⁶A-related genes in various tissues of Meishan pigs at different developmental stages[J]. R Bra Zootec, 2023, 52：e20210149.
[47] 王明会. m⁶A修饰在高脂日粮诱导的肉鸡和小鼠糖脂代谢紊乱中的作用[D].泰安：山东农业大学, 2022.
WANG M H. Effects of m⁶A modification in high-fat diet-Induced the disorders of glucose and lipid metabolism in broilers and mice[D]. Taian: Shandong Agricultural University, 2022. (in Chinese)
[48] GEBEYEW K, YANG C, MI H, et al. Lipid metabolism and m⁶A RNA methylation are altered in lambs supplemented rumen-protected methionine and lysine in a low-protein diet[J]. J. Anim Sci Biotechnol, 2022, 13(1):85.
[49] CAI S, QUAN S, YANG G, et al. Nutritional status impacts epigenetic regulation in early embryo development: A scoping Review[J]. Adv Nutr, 2021, 12(5): 1877-1892.
[50] SONG T, LU J, DENG Z, et al. Maternal obesity aggravates the abnormality of porcine placenta by increasing N⁶-methyladenosine[J]. Int J Obes (Lond), 2018, 42(10): 1812-1820.
[51] 丰伟丽, 薛智慧, 赵庆博, 等. 呕吐毒素体外刺激3D4/21猪肺泡巨噬细胞对炎性因子和m⁶A甲基化相关基因表达的影响[J]. 中国畜牧杂志, 2024, 60(7): 295-303.
FENG W L, XUE Z H, ZHAO Q B, et al. Effect of in vitro stimulation of 3D4/21 porcine alveolar macrophages with DON on the expression of inflammatory factors and m⁶A methylation-related genes[J]. Chinese Journal of Animal Science, 2024, 60(7): 295-303. (in Chinese)
[52] 刘亮, 苏晓彤, 李慧贤, 等. 玉米赤霉烯酮对湖羊睾丸间质细胞毒性及m⁶A甲基化修饰相关基因表达的影响[J]. 南京农业大学学报, 2023, 46(4): 772-779.
LIU L, SU X T, LI H X, et al. Effects of zearalenone on cytotoxicity and m6A methylation related gene expression in leydig cells of Hu sheep[J]. Journal of Nanjing Agricultural University, 2023, 46(4): 772-779. (in Chinese)
[53] WU K, JIA S, ZHANG J, et al. Transcriptomics and flow cytometry reveals the cytotoxicity of aflatoxin B₁ and aflatoxin M₁ in bovine mammary epithelial cells[J]. Ecotoxicol Environ Saf, 2021, 209:111823.
[54] DING H, LI Z, LI X, et al. FTO alleviates CdCl₂-induced apoptosis and oxidative stress via the AKT/Nrf2 pathway in bovine granulosa cells[J]. Int J Mol Sci, 2022, 23(9):4948.
[55] LI W, TAN M, WANG H, et al. METTL3-mediated m⁶A mRNA modification was involved in cadmium-induced liver injury[J]. Environ Pollut, 2023, 331: 121887.
[56] SUN Y, LIU G, LI M, et al. Study on the correlation between regulatory proteins of N6-methyladenosine and oxidative damage in cadmium-induced renal injury[J]. Biol Trace Elem Res, 2023, 201(5): 2294-302.
[57] BAI L, TANG Q, ZOU Z, et al. m⁶A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite[J]. Toxico Sci, 2018, 165(2): 431-446.
[58] GU S, SUN D, DAI H, et al. N6-methyladenosine mediates the cellular proliferation and apoptosis via microRNAs in arsenite-transformed cells[J]. Toxico Lett, 2018, 292: 1-11.
[59] WANG C, SUN H, JIANG X, et al. Maternal oxidized soybean oil administration in rats during pregnancy and lactation alters the intestinal DNA methylation in offspring[J]. J Agric Food Chem, 2022, 70(20): 6224-6238.
[60] CAYIR A, BYUN H M, BARROW T M. Environmental epitranscriptomics[J]. Environ Res, 2020, 189: 109885.
[61] HE F, MU X, ZHANG Y, et al. Late gestational exposure to fenvalerate impacts ovarian reserve in neonatal mice via YTHDF2-mediated P-body assembly[J]. Sci Total Environ, 2024, 925: 171790.
[62] CHEN N, TANG J, SU Q, et al. Paraquat-induced oxidative stress regulates N6-methyladenosine (m⁶A) modification of circular RNAs[J]. Environ Pollut, 2021, 290: 117816.
[63] WANG C, XU J, LUO S, et al. Parental exposure to environmentally relevant concentrations of bisphenol-A bis(diphenyl phosphate) impairs vascular development in offspring through DNA/RNA methylation-dependent transmission[J]. Environ Sci Technol, 2023, 57(43): 16176-16189.
[64] WANG W, LI X, QIAN Q, et al. Mechanistic exploration on neurodevelopmental toxicity induced by upregulation of ALKBH5 targeted by triclosan exposure to larval zebrafish[J]. J Hazard Mater, 2023, 457: 131831.
[65] QI Y, ZHANG Y, ZHANG J, et al. The alteration of N6-methyladenosine (m⁶A) modification at the transcriptome-wide level in response of heat stress in bovine mammary epithelial cells[J]. BMC Genomics, 2022, 23(1):829.
[66] LU Z, MA Y, LI Q, et al. The role of N6-methyladenosine RNA methylation in the heat stress response of sheep (Ovis aries)[J]. Cell Stress Chaperones, 2019, 24(2): 333-342.
[67] CHEN B, YUAN C, GUO T, et al. Molecular mechanism of m⁶A methylation modification genes METTL3 and FTO in regulating heat stress in sheep[J]. Int J Mol Sci, 2023, 24(15):11926.
[68] 李笑微, 田微, 刘媛, 等. 高温应激下湖羊卵巢颗粒细胞m⁶A甲基化修饰差异研究[J]. 畜牧兽医学报, 2025, 56(4): 1712-1721.
LI X W, TIAN W, LIU Y, et al. Study on the difference of m⁶A methylation modification in ovarian granulosa cells of Hu Sheep under heat stress[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1712-1721. (in Chinese)
[69] KISLIOUK T, ROSENBERG T, BEN-NUN O, et al. Early-life m⁶A RNA demethylation by fat mass and obesity-associated protein (FTO) influences resilience or vulnerability to heat stress later in life[J]. eNeuro, 2020, 7(3): ENEURO.0549-19.2020.
[70] SUN M H, JIANG W J, LI X H, et al. High temperature-induced m⁶A epigenetic changes affect early porcine embryonic developmental competence in pigs[J]. Microsc Microanal, 2023, 29(6): 2174-2183.
[71] HENG J, TIAN M, ZHANG W, et al. Maternal heat stress regulates the early fat deposition partly through modification of m⁶A RNA methylation in neonatal piglets[J]. Cell Stress Chaperones, 2019, 24(3): 635-645.
[72] CAYIR A, BARROW T M, GUO L, et al. Exposure to environmental toxicants reduces global N6-methyladenosine RNA methylation and alters expression of RNA methylation modulator genes[J]. Environ Res, 2019, 175: 228-234.
[73] JI C, TAO Y, LI X, et al. AHR-mediated m⁶A RNA methylation contributes to PM_2.5-induced cardiac malformations in zebrafish larvae[J]. J Hazard Mater, 2023, 457: 131749.
[74] HE X, ZHANG L, LIU S, et al. Methyltransferase-like 3 leads to lung injury by up-regulation of interleukin 24 through N6-methyladenosine-dependent mRNA stability and translation efficiency in mice exposed to fine particulate matter 2.5[J]. Environ Pollut, 2022, 308: 119607.
[75] XING Y, TANG Y, CHEN Q, et al. The role of RNA epigenetic modification-related genes in the immune response of cattle to mastitis induced by Staphylococcus aureus[J]. Anim Biosci, 2024, 37(7): 1141-1155.
[76] 李婷. RNA m⁶A修饰调控金黄色葡萄球菌及大肠杆菌诱导的奶牛乳腺上皮细胞炎症反应机制[D].武汉：华中农业大学，2021.
LI T. RNA Regulatory mechanism of RNA m⁶A modification on inflammatory response of bovine mammary epithelial cells induced by Staphylococcus aureus/Escherichia coli[D]. Wuhan: Huazhong Agricultural University, 2021. (in Chinese)
[77] JIN J, LIU M, YU F, et al. METTL3 enhances E. coli F18 resistance by targeting IKBKG/NF-κB signaling via an m⁶A-YTHDF1-dependent manner in IPEC-J2 cells[J]. Int J Biol Macromol, 2024, 262(Pt 2): 130101.
[78] 王诗琴. METTL3介导m⁶A修饰对断奶仔猪F18大肠杆菌抗性的调控机制分析[D].扬州：扬州大学，2021.
WANG S Q. Analysis of the regulatory mechanism of METTL3-mediated m⁶A modification on E.coli F18 resistance in weaned piglets[D]Yangzhou: Yangzhou University, 2021. (in Chinese)
[79] WU Z, WANG Y, LI T, et al. Comprehensive analysis revealed the potential roles of N6-methyladenosine (m⁶A) mediating E. coli F18 susceptibility in IPEC-J2 Cells[J]. Int J Mol Sci, 2022, 23(21):13602.
[80] ZONG X, WANG H, XIAO X, et al. Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m⁶A-GPR161 signalling axis[J]. RNA Biol, 2021, 18(4): 576-586.
[81] 张娟丽. m⁶A修饰在CPB2毒素诱导猪IPEC-J2细胞损伤中的功能机制研究[D].兰州：甘肃农业大学，2022.
ZHANG J L. Functional mechanism of m⁶A modification in CPB2 toxin-indued injury of porcine IPEC-J2 cells[D].Lanzhou: Gansu Agricultural University, 2022. (in Chinese)
[82] 薛智慧, 郭鑫, 李水莲, 等. 副猪嗜血杆菌体外刺激3D4/21猪肺泡巨噬细胞对炎性因子和m⁶A相关基因表达的影响[J]. 黑龙江畜牧兽医, 2022(7): 71-75.
XUE Z H, GUO X, LI S L, et al. Effect of in vitro stimulation of Haemophilus parasuis on the expression of inflammatory factors and m⁶A-related genes in porcine alveolar macrophage 3D4 /21 cells[J]. Heilongjiang Animal Science and Veterinary Medicine, 2022(7): 71-75. (in Chinese)
[83] ZHANG J, YANG J, GAO X, et al. METTL3 regulates the inflammatory response in CPB2 toxin-exposed IPEC-J2 cells through the TLR2/NF-κB signaling pathway[J]. Int J Mol Sci, 2022, 23(24):15833.
[84] GONG X, LIANG Y, WANG J, et al. Highly pathogenic PRRSV upregulates IL-13 production through nonstructural protein 9-mediated inhibition of N6-methyladenosine demethylase FTO[J]. J Biol Chem, 2024, 300(4): 107199.
[85] CHEN J, SONG H X, HU J H, et al. Classical swine fever virus non-structural protein 5B hijacks host METTL14-mediated m⁶A modification to counteract host antiviral immune response[J]. PLoS Pathog, 2024, 20(3): e1012130.
[86] JIN J, XU C, WU S, et al. m⁶A demethylase ALKBH5 restrains PEDV infection by regulating GAS6 expression in porcine alveolar macrophages[J]. Int J Mol Sci, 2022, 23(11):6191.
[87] LI C, WU X, YANG Y, et al. METTL14 and FTO mediated m⁶A modification regulate PCV2 replication by affecting miR-30a-5p maturity[J]. Virulence, 2023, 14(1): 2232910.
[88] NABI KHAN R I, PRAHARAJ M R, MALLA W A, et al. Changes in m⁶A RNA methylation of goat lung following PPRV infection[J]. Heliyon, 2023, 9(9): e19358.
[89] WU L, QUAN W, ZHANG Y, et al. Attenuated duck hepatitis A virus infection is associated with high mRNA maintenance in duckling liver via m⁶A modification[J]. Front Immunol, 2022, 13: 839677.
[90] WU Y C, LIU X, WANG J L, et al. Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells[J]. Oncol Lett, 2018, 15(3): 3109-3120.
[91] FU R, CHEN D, TIAN G, et al. Betaine affects abdominal flare fat metabolism via regulating m⁶A RNA methylation in finishing pigs fed a low-energy diet[J]. J Funct Foods, 2023, 107:105620.
[92] KANG H, ZHANG Z, YU L, et al. FTO reduces mitochondria and promotes hepatic fat accumulation through RNA demethylation[J]. J Cell Biochem, 2018, 119(7): 5676-5685.
[93] WANG Y K, YU X X, LIU Y H, et al. Reduced nucleic acid methylation impairs meiotic maturation and developmental potency of pig oocytes[J]. Theriogenology, 2018, 121: 160-167.
[94] LI J, FANG L, XI M, et al. Toxic effects of triclosan on hepatic and intestinal lipid accumulation in zebrafish via regulation of m⁶A-RNA methylation[J]. Aquat Toxicol, 2024, 269: 106884.
[95] LI N, ZHANG D, CAO S, et al. The effects of folic acid on RNA m⁶A methylation in hippocampus as well as learning and memory ability of rats with acute lead exposure[J]. J Funct Foods, 2021, 76:104276.
[96] LU N, LI X, YU J, et al. Curcumin attenuates lipopolysaccharide-induced hepatic lipid metabolism disorder by modification of m⁶A RNA methylation in piglets[J]. Lipids, 2018, 53(1): 53-63.
[97] 卓儒浩. m⁶A RNA甲基化介导猪轮状病毒在肠道中感染的机制及姜黄素的营养调控研究[D].南京：南京农业大学，2022.
ZHUO R H. The mechanism of m⁶A RNA methylation mediating porcine rotavirus infection in intestine and the nutritional regulation of curcumin[D].Nanjing: Nanjing Agricultural University, 2022. (in Chinese)
[98] CHEN Y, WU R, CHEN W, et al. Curcumin prevents obesity by targeting TRAF4-induced ubiquitylation in m⁶A-dependent manner[J]. EMBO Rep, 2021, 22(5):e52146.
[99] GAN Z, WEI W, WU J, et al. Resveratrol and curcumin improve intestinal mucosal integrity and decrease m⁶A RNA methylation in the intestine of weaning piglets[J]. ACS Omega, 2019, 4(17): 17438-17446.
[100] QIAO Y, XIAO G, ZHU X, et al. Resveratrol enhances antioxidant and anti-apoptotic capacities in chicken primordial germ cells through m⁶A methylation: A preliminary investigation[J]. Animals (Basel), 2024, 14(15):2214.
[101] WU J, GAN Z, ZHUO R, et al. Resveratrol attenuates aflatoxin B₁-induced ROS formation and increase of m⁶A RNA methylation[J]. Animals, 2020, 10(4):677.
[102] ZHANG M, LIU J, YU C, et al. Berberine regulation of cellular oxidative stress, apoptosis and autophagy by modulation of m⁶A mRNA methylation through targeting the Camk1db/ERK pathway in zebrafish-hepatocytes[J]. Antioxidants (Basel), 2022, 11(12):2370.
[103] LIU R, WANG Q, LI Y, et al. Ginsenoside Rg1 alleviates sepsis-induced acute lung injury by reducing FBXO3 stability in an m⁶A-dependent manner to activate PGC-1α/Nrf2 signaling pathway[J]. Aaps j, 2024, 26(3): 47.
[104] 韩笑, 王梦斐, 蒲位凌, 等. 中药活性成分通过调节m⁶A甲基化修饰抗肿瘤的研究进展[J]. 中草药, 2024, 55(6): 2123-2130.
HAN X, WANG M F, PU W L, et al. Advances in anti-cancer research of active ingredients of traditional Chinese medicine through regulation of m⁶A methylation modification[J]. Chinese Traditional and Herbal Drugs, 2024, 55(6): 2123-2130. (in Chinese)
[105] LI T, TAN Y-T, CHEN Y-X, et al. Methionine deficiency facilitates antitumour immunity by altering m⁶A methylation of immune checkpoint transcripts[J]. Gut, 2023, 72(3): 501-511.
[106] SHIMA H, MATSUMOTO M, ISHIGAMI Y, et al. S-Adenosylmethionine synthesis is regulated by selective N6-Adenosine methylation and mRNA degradation involving METTL16 and YTHDC1[J]. Cell Rep, 2017, 21(12): 3354-3363.
[107] HENG J, WU Z, TIAN M, et al. Excessive BCAA regulates fat metabolism partially through the modification of m⁶A RNA methylation in weanling piglets[J]. Nutr Metab(Lond), 2020, 17:10.
[108] WANG Z, CAI D, LI K, et al. Transcriptome analysis of the inhibitory effect of cycloleucine on myogenesis[J]. Poult Sci, 2022, 101(12):102219.
[109] ZHANG M, ZHANG S, ZHAI Y, et al. Cycloleucine negatively regulates porcine oocyte maturation and embryo development by modulating N6-methyladenosine and histone modifications[J]. Theriogenology, 2022, 179: 128-140.
[110] YANG Y W, CHEN L, MOU Q, et al. Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming[J]. Theriogenology, 2020, 158: 309-320.
[111] YU X X, LIU Y H, LIU X M, et al. Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes[J]. Sci Rep, 2018, 8(1):6132.
[112] 靳艾. RNA m⁶A修饰在LPS诱导的肺部炎症中的作用机制及维生素D₃的缓解作用[D].杨凌：西北农林科技大学，2022.
JIN A. The effect and mechanism of RNA N6-methyladenosine modification in LPS-induced pulmonary inflammation and the effect of vitamin D₃ on its mitigation[D]. Yangling: Northwest A&F University, 2022. (in Chinese)
[113] MOSCA P, ROBERT A, ALBERTO J M, et al. Vitamin B₁₂ deficiency dysregulates m⁶A mRNA methylation of genes involved in neurological functions[J]. Mol Nutr Food Res, 2021, 65(17):e2100206.
[114] WANG S, LU H, SU M, et al. Bisphenol H exposure disrupts Leydig cell function in adult rats via oxidative stress-mediated m⁶A modifications: Implications for reproductive toxicity[J]. Ecotoxicol Environ Saf, 2024, 285: 117061.
[115] LIU K, HE X, HUANG J, et al. Short-chain fatty acid-butyric acid ameliorates granulosa cells inflammation through regulating METTL3-mediated N6-methyladenosine modification of FOSL2 in polycystic ovarian syndrome[J]. Clin Epigenetics, 2023, 15(1): 86.
[116] CHEN W, CHEN Y, WU R, et al. DHA alleviates diet-induced skeletal muscle fiber remodeling via FTO/m⁶A/DDIT4/PGC1α signaling[J]. BMC Biology, 2022, 20(1):39.
[117] LI Y, MA B, WANG Z, et al. The effect mechanism of N6-adenosine methylation (m⁶A) in melatonin regulated LPS-induced colon inflammation[J]. Int J Biol Sci, 2024, 20(7): 2491-2506.
[118] HU C, JI F, LV R, et al. Putrescine promotes MMP9-induced angiogenesis in skeletal muscle through hydrogen peroxide/METTL3 pathway[J]. Free Radic Biol Med, 2024, 212: 433-447.
[119] GAO J, LI Y, LIU Z, et al. Acetaminophen changes the RNA m⁶A levels and m⁶A-related proteins expression in IL-1β-treated chondrocyte cells[J]. BMC Mol Cell Biol, 2022, 23(1): 45.

畜禽m⁶A甲基化修饰及营养调控研究进展

Advances in m⁶A Methylation Modification and Nutritional Regulation in Livestock and Poultry

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨鑫, 王绍宇, 童畅, 彭志弢, 蔡圣煌, 黄俊雄, 胥娇娇, 温馨, 吴银宝. 畜禽粪污源抗生素抗性基因的水平转移研究进展[J]. 畜牧兽医学报, 2025, 56(9): 4279-4293.
[2]	张帆, 曾威, 周傲. 畜禽基因编辑抗病育种研究进展[J]. 畜牧兽医学报, 2025, 56(7): 3047-3056.
[3]	秦小霞, 甘海清, 佘高进, 刘勇, 黄兴国, 陈丽蓉, 杨玲媛. 油茶籽粕的活性成分、生物学功能及其在畜禽生产中的应用研究进展[J]. 畜牧兽医学报, 2025, 56(5): 2070-2081.
[4]	李笑微, 田微, 刘媛, 李惠侠. 高温应激下湖羊卵巢颗粒细胞m⁶A甲基化修饰差异研究[J]. 畜牧兽医学报, 2025, 56(4): 1712-1721.
[5]	王卓, 赵雨薇, 屠焰, 刁其玉, 崔凯. β-防御素的生物学特性及其在调控动物肠道屏障中的研究进展[J]. 畜牧兽医学报, 2025, 56(3): 995-1005.
[6]	常萱, 魏冰妮, 张小丽, 赵中权, 陈俊材. 畜禽胃肠道共生真菌研究进展[J]. 畜牧兽医学报, 2025, 56(1): 63-73.
[7]	王进部, 李佳, 任德明, 王立贤, 王立刚. 机器学习在畜禽基因组选择中的应用进展[J]. 畜牧兽医学报, 2024, 55(7): 2775-2785.
[8]	王亚鑫, 王璟, 田学凯, 杨公社, 于太永. 多组学技术在畜禽重要经济性状研究中的应用[J]. 畜牧兽医学报, 2024, 55(5): 1842-1853.
[9]	段益欣, 张林云, 赵永聚. SNP遗传力估计方法、影响因素及其在畜禽育种中的应用[J]. 畜牧兽医学报, 2024, 55(5): 1854-1865.
[10]	尚恺圆, 江明锋, 官久强, 安添午, 赵洪文, 柏琴, 吴伟生, 李华德, 谢荣清, 沙泉, 罗晓林, 张翔飞. 围产期母体营养调控对犊牦牛生长发育、血清生化及免疫功能的影响[J]. 畜牧兽医学报, 2024, 55(4): 1638-1648.
[11]	郭妍婷, 周建民, 王晶, 齐广海. 产蛋鸡子宫部钙离子转运及调控的研究进展[J]. 畜牧兽医学报, 2024, 55(1): 39-50.
[12]	李珂, 王宇龙, 李栋, 史新娥, 杨公社, 于太永. 畜禽泛基因组研究进展[J]. 畜牧兽医学报, 2023, 54(9): 3595-3604.
[13]	吴志立, 姚军虎, 雷新建. 过瘤胃葡萄糖对围产期奶畜营养调控的研究进展[J]. 畜牧兽医学报, 2023, 54(8): 3173-3182.
[14]	禹世雄, 魏凌云, 徐甜甜, 焦金真, 蒋林树, 贺志雄. 幼龄反刍动物肠道微生物定植规律及其营养调控研究进展[J]. 畜牧兽医学报, 2023, 54(7): 2701-2707.
[15]	冯伟民, 刘潇, 黄腾. 畜禽疱疹病毒逃避CTL识别的策略:干扰MHC-Ⅰ分子抗原递呈途径[J]. 畜牧兽医学报, 2023, 54(6): 2241-2251.