一例猫鼻神经内分泌恶性肿瘤的病理和免疫组化分析

doi:10.11843/j.issn.0366-6964.2025.06.037

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (6): 2948-2956.doi: 10.11843/j.issn.0366-6964.2025.06.037

一例猫鼻神经内分泌恶性肿瘤的病理和免疫组化分析

郑艺蕾¹^,²(), 沈冯婷¹^,²(), 辛良¹, 袁希望¹, 林琳³, 曹迪³, 吴旧生¹^,², 师福山¹^,², 王华南¹^,²^,*()

1. 浙江大学动物医学中心教学动物医院, 杭州 310030
2. 浙江大学动物科学学院动物医学系, 杭州 310030
3. 元医(杭州)科技有限公司元医动物医学诊断实验室, 杭州 310059

收稿日期:2024-07-23 出版日期:2025-06-23 发布日期:2025-06-25
通讯作者: 王华南 E-mail:yileizheng@zju.edu.cn;22317101@zju.edu.cn;hnwang@zju.edu.cn
作者简介:郑艺蕾(1995-)，女，四川成都人，副教授，博士，主要从事小动物临床诊疗研究，E-mail: yileizheng@zju.edu.cn
沈冯婷(2000-)，女，浙江杭州人，硕士生，主要从事临床兽医学研究，E-mail: 22317101@zju.edu.cn
第一联系人：
郑艺蕾与沈冯婷为同等贡献作者
基金资助:
杭州城西科创大走廊创新发展专项资助(B231220.0005-46)；深圳市瑞鹏基金会与新瑞鹏宠物医疗集团科研基金项目(2021-KYY-683000-0002)

Pathological and Immunohistochemical Analysis of A Case of Feline Nasal Malignant Neuroendocrine Neoplasm

ZHENG Yilei¹^,²(), SHEN Fengting¹^,²(), XIN Liang¹, YUAN Xiwang¹, LIN Lin³, CAO Di³, WU Jiusheng¹^,², SHI Fushan¹^,², WANG Huanan¹^,²^,*()

1. Veterinary Teaching Hospital, Center for Veterinary Sciences, Zhejiang University, Hangzhou 310030, China
2. Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310030, China
3. MetaHealth Veterinary Diagnostic Laboratory, Hangzhou 310059, China

Received:2024-07-23 Online:2025-06-23 Published:2025-06-25
Contact: WANG Huanan E-mail:yileizheng@zju.edu.cn;22317101@zju.edu.cn;hnwang@zju.edu.cn

摘要/Abstract

摘要：

本文旨在报道1例猫鼻神经内分泌恶性肿瘤的病理和免疫组化特征。4岁雄性英国短毛猫，在他院按肺炎治疗一个月未见好转遂转至本院，检查发现鼻部肿块，手术活检取鼻腔肿块进行组织病理学和免疫组化检查。组织病理学结果显示肿瘤细胞大小形态不一，异型性明显，细胞核多呈泡状，且已侵袭至周围组织；免疫组化结果显示神经元特异性烯醇化酶(neuron specific enolase，NSE)、突触素(synaptophysin，Syn)、血管活性肠肽(vasoactive intestinal peptide，VIP)、CD99(cluster of differentiation 99，CD99)呈阳性。根据实验室检查、组织病理学以及免疫组化结果，最后诊断为鼻神经内分泌恶性肿瘤，更倾向于低分化神经内分泌癌(neuroendocrine carcinoma，NEC)。本病例可为诊断该肿瘤并与其他鼻腔肿瘤相区分提供参考依据。

关键词: 猫, 神经内分泌肿瘤, 组织病理学, 免疫组化

Abstract:

The purpose of this article is to report the pathologic and immunohistochemical features of a case of feline nasal malignant neuroendocrine neoplasm. A 4-year-old male British shorthair cat was referred to our hospital after being treated for pneumonia for one month. A nasal mass was found on examination, and a surgical biopsy was performed to remove the nasal mass for histopathological and immunohistochemical examination. A biopsy of the nasal mass was performed for histopathological and immunohistochemical examination. Histopathological findings indicate that the tumor cells exhibit significant variability in size and shape, with marked atypia. The nuclei are predominantly vesicular, and the tumor has invaded surrounding tissues. Immunohistochemical results show positive staining for neuron-specific enolase (NSE), synaptophysin (Syn), vasoactive intestinal peptide (VIP), and CD99 (cluster of differentiation 99, CD99). Based on laboratory tests, histopathology and immunohistochemistry, the final diagnosis was nasal malignant neuroendocrine neoplasm and more inclined to poorly differentiated neuroendocrine carcinoma (NEC). This case hoping to provide a reference for diagnosing this tumor and differentiating it from other nasal cavity tumors.

Key words: cat, neuroendocrine neoplasm, histopathology, immunohistochemistry

中图分类号:

S857.4

郑艺蕾, 沈冯婷, 辛良, 袁希望, 林琳, 曹迪, 吴旧生, 师福山, 王华南. 一例猫鼻神经内分泌恶性肿瘤的病理和免疫组化分析[J]. 畜牧兽医学报, 2025, 56(6): 2948-2956.

ZHENG Yilei, SHEN Fengting, XIN Liang, YUAN Xiwang, LIN Lin, CAO Di, WU Jiusheng, SHI Fushan, WANG Huanan. Pathological and Immunohistochemical Analysis of A Case of Feline Nasal Malignant Neuroendocrine Neoplasm[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2948-2956.

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项目 Project	结果 Result	参考值 Reference value	单位 Unit
红细胞分布宽度 Red distribution width (RDW)	29.4↑	15.0~27.0	%
血小板Platelet (PLT)	0.23↓	1.51~6.00	10⁵·μL^-1
降钙素原Procalcitonin (PCT)	0.04↓	0.17~0.86	%
活化部分凝血酶时间 Activated partial thromboplastin time (aPTT)	25.0↓	60.0~115.0	s

抗体 Antigen	种属来源 Host species	克隆性 Clonality	稀释比 Dilution	货号 Product number
神经元特异性烯醇化酶 Neuron specific enolase (NSE)	兔 Rabbit	多克隆 Poly	1:500	GB11376-1
嗜铬粒蛋白A Chromorganin A (CgA)	兔 Rabbit	多克隆 Poly	1:1 000	GB111316
突触素 Synaptophysin (Syn)	鼠 Mouse	多克隆 Poly	1:500	GB15553
细胞角蛋白 Cytokeratin (CK)	鼠 Mouse	单克隆 Mono	1:1 000	GB122053
微管相关蛋白2 Microtubule associated protein 2 (MAP-2)	兔 Rabbit	多克隆 Poly	1:2 000	GB11128
神经元核 Neuronal nucleus (NeuN)	鼠 Mouse	单克隆 Mono	1:500	GB15138
钙结合蛋白 Soluble protein-100 (S-100)	兔 Rabbit	多克隆 Poly	1:500	GB11359
血管活性肠肽 Vasoactive intestinal peptide (VIP)	兔 Rabbit	多克隆 Poly	1:500	GB11279
CD99 Cluster of differentiation 99 (CD99)	兔 Rabbit	多克隆 Poly	1:100	GB111064

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一例猫鼻神经内分泌恶性肿瘤的病理和免疫组化分析

Pathological and Immunohistochemical Analysis of A Case of Feline Nasal Malignant Neuroendocrine Neoplasm

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