基于蛋白质组学解析鹅肥肝形成的脂质代谢调控机制

doi:10.11843/j.issn.0366-6964.2025.05.018

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (5): 2182-2193.doi: 10.11843/j.issn.0366-6964.2025.05.018

基于蛋白质组学解析鹅肥肝形成的脂质代谢调控机制

侯中一¹(), 王宝维¹^,²^,*(), 张名爱¹^,², 孔敏¹^,², 张晶³, 王秉翰⁴, 岳斌², 鲁秀¹, 凡文磊¹^,²^,*()

1. 青岛农业大学动物科技学院, 青岛 266109
2. 青岛农业大学优质水禽研究所, 青岛 266109
3. 青岛农业大学食品科学与工程学院, 青岛 266109
4. 青岛慧和生物科技有限公司, 青岛 266109

收稿日期:2024-09-24 出版日期:2025-05-23 发布日期:2025-05-27
通讯作者: 王宝维,凡文磊 E-mail:1429133886@qq.com;wangbw1959@qq.com;fanwenlei@qau.edu.cn
作者简介:侯中一(1999-)，男，山东济南人，硕士生，主要从事动物遗传与育种方向的研究，E-mail: 1429133886@qq.com
基金资助:
山东省重点研发计划(农业良种工程)项目(2024LZGC021)；山东省自然科学基金青年项目(ZR2020QC182)；财政部和农业农村部：国家现代农业产业技术体系资助项目(CARS-42-14)

The Regulation Mechanism of Lipid Metabolism in Foie Gras Formation Based on Proteomics Analysis

HOU Zhongyi¹(), WANG Baowei¹^,²^,*(), ZHANG Ming'ai¹^,², KONG Min¹^,², ZHANG Jing³, WANG Binghan⁴, YUE Bin², LU Xiu¹, FAN Wenlei¹^,²^,*()

1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
2. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China
3. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
4. Qingdao Huihe Biotechnology Co., Ltd., Qingdao 266109, China

Received:2024-09-24 Online:2025-05-23 Published:2025-05-27
Contact: WANG Baowei, FAN Wenlei E-mail:1429133886@qq.com;wangbw1959@qq.com;fanwenlei@qau.edu.cn

摘要/Abstract

摘要：

为探究鹅肥肝形成的分子机制，利用4D-DIA定量蛋白质组学技术分析了3个不同填饲阶段的朗德鹅肝脏样品。本研究选取同批次体况相近的70日龄朗德鹅，分别在填饲前期(7 d)、填饲中期(16 d)、填饲后期(25 d)随机选取3只鹅屠宰，采集肝大叶肝尖组织样，用于4D-DIA定量蛋白组学分析，共3组，每组3个生物学重复。结果：1)在3个不同填饲阶段的朗德鹅肝脏共鉴定出5 208个蛋白。2)通过主成分分析发现，3个填饲阶段鹅肝脏蛋白谱存在明显差异，填饲前期与中期鉴定出449个差异蛋白，中期与后期鉴定出303个差异蛋白。3)通过代谢通路分析发现，鹅肥肝形成过程中发生显著变化的通路主要涉及类固醇激素生物合成、脂肪细胞因子信号通路、氨基糖和核苷酸糖代谢、半胱氨酸和蛋氨酸代谢、花生四烯酸代谢、氨基酸的生物合成等，并利用荧光定量PCR技术筛选了关键差异基因ADIPOQ、PASK。填饲导致鹅肝脏蛋白组谱发生显著变化，与类固醇激素生物合成、脂肪细胞因子信号通路等相关的蛋白可能参与到了鹅肥肝形成的分子调控。

关键词: 鹅肥肝, 4D-DIA定量蛋白质组学, 脂质代谢, 差异蛋白, 分子调控机制

Abstract:

The study aimed to explore the molecular mechanism of foie gras formation, liver samples of Landes geese at 3 different feeding stages were analyzed by 4D-DIA quantitative proteomics. The 70-day-old Landes geese of the same batch with similar body condition were randomly selected and slaughtered at the early stage (7 d), middle stage (16 d) and final stage (25 d), 3 in each stage, respectively. The liver lobe and liver apex tissue samples were collected for 4D-DIA quantitative proteomic analysis. There were 3 groups with 3 biological replicates each. Result: 1) A total of 5 208 proteins were identified by comparison of the Landes geese liver at three different feeding stages. 2) Principal component analysis revealed significant differences in liver protein profiles across the 3 over-feeding stages, with 449 differential proteins identified in the comparison of early stage vs. middle stage, and 303 differential proteins identified in the comparison of middle stage vs. final stage. 3) Through pathway analysis of the differential metabolites, the enriched pathways were observed to be mainly involved in steroid hormone biosynthesis, ADIPOQ signaling pathway, amino sugar and nucleotide sugar metabolism, cysteine and methionine metabolism, arachidonic acid metabolism, and amino acid biosynthesis. The key differential genes ADIPOQ and PASK were identified based on RT-qPCR. The proteomic profile of liver of geese was significantly changed after over-feeding. The proteins related to steroid hormone biosynthesis and adipocytokine signaling pathway may be involved in the molecular regulation of foie gras formation.

Key words: foie gras, 4D-DIA quantitative proteomics, lipid metabolism, differential protein, molecular regulation mechanism

中图分类号:

S835.2

侯中一, 王宝维, 张名爱, 孔敏, 张晶, 王秉翰, 岳斌, 鲁秀, 凡文磊. 基于蛋白质组学解析鹅肥肝形成的脂质代谢调控机制[J]. 畜牧兽医学报, 2025, 56(5): 2182-2193.

HOU Zhongyi, WANG Baowei, ZHANG Ming'ai, KONG Min, ZHANG Jing, WANG Binghan, YUE Bin, LU Xiu, FAN Wenlei. The Regulation Mechanism of Lipid Metabolism in Foie Gras Formation Based on Proteomics Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2182-2193.

图/表 8

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基因 Gene	引物序列(5′→3′) Primer sequence
PASK	F: CGCGATGGCTGCTGAGG
	R: GACTCCTCGCCCATAGCTTC
ADIPOQ	F: ACCAGCCGCTAACAAGGG
	R: CTTGGTCGACGTTGTTGGTC
GAPDH	F: TCGGAGTCAACGGATTTGGC
	R: TTCTCAGCCTTGACTGTGCC

组别 Group	蛋白ID Protein ID	基因名 Gene name	蛋白功能描述 Protein function description	倍数变化 Fold change	P
下调 Down	A0A8B9DKQ2	CLDN20	Claudin	0.50	0.00
	A0A8B9DNV2	CD207	C-type lectin domain-containing protein	0.51	0.00
	A0A8B9DCX9	Romo1	Reactive oxygen species modulator 1	0.66	0.00
	A0A8B9DBW8	AMP1	AMP1 protein	0.15	0.00
	A0A8B9D2F8	Rv1747	ABC transporter domain-containing protein	0.48	0.00
	A0A8B9DI93	Cygb	Cytoglobin	0.60	0.00
	A0A8B9DKF8	PRUNE1	Prune exopolyphosphatase 1	0.65	0.00
	A0A8B9DPQ1	Siglec12	Ig-like domain-containing protein	0.60	0.00
	A0A8B9E544	ESP1	separase	0.50	0.00
	A0A8B9E3Q7	frvX	Aminopeptidase	0.66	0.00
上调 Up	A0A8B9E098	MARCHF5	S Mitochondrial fission regulator	1.88	0.00
	A0A8B9D9H9	Txlng	Taxilin gamma	1.57	0.00
	A0A8B9DSE6	TMED10	Transmembrane protein 263	2.27	0.00
	A0A8B9IND4	PAFAH2	Platelet-activating factor acetylhydrolase	2.82	0.00
	A0A8B9D8G3	LIAS	Lipoyl synthase	1.73	0.00
	A0A8B9DZ79	mid1ip1b	M1I1B protein	2.30	0.00
	A0A8B9EDG7	uppS	polycis-polyprenyl diphosphate synthase	3.10	0.00
	A0A8B9D5N3	UTP14A	U3 small nucleolar RNA-associated protein 14 homolog A	1.70	0.00
	A0A8B9E2N7	RB18B	RB18B protein	2.09	0.00
	A0A8B9DEC2	Cacybp	Calcyclin-binding protein	1.80	0.00

组别 Group	蛋白ID Protein ID	基因名 Gene name	蛋白功能描述 Protein function description	倍数变化 Fold change	P
下调Down	A0A8B9EM53		NADH: ubiquinone oxidoreductase core subunit V1	0.63	0.00
	A0A8B9E7E8	SC5D	Sterol-C5-desaturase	0.52	0.00
	A0A8B9ET89	Msed_0406	propionate--CoA ligase	0.54	0.00
	A0A8B9INS7	MT1H	Metallothionein	0.17	0.00
	A0A8B9DKF2	SEC61B	Protein transport protein Sec61 subunit beta	0.60	0.00
	A0A8B9D493	TMX3	thioredoxin-disulfide reductase	0.66	0.00
	A0A8B9EDT5	SYNE3	Spectrin repeat containing nuclear envelope family member 3	0.45	0.00
	A0A8B9EK56	PFKFB1	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 1	0.62	0.00
	A0A8B9E4B6	PASK	Protein kinase domain-containing protein	0.66	0.00
	A0A8B9INT0	TMEM41B	Transmembrane protein 177	0.35	0.00
上调 Up	A0A8B9D7K1	Plxnb2	Plexin B2	1.59	0.00
	A0A8B9D8Z5	Tacc1	Transforming acidic coiled-coil containing protein 2	2.05	0.00
	A0A8B9DGJ0	PDLIM4	PDZ and LIM domain 1	2.45	0.00
	A0A8B9DDN0	Urah	5-hydroxyisourate hydrolase	4.93	0.00
	A0A8B9DRG6	ADIPOQ	Adiponectin, C1Q and collagen domain containing	1.53	0.00
	A8B9DB57	CBX2	Chromobox 1	2.66	0.00
	A0A8B9DEL4	DEFB114	Beta-defensin 2	4.55	0.00
	B2ZR74	SSA1	Heat shock protein 70	1.61	0.00
	A0A8B9DD15	Cobl	Cordon-bleu WH2 repeat protein	1.98	0.00
	A0A8B9DMG4	ITSN1	Intersectin 1	1.52	0.00

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基于蛋白质组学解析鹅肥肝形成的脂质代谢调控机制

The Regulation Mechanism of Lipid Metabolism in Foie Gras Formation Based on Proteomics Analysis

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