干扰MAPK6基因对秦川牛成肌细胞分化的影响研究

doi:10.11843/j.issn.0366-6964.2025.11.011

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (11): 5475-5488.doi: 10.11843/j.issn.0366-6964.2025.11.011

干扰MAPK6基因对秦川牛成肌细胞分化的影响研究

李炳志¹^,²^,⁴(), 郭俊涛²^,³, 王建芳²^,³, 于胜晨²^,³, 潘月婷²^,³, 余横伟²^,³, 刘海兵²^,³, 张可²^,³, 成功²^,³, 田万强¹^,⁴^,*(), 昝林森²^,³^,*()

1. 陕西农林职业技术大学动物工程学院, 杨凌 712100
2. 西北农林科技大学动物科技学院, 杨凌 712100
3. 国家肉牛改良中心, 杨凌 712100
4. 反刍动物高效繁育技术陕西省高等学校重点实验室, 杨凌 712100

收稿日期:2025-03-25 出版日期:2025-11-23 发布日期:2025-11-27
通讯作者: 田万强,昝林森 E-mail:3640910@qq.com;twqiang2003@163.com;zanlinsen@163.com
作者简介:李炳志(1982-)，男，山东人，博士，主要从事动物遗传育种与繁殖研究，E-mail: 3640910@qq.com
基金资助:
国家重点研发计划(2023YFD1300100)；国家肉牛牦牛产业技术体系项目(CARS-37)；陕西省重点研发计划(2022ZDLNY01-01)；陕西省畜禽育种两链融合重点专项(2022GD-TSLD-46-0102)；陕西省教育厅重点科学研究计划项目(24JS060)

Study on the Effect of Interfering with MAPK6 Gene on the Differentiation of Qinchuan Cattle Myoblast Cells

LI Bingzhi¹^,²^,⁴(), GUO Juntao²^,³, WANG Jianfang²^,³, YU Shengchen²^,³, PAN Yueting²^,³, YU Hengwei²^,³, LIU Haibing²^,³, ZHANG Ke²^,³, CHENG Gong²^,³, TIAN Wanqiang¹^,⁴^,*(), ZAN Linsen²^,³^,*()

1. College of Animal Engineering, Shaanxi A&F Technology University, Yangling 712100, China
2. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
3. National Beef Cattle Improvement Center, Yangling 712100, China
4. Key Laboratory for Efficient Ruminant Breeding Technology of Higher Education Institutions in Shaanxi Province, Yangling 712100, China

Received:2025-03-25 Online:2025-11-23 Published:2025-11-27
Contact: TIAN Wanqiang, ZAN Linsen E-mail:3640910@qq.com;twqiang2003@163.com;zanlinsen@163.com

摘要/Abstract

摘要：

旨在探究干扰MAPK6(细胞外信号调节激酶3，ERK3)基因对秦川牛成肌细胞分化的影响及其作用机理。本研究通过qRT-PCR技术检测MAPK6基因在秦川牛不同组织中的表达谱。采用siRNA技术干扰MAPK6基因在秦川牛成肌细胞中的表达，通过qRT-PCR和Western blot检测其干扰效率及肌管分化相关基因的表达水平，并观察肌管表型变化。随后对其进行转录组测序，筛选差异表达基因并进行GO功能注释和KEGG通路富集分析。此外，对成肌分化相关通路进行了初步验证。结果表明，MAPK6在秦川牛背最长肌组织中特异性高表达。干扰MAPK6极显著促进了秦川牛成肌细胞的肌管分化融合(P < 0.01)，极显著增加了成肌分化相关基因MEF2C、MYH7的表达(P < 0.01)及MEF2C、MYL2的蛋白表达(P < 0.01)，显著增加成肌MYH7的蛋白表达(P < 0.05)。转录组结果发现，与对照组相比，干扰MAPK6基因共有3 224个基因差异表达，其中上调1 707个，下调1 517个。KEGG富集结果显示，差异基因主要富集在PI3K-AKT、MAPK信号通路。MAPK6基因的干扰会显著降低MAPK信号通路中ERK1/2、p-ERK1/2蛋白的表达(P < 0.01)。本研究初步阐明了MAPK6基因在牛肌肉生成中的作用和机理，为提高牛肉品质和指导肉牛分子育种策略奠定基础。

关键词: 秦川牛, 骨骼肌, MAPK6基因, 成肌细胞分化

Abstract:

The aim of this study was to investigate the effect of interfering with MAPK6 (extracellular signal regulated kinase 3, ERK3) gene on the differentiation of Qinchuan cattle myoblasts and its action mechanism. The expression profile of the MAPK6 gene in different tissues of Qinchuan cattle was detected using qRT-PCR technology. siRNA technology was used to interfere with the expression of MAPK6 gene in Qinchuan cattle myoblasts. The interference efficiency and expression levels of myotube differentiation related genes were detected by qRT-PCR and Western blot, and changes in myotube phenotype were observed. Subsequently, transcriptome sequencing was performed to screen differentially expressed genes, followed by GO functional annotation and KEGG pathway enrichment analysis. Additionally, preliminary validation of myogenic differentiation-related pathways was conducted. The results indicated that MAPK6 was highly expressed in the longissimus dorsi muscle tissue of Qinchuan cattle. Interfering with MAPK6 significantly promoted myotube differentiation and fusion in Qinchuan cattle myoblasts (P < 0.01), significantly increased the expression of myogenic differentiation-related genes MEF2C and MYH7 (P < 0.01) and the protein expression of MEF2C and MYL2 (P < 0.01), and significantly increased the protein expression of MYH7 (P < 0.05). The transcriptome results showed that, compared with the control group, there were 3 224 differentially expressed genes after interfering with MAPK6, of which 1 707 were upregulated and 1 517 were downregulated. The KEGG enrichment results showed that differential genes were mainly enriched in the PI3K-AKT and MAPK signaling pathways. Interfering with the MAPK6 gene could significantly reduces the expression of ERK1/2 and p-ERK1/2 proteins (P < 0.01) in the MAPK signaling pathway. This study preliminarily elucidates the role and mechanism of MAPK6 gene in beef muscle production, laying a foundation for improving beef quality and guiding molecular breeding strategies in beef cattle.

Key words: Qinchuan cattle, skeletal muscles, MAPK6 gene, myoblast differentiation

中图分类号:

S823.2

李炳志, 郭俊涛, 王建芳, 于胜晨, 潘月婷, 余横伟, 刘海兵, 张可, 成功, 田万强, 昝林森. 干扰MAPK6基因对秦川牛成肌细胞分化的影响研究[J]. 畜牧兽医学报, 2025, 56(11): 5475-5488.

LI Bingzhi, GUO Juntao, WANG Jianfang, YU Shengchen, PAN Yueting, YU Hengwei, LIU Haibing, ZHANG Ke, CHENG Gong, TIAN Wanqiang, ZAN Linsen. Study on the Effect of Interfering with MAPK6 Gene on the Differentiation of Qinchuan Cattle Myoblast Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5475-5488.

图/表 10

表 1

图 1

图 2

图 3

表 2

表 3

图 4

图 5

图 6

图 7

参考文献 36

1	付玉, 张博, 凌遥, 等. 骨骼肌生长发育过程及调控研究现状[J]. 中国畜牧兽医, 2021, 48 (10): 3565- 3574.
	FU Y , ZHANG B , LING Y , et al. Reviews on process and regulation of skeletal muscle growth and development[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48 (10): 3565- 3574.
2	WANG Y P , ZHANG Z J , ZHANG Y Q , et al. Regulatory role of TEX10 gene in proliferation differentiation and apoptosis of bovine myoblasts[J]. Int J Biochem Cell Biology, 2025, 182-183, 106771. doi: 10.1016/j.biocel.2025.106771
3	凌笑笑, 唐朋, 梁春年, 等. miRNAs对骨骼肌调控的研究进展[J]. 中国畜牧兽医, 2018, 45 (6): 1486- 1492.
	LING X X , TANG P , LIANG C N , et al. Research progress on miRNAs in skeletal muscle regulation[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45 (6): 1486- 1492.
4	LI L , ZHANG Z H , XU H D , et al. Chicken CircZNF609 encodes a protein induced by IRES-like region that inhibits the proliferation and promotes the differentiation of myoblasts[J]. Poul Sci, 2025, 104 (8): 105339. doi: 10.1016/j.psj.2025.105339
5	WEN X M , JIAO L D , TAN H . MAPK/ERK pathway as a central regulator in vertebrate organ regeneration[J]. Int J Mol Sci, 2022, 23 (3): 1464. doi: 10.3390/ijms23031464
6	TURGEON B , SABA-EL-LEIL M K , MELOCHE S . Cloning and characterization of mouse extracellular-signal-regulated protein kinase 3 as a unique gene product of 100 kDa[J]. Biochem J, 2000, 346 (Pt 1): 169- 175.
7	SOULEZ M , SABA-EL-LEIL M K , TURGEON B , et al. Reevaluation of the role of extracellular signal-regulated kinase 3 in perinatal survival and postnatal growth using new genetically engineered mouse models[J]. Mol Cell Biol, 2019, 39 (6): e00527- 18.
8	SOULEZ M , TANGUAY PL , DO F , et al. ERK3-MK5 signaling regulates myogenic differentiation and muscle regeneration by promoting FoxO3 degradation[J]. J Cell Physiol, 2022, 237 (4): 2271- 2287. doi: 10.1002/jcp.30695
9	COULOMBE P , RODIER G , PELLETIER S , et al. Rapid turnover of extracellular signal-regulated kinase 3 by the ubiquitin-proteasome pathway defines a novel paradigm of mitogen-activated protein kinase regulation during cellular differentiation[J]. Mol Cell Biol, 2003, 23 (13): 4542- 4558. doi: 10.1128/MCB.23.13.4542-4558.2003
10	LI B Z , WANG J F , RAZA S H A , et al. MAPK family genes' influences on myogenesis in cattle: genome-wide analysis and identification[J]. Res Vet Sci, 2023, 159, 198- 212. doi: 10.1016/j.rvsc.2023.04.024
11	龚宇轩, 黑伟, 鲍武, 等. TMEM182基因调控猪骨骼肌卫星细胞成肌分化的研究[J]. 畜牧兽医学报, 2025, 56 (4): 1676- 1688. doi: 10.11843/j.issn.0366-6964.2025.04.017
	GONG Y X , HEI W , BAO W , et al. Study on the regulation of myogenie differentiation of porcine skeletal muscle satellite cells by gene TMEM182[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56 (4): 1676- 1688. doi: 10.11843/j.issn.0366-6964.2025.04.017
12	赵晓, 莫德林, 张悦, 等. 猪的骨骼肌生长发育研究进展[J]. 生命科学, 2011, 23 (1): 37- 44.
	ZHAO X , MO D L , ZHANG Y , et al. Progress in research on skeletal muscle growth and development in swine[J]. Chinese Bulletin of Life Sciences, 2011, 23 (1): 37- 44.
13	JEONG S Y , CHOI J H , ALLEN P D , et al. Immature skeletal myotubes are an effective source for improving the terminal differentiation of skeletal muscle[J]. Cells, 2024, 13 (24): 2136. doi: 10.3390/cells13242136
14	PIASECKA A , SEKRECKI M , SZCZES'NIAK M W , et al. MEF2C shapes the microtranscriptome during differentiation of skeletal muscles[J]. Sci Rep, 2021, 11 (1): 3476. doi: 10.1038/s41598-021-82706-2
15	DONG C , YANG X Z , ZHANG C Y , et al. Myocyte enhancer factor 2C and its directly-interacting proteins: A review[J]. Progress Biophys MolBiol, 2017, 126, 22- 30. doi: 10.1016/j.pbiomolbio.2017.02.002
16	BHARATHY N , LING B M , TANEJA R . Epigenetic regulation of skeletal muscle development and differentiation[J]. Subcell Biochem, 2013, 61, 139- 150.
17	YANG X R , NING Y , ABBAS RAZA S H , et al. MEF2C expression is regulated by the post-transcriptional activation of the METTL3-m(6)A-YTHDF1 axis in myoblast differentiation[J]. Front Vet Sci, 2022, 9, 900924. doi: 10.3389/fvets.2022.900924
18	刘瑞莉, 吴磊, 袁玮, 等. MYL2基因在肌肉生长过程中的研究[J]. 黑龙江畜牧兽医, 2018 (3): 10-14+252.
	LIU R L , WU L , YUAN W , et al. The study on the function of MYL2 gene in muscle growth[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018 (3): 10-14+252.
19	CIECIERSKA A , MOTYL T , SADKOWSKI T . Transcriptomic profile of primary culture of skeletal muscle cells isolated from semitendinosus muscle of beef and dairy bulls[J]. Int J Mol Sci, 2020, 21 (13): 4794. doi: 10.3390/ijms21134794
20	WANG H J , DOU M M , LI J , et al. Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation[J]. In Vitro Cell Dev Biol Anim, 2022, 58 (9): 798- 809. doi: 10.1007/s11626-022-00721-7
21	TAO L J , HUANG W X , LI Z Y , et al. Transcriptome analysis of differentially expressed genes and molecular pathways involved in C2C12 cells myogenic differentiation[J]. Mol Biotechnol, 2025, 67 (9): 3640- 3655. doi: 10.1007/s12033-024-01259-7
22	ENDO T . Postnatal skeletal muscle myogenesis governed by signal transduction networks: MAPKs and PI3K-Akt control multiple steps[J]. Biochem Biophys Res Commun, 2023, 682, 223- 243. doi: 10.1016/j.bbrc.2023.09.048
23	陈博, 邓强, 李中锋, 等. Hippo信号通路成骨、成肌分化功能研究进展[J]. 中国骨质疏松杂志, 2023, 29 (10): 1538- 1543.
	CHEN B , DENG Q , LI Z F , et al. Research progress of Hippo signaling pathway in osteogenic and myogenic differentiation[J]. Chinese Journal of Osteoporosis, 2023, 29 (10): 1538- 1543.
24	程春芳, 万娟, 丁恺志, 等. 成肌细胞增殖与分化及其调控机制[J]. 中国组织工程研究, 2023, 27 (14): 2200- 2206.
	CHENG C F , WAN J , DING K Z , et al. Regulatory mechanism of myoblast proliferation and differentiation[J]. Chinese Journal ofTissue Engineering Research, 2023, 27 (14): 2200- 2206.
25	SEGALÉS J , PERDIGUERO E , MUÑOZ-CÁNOVES P . Regulation of muscle stem cell functions: a focus on the p38 MAPK signaling pathway[J]. Front Cell Dev Biol, 2016, 4, 91.
26	党彩霞, 朱袁婕, 包雅婷, 等. p38-MAPK信号通路对骨骼肌再生的影响研究进展[J]. 江西科技师范大学学报, 2023 (6): 109- 113.
	DANG C X , ZHU Y J , BAO Y T , et al. Research progress on the effect of p38 MAPK signaling pathway on skeletal muscle regeneration[J]. Journal of Jiangxi Science & Technology Normal University, 2023 (6): 109- 113.
27	郑丽蓉, 许艳华, 曾卫卫, 等. 细胞膜去极化通过激活MAPK信号通路促进原代肌母细胞增殖[J]. 南京师大学报(自然科学版), 2020, 43 (1): 100- 106.
	ZHENG L R , XU Y H , ZENG W W , et al. Plasma membrane depolarization promotes primary myoblast proliferation through activation of MAPK signaling pathway[J]. Journal of Nanjing Normal University(Natural Science Edition), 2020, 43 (1): 100- 106.
28	HU Y , FU J H , LIU X Y , et al. ERK1/2 signaling pathway activated by EGF eromotes proliferation, transdifferentiation, and migration of cultured primary newborn rat lung fibroblasts[J]. Biomed Res Int, 2020, 2020, 7176169. doi: 10.1155/2020/7176169
29	MARTIN-VEGA A , COBB M H . ERK1/2-MAPK signaling: metabolic, organellar, and cytoskeletal interactions[J]. Curr Opin Cell Biol, 2025, 95, 102526. doi: 10.1016/j.ceb.2025.102526
30	EIGLER T , ZARFATI G , AMZALLAG E , et al. ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-tomyotube fusion[J]. Dev Cell, 2021, 56 (24): 3349- 3363. doi: 10.1016/j.devcel.2021.11.022
31	FU X J , MATSUI T , FUNABA M . Enhancement of vitamin C-induced myogenesis by inhibition of extracellular signal-regulated kinase (ERK) 1/2 pathway[J]. Biochem Biophys Res Commun, 2022, 612, 57- 62. doi: 10.1016/j.bbrc.2022.04.103
32	LIU H , LEE S M , JOUNG H . 2-D08 treatment regulates C2C12 myoblast proliferation and differentiation via the Erk1/2 and proteasome signaling pathways[J]. J Muscle Res Cell Motil, 2021, 42 (2): 193- 202. doi: 10.1007/s10974-021-09605-x
33	BENNETT A M , TONKS N K . Regulation of distinct stages of skeletal muscle differentiation by mitogen-activated protein kinases[J]. Science, 1997, 278 (5341): 1288- 1291. doi: 10.1126/science.278.5341.1288
34	CHEN S J , YUE J , ZHANG J X , et al. Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway[J]. Stem Cell Res Ther, 2019, 10 (1): 70. doi: 10.1186/s13287-019-1160-x
35	LIU S F , GAO F , WEN L , et al. Osteocalcin induces proliferation via positive activation of the PI3K/Akt, P38 MAPK pathways and promotes differentiation through activation of the GPRC6A-ERK1/2 pathway in C2C12 myoblast cells[J]. Cell Physiol Biochem, 2017, 43 (3): 1100- 1112. doi: 10.1159/000481752
36	KUPPUSAMY P , SOUNDHARRAJAN I , KIM D H , et al. 4-hydroxy-3-methoxy cinnamic acid accelerate myoblasts differentiation on C2C12 mouse skeletal muscle cells via AKT and ERK 1/2 activation[J]. Phytomedicine, 2019, 60, 152873. doi: 10.1016/j.phymed.2019.152873

基因 Gene	GenBank号 GenBank number	引物 Primer	引物序列(5′→3′) Primer sequence	产物长度/bp Product length
GAPDH	NM_001034034.2	GAPDH-F	AGTTCAACGGCACAGTCAAGG	124
GAPDH	NM_001034034.2	GAPDH-R	ACCACATACTCAGCACCAGCA	124
MAPK6	XM_059890855.1	MAPK6-F	ACTTGGTGCTGAAGATAGGTGA	206
MAPK6	XM_059890855.1	MAPK6-R	AAAGAGGGTTTTACCAGTCAGC	206
MEF2C	XM_059888100.1	MEF2C-F	GCACCAACAAGCTGTTCCAG	117
MEF2C	XM_059888100.1	MEF2C-R	GAACAGCCTCCACGATGTCT	117
MYH7	XM_024997290.2	MYH7-F	TCCAGTCTTCTCTCCTTGCTG	187
MYH7	XM_024997290.2	MYH7-R	AGGTCGAAAGGCCTGGTCTG	187
MYL2	NM_001035025.2	MYL2-F	CTTTCCACCATGTCACCTAAGA	114
MYL2	NM_001035025.2	MYL2-R	GATGGTGAAGGCCTCCTTAAA	114
CEND1	NM_001080222.1	CEND1-F	GAGCAGCAGCGCCCATA	125
CEND1	NM_001080222.1	CEND1-R	CTTCCCTCTGGACTCCATGC	125
SORD	NM_001037320.1	SORD-F	CTGGCAGCATGGTCGAATTG	102
SORD	NM_001037320.1	SORD-R	TGCCTGACCAATGATCCCAC	102
WASF3	XM_005213794.5	WASF3-F	CCAGGTTATCACAGAGCGCA	102
WASF3	XM_005213794.5	WASF3-R	CCGGGTACGAGTAATCCGTG	102
VPS50	NM_001105413.2	VPS50-F	ATGAGGGTCGTGCCTTGATG	162
VPS50	NM_001105413.2	VPS50-R	TGTGCTCTTTGATCCAGCGT	162
ZFP90	XM_059877474.1	ZFP90-F	CGAGTGAAGACGGCACAGAA	85
ZFP90	XM_059877474.1	ZFP90-R	CCAGGGACAGGGTTGACTTC	85
CCNG1	NM_001013364.1	CCNG1-F	GCTGTGAAGCCTCAAAACATC	170
CCNG1	NM_001013364.1	CCNG1-R	TCCAGGCTTGTCATTTGCATT	170
NR1D2	XM_059882241.1	NR1D2-F	GTCTAATTACCTGGTTTCCCCC	127
NR1D2	XM_059882241.1	NR1D2-R	GACGCGGACTGGAAGCTATT	127
GCSH	NM_174844.1	GCSH-F	TATTGAGGAGTGAAACTGGAACCC	188
GCSH	NM_174844.1	GCSH-R	AGATGCAGTGTTTATCTGAAGTCAT	188
ABCB10	XM_024986813.2	ABCB10-F	CTCATGCAGACGTCAGGTCA	107
ABCB10	XM_024986813.2	ABCB10-R	TCTCCTGTGCGTGTCTTGTC	107

样品 Sample	原始数据Raw data		过滤数据Clean data		有效比对率/% Valid ratio (reads)	Q20%	Q30%	GC含量/% GC content
样品 Sample	读数 Read	碱基/G Base	读数 Read	碱基/G Base	有效比对率/% Valid ratio (reads)	Q20%	Q30%	GC含量/% GC content
MAPK6S1	40 273 634	6.04	38 754 750	5.81	96.23	99.93	98.04	50.50
MAPK6S2	40 890 852	6.13	39 414 348	5.91	96.39	99.94	97.97	50.50
MAPK6S3	40 659 854	6.10	39 237 220	5.89	96.50	99.94	98.04	49.50
SINC1	39 899 744	5.98	38 249 602	5.74	95.86	99.93	97.97	50.50
SINC2	38 885 912	5.83	37 072 804	5.56	95.34	99.92	97.89	51.50
SINC3	39 628 850	5.94	38 159 344	5.70	96.29	99.93	97.97	51.50

样品 Sample	过滤数据 Clean reads	比对读数 Mapped reads	唯一比对读数 Unique mapped reads	多比对读数 Multi mapped reads
MAPK6S1	38 754 750	36 246 759(93.53%)	35 413 870(91.38%)	832 889(2.15%)
MAPK6S2	39 414 348	36 937 794(93.72%)	36 105 382(91.60%)	832 412(2.11%)
MAPK6S3	39 237 220	36 907 942(94.06%)	36 079 312(91.95%)	828 630(2.11%)
SINC1	38 249 602	35 839 948(93.70%)	34 934 213(91.33%)	905 735(2.37%)
SINC2	37 072 804	34 373 945(92.72%)	33 513 528(90.40%)	860 417(2.32%)
SINC3	38 159 344	35 644 691(93.41%)	34 805 597(91.21%)	839 094(2.20%)

[1]	胡金玲, 钟奇祺, 黄程, 雷明刚. AKR1B1介导AMPK/mTOR/S6通路调控猪骨骼肌卫星细胞增殖和分化[J]. 畜牧兽医学报, 2025, 56(8): 3722-3733.
[2]	吴斯林, 杨本顺, 叶苗苗, 梁恩堂, 李付强, 马伟东, 昝林森, 赵春平, 杨武才. 木犀草素对秦川牛精子冷冻保存的影响[J]. 畜牧兽医学报, 2025, 56(7): 3244-3251.
[3]	刘雨蒙, 高星, 赵雅丽, 曹迪, 芒来, 张心壮. 硒多糖缓解马骨骼肌卫星细胞氧化损伤作用的研究[J]. 畜牧兽医学报, 2025, 56(7): 3357-3367.
[4]	石闪闪, 万琼飞, 许赢心, 王秋硕, 张林林, 郭益文, 胡德宝, 郭宏, 丁向彬, 李新. 牛骨骼肌不同发育阶段miRNA测序及生物信息学分析[J]. 畜牧兽医学报, 2025, 56(6): 2701-2710.
[5]	龚宇轩, 黑伟, 鲍武, 陈佳仪, 李萌, 郭晓红, 李步高. TMEM182基因调控猪骨骼肌卫星细胞成肌分化的研究[J]. 畜牧兽医学报, 2025, 56(4): 1676-1688.
[6]	李远方, 张鸿源, 李鸿泰, 李智, 魏千然, 王亚东, 李国喜, 王丹丹, 刘翘铭. 胚蛋给养核黄素对鸡骨骼肌发育的影响[J]. 畜牧兽医学报, 2025, 56(3): 1159-1169.
[7]	张正雨, 杨培鸿, 郭宏, 李新, 张林林, 郭益文, 胡德宝, 丁向彬. 去乙酰化酶Sirt1对牛骨骼肌卫星细胞增殖和分化的影响[J]. 畜牧兽医学报, 2025, 56(2): 603-610.
[8]	赵刚奎, 高海旭, 尹思琦, 孙红红, 辛怡然, 昝林森, 赵春平. SFRP4基因对牛前体脂肪细胞分化作用的影响[J]. 畜牧兽医学报, 2025, 56(2): 611-620.
[9]	张帅, 徐景, 杨培鸿, 郭益文, 胡德宝, 李新, 丁向彬, 郭宏, 张林林. PFN1-PTEN通过调控PI3K/AKT/mTOR抑制牛骨骼肌卫星细胞分化[J]. 畜牧兽医学报, 2025, 56(11): 5489-5501.
[10]	何思琦, 陈倩, 蒋琳, 马月辉, 周胜花, 赵倩君. 基于转录组测序分析METTL14对绵羊骨骼肌卫星细胞成肌分化的影响[J]. 畜牧兽医学报, 2025, 56(10): 4925-4937.
[11]	冯铭, 伊旭东, 庞卫军. 肠道微生物通过骨骼肌纤维类型、肌内脂肪含量和骨骼肌代谢调控猪肉质研究进展[J]. 畜牧兽医学报, 2024, 55(6): 2304-2312.
[12]	刘媛, 李溪月, 张维娅. MMP14调控骨骼肌卫星细胞分化的分子机制研究[J]. 畜牧兽医学报, 2024, 55(4): 1592-1604.
[13]	梁淑怡, 李凡, 江青艳, 王松波. 脯氨酸羟化酶(PHDs)对动物骨骼肌发育和脂肪沉积的调控作用及其机制[J]. 畜牧兽医学报, 2024, 55(3): 867-873.
[14]	吴丹妮, 谢遇春, 秦箐, 张崇妍, 徐晓龙, 赵丹, 兰茗熙, 杨继, 徐松松, 刘志红. 畜禽肌纤维发育相关细胞种类及鉴定方法的研究进展[J]. 畜牧兽医学报, 2024, 55(12): 5325-5339.
[15]	曹官从, 马露, 任灵芝, 李杨, 史新娥, 杨公社, 李晓. 基于单细胞测序技术探讨动物骨骼肌卫星细胞与生态位细胞之间的“对话”[J]. 畜牧兽医学报, 2024, 55(12): 5340-5348.

干扰MAPK6基因对秦川牛成肌细胞分化的影响研究

Study on the Effect of Interfering with MAPK6 Gene on the Differentiation of Qinchuan Cattle Myoblast Cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价