非洲猪瘟病毒结构蛋白与宿主蛋白相互作用研究进展

doi:10.11843/j.issn.0366-6964.2025.01.009

摘要/Abstract

摘要：

非洲猪瘟(African swine fever，ASF)是由非洲猪瘟病毒(African swine fever virus，ASFV)感染所引起的一种家猪和各种野猪急性出血的传染性疾病，由于高发病率和高致死性的特性对全球家猪养殖产业造成严重的经济损失。ASFV编码蛋白高达150多种，而ASFV结构蛋白作为病毒粒子的主要组成部分，在协助病毒吸附入侵宿主细胞、促进子代病毒颗粒复制、组装以及释放等过程发挥着重要作用。研究表明，病毒结构蛋白可通过与宿主蛋白的相互作用，促进病毒入侵、增殖、影响病毒毒力以及拮抗宿主免疫反应等。因此，本文通过概述ASFV结构蛋白与宿主蛋白的相互作用及其机制，为研究ASFV的致病机制以及ASF的防治提供参考。

关键词: 非洲猪瘟病毒, 结构蛋白, 宿主蛋白, 蛋白相互作用

Abstract:

African swine fever (ASF) is a highly contagious disease characterized by severe bleeding in both domestic pigs and various wild boars caused by infection with the African swine fever virus (ASFV). The global pig breeding industry has suffered significant economic losses due to the high morbidity and mortality rates associated with ASF. ASFV contains over 150 different proteins, with its structural proteins playing a crucial role in facilitating the virus's attachment to and entry into host cells, as well as in the replication, assembly, and release of new viral particles. Research has demonstrated that these viral structural proteins can enhance virus invasion and replication, impact viral virulence, and counteract the host immune response through interactions with host proteins. This review summarizes the interactions between ASFV structural proteins and host proteins, along with their underlying mechanisms, in order to contribute to the understanding of ASFV pathogenesis and the development of strategies for its prevention and treatment.

Key words: African swine fever virus (ASFV), structural protein, host protein, protein-protein interaction

中图分类号:

S852.659.1

张素, 孙丽芳, 李兰兰, 吴琳娇, 陈磊清, 吴允昆. 非洲猪瘟病毒结构蛋白与宿主蛋白相互作用研究进展[J]. 畜牧兽医学报, 2025, 56(1): 95-106.

ZHANG Su, SUN Lifang, LI Lanlan, WU Linjiao, CHEN Leiqing, WU Yunkun. Research Progress on the Interactions of African Swine Fever Virus Structural Proteins with Host Proteins[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 95-106.

图/表 3

图 1

图 2

表 1

ASFV结构蛋白与宿主蛋白相互作用"

病毒蛋白 Viral protein	蛋白类别 Protein categories	编码基因 Coding gene	互作宿主蛋白 Interacting host proteins	互作机制 Interaction mechanism	参考文献 Reference
P30	内囊膜蛋白 Inner envelopeprotein	CP204L	hn-RNP-K、DAB2等	影响正常核质运输；吸附宿主细胞、释放成熟病毒粒子；激活MAPK通路；抑制STATI磷酸化等 Affects normal nucleoplasmic transport; adsorbs host cells, releases mature viral particles; activates MAPK pathway; inhibits STATI phosphorylation, etc.	[27, 38]
CD2V	外囊膜蛋白 Outer envelopeprotein	EP402R	AP-1、CSF2RA、HIP-55	利用网格蛋白进行迁移；影响细胞内吞或蛋白质运输；激活JAK2-STAT3通路 Migration using lattice proteins; affects endocytosis or protein transport; activates JAK2-STAT3 pathway	[23, 49, 57]
P54	内囊膜蛋白 Inner envelopeprotein	E183L	DLC8	利用微管动力蛋白运输，激活caspase-9和caspase-3，诱导细胞凋亡 Induction of apoptosis by activation of caspase-9 and caspase-3 using microtubule dynamin transport	[61]
P72	衣壳蛋白 Capsid protein	B646L	OAS1	泛素化P72蛋白；抑制avSG的产生 Ubiquitination of P72 protein; inhibition of avSG production	[65]
P17	内囊膜蛋白 Inner envelope protein	D117L	STING、TOMM70	负调控cGAS-STING信号通路；促使线粒体自噬 Negative regulation of the cGAS-STING signaling pathway; Promotes mitochondrial autophagy	[67-68]
P14.5	衣壳蛋白 Capsid protein	E120R	IRF3、Kinesin等	干扰IRF3的磷酸化和Ⅰ型干扰素的生成；介导成熟的病毒粒子离开病毒工厂进入胞浆膜 Interferes with IRF3 phosphorylation and type Ⅰ interferon production; mediates the exit of mature viral particles from the viral factory into the plasma membrane	[69-70]
P11.5	衣壳蛋白 Capsid protein	A137R	TANK结合激酶	降解TANK，阻断INF-β的产生 Degradation of TANK and blocking of INF-β production	[72]

表 1

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