14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析

doi:10.11843/j.issn.0366-6964.2021.028

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (1): 262-267.doi: 10.11843/j.issn.0366-6964.2021.028

14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析

李少晗^1,2, 由欣月^1,2, 范君文³, 徐一⁴, 郝雲峰^1,2, 崔尚金^1,2, 秦彤^1,2*

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 兽用药物与兽医生物技术北京科学观测站, 北京 100193;
3. 北京市动物疫病预防控制中心, 北京 102629;
4. 中国动物疾病预防控制中心, 北京 100125

收稿日期:2020-06-22 出版日期:2021-01-23 发布日期:2021-01-19
通讯作者: 秦彤,主要从事动物传染病病原学与流行病学研究,E-mail:qintong@caas.cn
作者简介:李少晗(1996-),女,河北邢台人,硕士,主要从事动物传染病病原学与流行病学研究,E-mail:1037017995@qq.com
基金资助:
中国农业科学院科技创新工程项目（ASTIP-IAS15）

Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing

LI Shaohan^1,2, YOU Xinyue^1,2, FAN Junwen³, XU Yi⁴, HAO Yunfeng^1,2, CUI Shangjin^1,2, QIN Tong^1,2*

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Scientific Observing and Experiment Station of Veterinary Drugs and Diagnostic Technology of Ministry of Agriculture, Beijing 100193, China;
3. Beijing Animal Disease Prevention and Control Center, Beijing 102629, China;
4. China Animal Diseases Control Center, Beijing 100125, China

Received:2020-06-22 Online:2021-01-23 Published:2021-01-19

摘要/Abstract

摘要： 从北京地区疑似细小病毒感染的犬粪拭子中成功分离鉴定出14株犬细小病毒（CPV）毒株，并对其完整的VP2和NS1基因进行了序列分析。结果表明，鉴定到的14株CPV毒株中，7株为New CPV-2a型，7株为CPV-2c型。此外，NS1的19、33、293、588、624和656位氨基酸以及VP2的13、574位氨基酸为新鉴定的氨基酸突变位点。基于VP2、NS1基因的系统进化分析表明，大部分的New CPV-2a型和CPV-2c型毒株与广西南宁和吉林长春地区的分离毒株亲缘关系密切，说明本次分离毒株与广西或吉林地区分离毒株具有相同的起源。本研究为更好地开展犬细小病毒流行病学调查提供了有益借鉴，也为深入研究犬细小病毒变异和传播的分子机制奠定了基础。

关键词: 犬细小病毒, 分离鉴定, VP2基因, NS1基因, 进化分析

Abstract: Fourteen strains of canine parvovirus (CPV) were successfully isolated and identified from feces swabs of dogs which were suspected to be infected CPV in Beijing, and their complete NS1 and VP2 genes were sequenced and analyzed. Among the 14 strains identified, the results showed that 7 strains were New CPV-2a and 7 strains were of CPV-2c. Besides, some new amino acid mutation sites were identified (amino acids 19, 33, 293, 588, 624 and 656 of the NS1 and amino acids 13 and 574 of the VP2). Phylogenetic analysis based on the VP2 and NS1 genes indicated that most of the New CPV-2a and CPV-2c strains were closely related to the isolated strains in Nanning, Guangxi, and Changchun, Jilin, indicating that the isolated strains had the same origin as the isolated strains in Guangxi or Jilin. This study provides a useful reference for better epidemiological investigation of canine parvovirus, and also builds a foundation for the in-depth study of the molecular mechanism of canine parvovirus variation and transmission.

Key words: canine parvovirus, isolation and identification, VP2 gene, NS1 gene, evolutionary analysis

中图分类号:

S852.659.2

李少晗, 由欣月, 范君文, 徐一, 郝雲峰, 崔尚金, 秦彤. 14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析[J]. 畜牧兽医学报, 2021, 52(1): 262-267.

LI Shaohan, YOU Xinyue, FAN Junwen, XU Yi, HAO Yunfeng, CUI Shangjin, QIN Tong. Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 262-267.

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14株北京地区犬细小病毒分离毒株的VP2、NS1基因序列分析

Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing

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