1例肉鸡肝恶性黑色素瘤的病理学诊断

doi:10.11843/j.issn.0366-6964.2021.029

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (1): 268-272.doi: 10.11843/j.issn.0366-6964.2021.029

• 研究简报 • 上一篇

1例肉鸡肝恶性黑色素瘤的病理学诊断

杨腊梅, 张婷, 张鼎*

山西农业大学动物医学学院, 太谷 030801

收稿日期:2020-05-15 出版日期:2021-01-23 发布日期:2021-01-19
通讯作者: 张鼎,主要从事动物中毒病、疫病的发生与免疫研究,E-mail:zdlucky2014@sina.com
作者简介:杨腊梅(1998-),女,山西长治人,学士,主要从事动物疾病发生机制研究,E-mail:yanglamei19@mails.ucas.ac.cn
基金资助:
山西省高等学校大学生创新创业训练项目（2018105）；山西省留学回国人员科技活动择优资助项目；山西省高等学校科技创新项目（2020L0150）

Pathological Diagnosis of a Case of Liver Malignant Melanoma in Broiler Chicken

YANG Lamei, ZHANG Ting, ZHANG Ding*

College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China

Received:2020-05-15 Online:2021-01-23 Published:2021-01-19

摘要/Abstract

摘要： 本研究旨在对临床上发现的1例肉鸡肝黑色素瘤进行病理学诊断。1羽25日龄AA肉鸡精神沉郁、极度消瘦，剖检发现其整个肝呈黑色。HE染色结果显示，病变肝内遍布大小不一、细胞核深染、细胞质内有大量黑色素颗粒沉积的异常细胞团。黑色素瘤诊断标志蛋白SOX 10和HMB-45抗体免疫组化染色均呈阳性。结合病理学检测和免疫组化结果，判定该病鸡肝患有恶性黑色素瘤。试验对病变肝进行胆色素染色和普鲁士蓝染色，结果均呈阴性，提示病变肝并非由胆红素或含铁血黄素淤积所致。综上表明，此例病鸡患有严重的肝恶性黑色素瘤，试验结果对禽类黑色素瘤的诊断与研究有参考价值。

关键词: 肉鸡, 肝, 黑色素瘤, 病理学诊断

Abstract: This research aimed to diagnose a case of liver melanoma that happened in a broiler chicken. A 25-day-old broiler chicken was observed depressed and extremely thin, and the whole liver was black at autopsy. Hematoxylin and eosin (HE) staining results show a large amount of abnormal cells with different sizes, hyperchromatic nuclei and severe melanin granules deposition in the diseased liver. Immunohistochemistry of the liver incubated with both SOX 10 and HMB-45 antibody show positive results. Based on the results of the pathological and immunohistochemical examination, the broiler chicken was diagnosed with serious liver malignant melanoma. Results of both bile pigment and Prussian blue staining were negative, suggesting that the liver lesion was not caused by bilirubin or hemosiderin deposition. In summary, based on the results of differential diagnosis, the broiler chicken was diagnosed with serious liver malignant melanoma. Results of the present study help to provide reference for the diagnosis of avian melanoma in poultry.

Key words: broiler chicken, liver, melanoma, pathological diagnosis

中图分类号:

S857.4

杨腊梅, 张婷, 张鼎. 1例肉鸡肝恶性黑色素瘤的病理学诊断[J]. 畜牧兽医学报, 2021, 52(1): 268-272.

YANG Lamei, ZHANG Ting, ZHANG Ding. Pathological Diagnosis of a Case of Liver Malignant Melanoma in Broiler Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 268-272.

参考文献 14

[1]	BADERCA F,VINCZE D,BALICA N,et al.Mucosal melanomas in the elderly:challenging cases and review of the literature[J].Clin Interv Aging,2014,9:929-937.
[2]	KUFUOR-MENSAH E,WATSON G L.Malignant melanomas in a penguin (Eudyptes chrysolophus) and a red-tailed hawk (Buteo jamaicensis)[J].Vet Pathol,1992,29(4):354-356.
[3]	ALGHAMDI S A,ZOROQUIAIN P,DIAS A B T,et al.Diagnostic value of SOX-10 immunohistochemical staining for the detection of uveal melanoma[J].Ecancermedicalscience,2015,9:566.
[4]	XIA J X,WANG Y L,LI F Q,et al.Expression of microphthalmia transcription factor,S100 protein,and HMB-45 in malignant melanoma and pigmented nevi[J].Biomed Rep,2016,5(3):327-331.
[5]	REID H A C,HERRON A J,HINES II M E,et al.Metastatic malignant melanoma in a mandarin duck (Aix galericulata)[J].Avian Dis,1993,37(4):1158-1162.
[6]	BARLOW A M,GIRLING T R.Malignant melanoma in a merlin (Falco columbarius)[J].Vet Rec,2004,154(22):696-697.
[7]	KAJIGAYA H,KONAGAYA K,EJIMA H,et al.Metastatic melanoma appearing to originate from the beak of a racing pigeon (Columba livia)[J].Avian Dis,2010,54(2):958-960.
[8]	FELTRER RAMBAUD Y,FLACH E J,FREEMAN K P.Malignant melanoma in a Humboldt penguin (Spheniscus humboldti)[J].Vet Rec,2003,153(7):217-218.
[9]	WILLIAMS S M,ZAVALA G,HAFNER S,et al.Metastatic melanomas in young broiler chickens (Gallus gallus domesticus)[J].Vet Pathol,2012,49(2):288-291.
[10]	WILLIS B C,JOHNSON G,WANG J,et al.SOX10:a useful marker for identifying metastatic melanoma in sentinel lymph nodes[J].Appl Immunohistochem Mol Morphol,2015,23(2):109-112.
[11]	PALLA B,SU A,BINDER S,et al.SOX10 expression distinguishes desmoplastic melanoma from its histologic mimics[J].Am J Dermatopathol,2013,35(5):576-581.
[12]	RAMOS-HERBERTH F I,KARAMCHANDANI J,KIM J,et al.SOX10 immunostaining distinguishes desmoplastic melanoma from excision scar[J].J Cutaneous Pathol,2010,37(9):944-952.
[13]	NIELSEN P S,RIBER-HANSEN R,STEINICHE T.Immunohistochemical double stains against Ki67/MART1 and HMB45/MITF:promising diagnostic tools in melanocytic lesions[J].Am J Dermatopathol,2011,33(4):361-370.
[14]	DUNCAN A E,SMEDLEY R,ANTHONY S,et al.Malignant melanoma in the penguin:characterization of the clinical,histologic,and immunohistochemical features of malignant melanoma in 10 individuals from three species of penguin[J].J Zoo Wildl Med,2014,45(3):534-549.

[1]	刘佳惠, 吴开开, 王磊, 张康, 韩松伟, 陈富斌, 徐国伟, 郭志廷, 古雪艳, 张景艳, 李建喜. 黄芪多糖、皂苷及益生菌复合物对感染大肠杆菌肉鸡肠道的保护作用[J]. 畜牧兽医学报, 2024, 55(5): 2241-2252.
[2]	罗通旺, 吴亚, 王书杰, 宋厚辉, 邵春艳. 镉致肝损伤机制及硒拮抗镉肝毒性的研究进展[J]. 畜牧兽医学报, 2024, 55(4): 1456-1466.
[3]	梁灿新, 郑小雪, 舒雪利, 周婉怡, 廖明, 曹伟胜. 与鸡内皮血管瘤病例相关的禽白血病病毒K亚群分离及其gp85基因演化分析[J]. 畜牧兽医学报, 2024, 55(3): 1127-1136.
[4]	张志飞, 唐雪颖, 闵力, 童雄, 陈卫东, 巨向红, 李大刚. 荷斯坦奶牛肝脏组织中与泌乳时期及繁殖力相关的基因共表达网络构建[J]. 畜牧兽医学报, 2024, 55(2): 528-539.
[5]	宋明强, 解竞静, 欧娟, 王钰明, 侯嘉, 谭高明, 田凯, 朱云, 萨仁娜, 赵峰. 盐酸不溶灰分测定方法影响肉鸡饲粮代谢能准确性的比较研究[J]. 畜牧兽医学报, 2024, 55(2): 619-628.
[6]	王栋, 柳可欣, 何炎峻, 邓守翔, 刘云, 马卫明. 饲粮中添加腐殖酸钠对鼠伤寒沙门菌感染肉鸡肝组织炎症和抗氧化能力的影响[J]. 畜牧兽医学报, 2024, 55(2): 629-639.
[7]	邱文粤, 苏依曼, 叶嘉莉, 章心婷, 庞晓玥, 王荣梅, 谢子茂, 张辉, 唐兆新, 苏荣胜. 积雪草酸通过调控细胞凋亡和自噬缓解脂多糖诱导肉鸡急性肾损伤的研究[J]. 畜牧兽医学报, 2024, 55(2): 809-821.
[8]	唐鑫鑫, 郑炬梅, 骆娜, 营凡, 朱丹, 李森, 刘大伟, 安炳星, 文杰, 赵桂苹, 李和刚. 基于全基因组关联分析揭示肉鸡腿病发生的遗传机制[J]. 畜牧兽医学报, 2024, 55(1): 99-109.
[9]	张德安, 杨若渚, 刘杰, 刘德武, 邓铭, 柳广斌, 孙宝丽, 郭勇庆, 李耀坤. 饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析[J]. 畜牧兽医学报, 2024, 55(1): 232-244.
[10]	马亚军, 焦智慧, 刘笑凝, 陆翔羽, 刘涛, 王月, 朴晨曦, 王洪斌. 脂肪间充质干细胞对小型猪肝缺血再灌注合并肝切除组织细胞焦亡的影响[J]. 畜牧兽医学报, 2024, 55(1): 355-364.
[11]	张亚峰, 朱斌, 马畅, 张源淑. 二脒那秦激活ACE2对非酒精性脂肪肝病大鼠肝线粒体影响研究[J]. 畜牧兽医学报, 2023, 54(9): 3895-3904.
[12]	袁何玲, 方词, 黄金虎, 王晓明, 肖文浚, 刘睿婷, 史荣梅, 王丽平. 大蒜辣素对肉鸡肝组织中CYP1A2 mRNA表达及其酶动力学的影响[J]. 畜牧兽医学报, 2023, 54(9): 3905-3915.
[13]	赵阳飞, 于洋欢, 王金明, 张建海, 孙子龙, 牛瑞燕, 王俊东. IL-17A基因敲除对氟诱导小鼠肝炎症反应和肝细胞凋亡的影响[J]. 畜牧兽医学报, 2023, 54(7): 3108-3117.
[14]	王政, 郭文婕, 程瑾, 原一桐, 罗榕, 薛毅, 张利环, 朱芷葳, 李慧锋. 营养转运相关基因调控区多态性与黄羽肉鸡饲料转化率关联分析[J]. 畜牧兽医学报, 2023, 54(6): 2343-2352.
[15]	赵栋皓, 原梦, 马凯腾, 段卓, 祝一鑫, 唐芳, 韩克光, 霍乃蕊. 羊骨胶原肽对镉的螯合作用及对镉致鸡肝损伤的干预作用[J]. 畜牧兽医学报, 2023, 54(6): 2641-2652.

1例肉鸡肝恶性黑色素瘤的病理学诊断

Pathological Diagnosis of a Case of Liver Malignant Melanoma in Broiler Chicken

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 14

相关文章 15

编辑推荐

Metrics

本文评价