影响马胚胎移植成功率的关键因素分析

doi:10.11843/j.issn.0366-6964.2020.01.010

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (1): 83-89.doi: 10.11843/j.issn.0366-6964.2020.01.010

影响马胚胎移植成功率的关键因素分析

李楠¹, 王涛², 张凤龙³, 张翔¹, 李平岁¹, 韩国才^1*, 曾申明^1*

1. 中国农业大学动物科技学院, 北京 100193;
2. 国家胶类中药工程技术研究中心, 东阿阿胶股份有限公司, 东阿 252201;
3. 武汉商学院, 武汉 430050

收稿日期:2019-08-19 出版日期:2020-01-23 发布日期:2020-01-17
通讯作者: 曾申明,主要从事马属动物繁殖技术研究,E-mail:zengsm@cau.edu.cn;韩国才,主要从事马属动物遗传育种与繁殖研究,E-mail:hanguocai@263.net
作者简介:李楠(1990-),男,黑龙江齐齐哈尔人,博士,主要从事马属动胚胎移植、胚胎冷冻及精液冷冻研究,E-mail:linan_cau@163.com;王涛(1984-),男,山东潍坊人,主要从事马属动物繁殖和临床疾病研究,E-mail:506582703@qq.com。
基金资助:
国家公益性行业（农业）科研专项“马驴产业技术研究与试验示范”（201003075）

The Analysis of Key Factors Affecting the Success Rate of Equine Embryo Transfer

LI Nan¹, WANG Tao², ZHANG Fenglong³, ZHANG Xiang¹, LI Pingsui¹, HAN Guocai^1*, ZENG Shenming^1*

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e 252201, China;
3. Wuhan Business University, Wuhan 430050, China

Received:2019-08-19 Online:2020-01-23 Published:2020-01-17

摘要/Abstract

摘要： 旨在探讨影响马胚胎移植效率的几种关键因素。本研究统计了国内北京马场、河北马场和山东马场2013-2018年胚胎移植数据，3个马场供体马数量分别为15、21和25匹，受体母马数量分别为56、50和75匹。所有母马年龄为3~12岁。统计供体马冲胚时间对胚胎回收率的影响；胚胎日龄对移植后受体马妊娠率的影响；供、受体母马排卵同期化程度对移植后妊娠率的影响；受体母马居住移植基地时间对移植后妊娠率的影响。结果显示，母马在配种季节注射前列腺素（PG）+GnRH类似物或PG+hCG诱发排卵，发情周期分别为（14.5±0.8）和（14.3±1.1）d，显著低于对照组的（（20.5±2.6）d，P<0.05）；排卵后第8天冲洗子宫的胚胎回收率均高于第7天，但差异不显著；8日龄胚胎移植后受体马的妊娠率均高于7日龄，差异不显著；供体母马排卵比受体母马早1 d时，胚胎移植后的妊娠率最高；受体母马在移植基地居住时间大于1年时，移植后妊娠率高于居住时间小于0.5年的受体马。根据以上结果，本研究得出如下结论，PG与hCG或GnRH类似物联合使用可缩短母马发情周期，母马排卵后第8天的胚胎回收率和移植后妊娠率较高，胚胎移植时选择居住时间大于1年且排卵时间比供体晚1 d的母马作受体。

关键词: 马, 胚胎移植, 妊娠率

Abstract: This study aimed to research factors affecting the efficiency of equine embryo transfer. The data of embryo transfer in 3 horse farms from 2013 to 2018 were analyzed.The number of donor horses in the 3 farms were 15, 21 and 25, respectively, and the number of recipient mares were 56, 50 and 75 respectively. All mares were 3 to 12 years old. The following items were counted:The effect of flushing day post ovulation of donor horses on the embryo recovery rate; The effect of embryonic age on the pregnancy rate of recipient mares after transplantation; The effect of the degree of ovulation synchronization of the donor and recipient mares on the pregnancy rate after transplantation; The effect of residence time at farm of recipient mares on pregnancy rate after transplantation. The results indicated that, mares were injected with prostaglandin (PG)+GnRH analogues or PG + hCG to induce ovulation during the breeding season, the estrous cycle were (14.5±0.8) and (14.3±1.1)d, respectively, which was significantly lower than the control group ((20.5±2.6) d, P<0.05). The embryo recovery rate of washing the uterus on the 8th day after ovulation was higher than that on the 7th day, but the difference was not significant; The pregnancy rate of recipient horses after 8-day-old embryo transfer was higher than that of 7-day-old, and the difference was not significant; After embryo tranfer, the recipients which ovulated 1 day later than donors had the highest pregnancy rate. When the residence time at farm of recipients was longer than 1 year, the pregnancy rate was significantly higher than those shorter than 0.5 year. In conclusion, the injection of PG + hCG or PG + GnRH anologue could shorten estrus cycle of mares. The embryo recovery rate and the pregnancy rate after transplantation of the 8th day embryo after ovulation were higher. When the embryos were transplanted, the mares with the residence time at farm more than 1 year and the ovulation time 1 day later than the donors were used as recipients.

Key words: horse, embryo transfer, pregnancy rate

中图分类号:

S821.3

李楠, 王涛, 张凤龙, 张翔, 李平岁, 韩国才, 曾申明. 影响马胚胎移植成功率的关键因素分析[J]. 畜牧兽医学报, 2020, 51(1): 83-89.

LI Nan, WANG Tao, ZHANG Fenglong, ZHANG Xiang, LI Pingsui, HAN Guocai, ZENG Shenming. The Analysis of Key Factors Affecting the Success Rate of Equine Embryo Transfer[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 83-89.

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影响马胚胎移植成功率的关键因素分析

The Analysis of Key Factors Affecting the Success Rate of Equine Embryo Transfer

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