gga-miR-155对MDCC-MSB1细胞转录组的影响

doi:10.11843/j.issn.0366-6964.2023.02.023

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (2): 663-672.doi: 10.11843/j.issn.0366-6964.2023.02.023

gga-miR-155对MDCC-MSB1细胞转录组的影响

余祖华^1,2,3, 贾艳艳^1,2,3, 何雷^1,2,3, 廖成水^1,2,3, 李静^1,2,3, 魏颖^1,2,3, 陈建^1,2,3, 陈松彪^1,2,3, 尚珂^1,2,3, 丁轲^1,2,3*

1. 洛阳活载体生物材料与动物疫病重点实验室, 洛阳 471003;
2. 河南科技大学功能微生物与畜禽健康实验室, 洛阳 471003;
3. 河南科技大学动物科技学院, 洛阳 471003

收稿日期:2022-07-01 出版日期:2023-02-23 发布日期:2023-02-21
通讯作者: 丁轲,主要从事动物疫病病原及动物微生态研究,E-mail:keding19@163.com
作者简介:余祖华(1977-)女,河南商城人,副教授,博士,主要从事分子免疫学及动物微生态研究,E-mail:yzhd05@163.com
基金资助:
国家自然科学基金（U1504308；31702207）；河南科技大学省部级科技创新平台培育项目（2015SPT004）

Effects of gga-miR-155 on MDCC-MSB1 Cell Transcriptomes

YU Zuhua^1,2,3, JIA Yanyan^1,2,3, HE Lei^1,2,3, LIAO Chengshui^1,2,3, LI Jing^1,2,3, WEI Ying^1,2,3, CHEN Jian^1,2,3, CHEN Songbiao^1,2,3, SHANG Ke^1,2,3, DING Ke^1,2,3*

1. Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang 471003, China;
2. Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology, Luoyang 471003, China;
3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received:2022-07-01 Online:2023-02-23 Published:2023-02-21

摘要/Abstract

摘要： 旨在探讨gga-miR-155对马立克病病毒转化的肿瘤细胞系MDCC-MSB1细胞转录组水平变化的影响。本研究以MDCC-MSB1细胞为研究对象，首先将gga-miR-155模拟物及其阴性对照转染MDCC-MSB1细胞，在转染后48 h提取总RNA，用安捷伦2100核酸检测仪分析各组细胞总RNA的完整性，采用RT-qPCR分析gga-miR-155在MDCC-MSB1细胞中的过表达情况；然后利用高通量测序技术，分析gga-miR-155过表达后MDCC-MSB1细胞转录水平的变化，筛选差异表达基因，结合生物信息学分析预测差异表达基因中gga-miR-155靶mRNA。结果显示：制备了gga-miR-155在MDCC-MSB1细胞过表达样本并构建了高通量测序文库；与对照组相比，gga-miR-155过表达组的差异表达基因有87个，其中，上调表达的基因有47个，下调表达的基因有40个；GO与KEGG分析显示，这些差异表达基因显著富集于代谢和蛋白结合过程/信号通路；通过Targetscan和miRada软件预测下调表达基因中鸡BACH1为gga-miR-155的靶mRNA （P<0.05）。综上，过表达gga-miR-155可调控MDCC-MSB1细胞的转录组水平，差异表达基因中BACH1基因可能在gga-miR-155的作用下参与了MDCC-MSB1细胞的代谢过程。

关键词: gga-miR-155, 马立克病, MDCC-MSB1细胞, 转录组

Abstract: This study aimed to investigate the effects of gga-miR-155 on the transcriptomic level of Marek's disease virus transformed tumor cell line MDCC-MSB1 cells. In this study, MDCC-MSB1 cells were transfected with gga-miR-155 mimics and gga-miR-155 mimics NC, the total RNA of MDCC-MSB1 cells transfected with gga-miR-155 mimics and its negative control was extracted at 48 h after transfection and the integrity of total RNA was analyzed by Agilent 2100 nucleic acid detector respectively. After analyzing the overexpression of gga-miR-155 in MDCC-MSB1 cells by RT-qPCR, the changes in transcription level of MDCC-MSB1 cells after overexpression of gga-miR-155 were analyzed using the high-throughput sequencing technology. On this basis, the differentially expressed genes were screened, and the involved target mRNAs of gga-miR-155 in the differentially expressed genes were predicted combined with bioinformatics analysis. The RNA samples of gga-miR-155 overexpression in MDCC-MSB1 cells were prepared and a high-throughput sequencing library was constructed. Compared with the negative control group, 87 differentially expressed genes were found in the gga-miR-155 overexpression group, including 47 up-regulated genes and 40 down-regulated genes. GO and KEGG analysis showed that these differentially expressed genes were significantly enriched in metabolic and protein binding processes/signaling pathways. Chicken BACH1 was predicted as the target mRNA of gga-miR-155 by Targetscan and miRada software (P<0.05). The transcriptional level of MDCC-MSB1 cells was regulated by overexpressed gga-miR-155, and BACH1 gene in the differentially expressed genes may be involved in the metabolic process of MDCC-MSB1 cells under the regulation of gga-miR-155.

Key words: gga-miR-155, Marek's disease, MDCC-MSB1 cells, transcriptome

中图分类号:

S831.7

余祖华, 贾艳艳, 何雷, 廖成水, 李静, 魏颖, 陈建, 陈松彪, 尚珂, 丁轲. gga-miR-155对MDCC-MSB1细胞转录组的影响[J]. 畜牧兽医学报, 2023, 54(2): 663-672.

YU Zuhua, JIA Yanyan, HE Lei, LIAO Chengshui, LI Jing, WEI Ying, CHEN Jian, CHEN Songbiao, SHANG Ke, DING Ke. Effects of gga-miR-155 on MDCC-MSB1 Cell Transcriptomes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 663-672.

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gga-miR-155对MDCC-MSB1细胞转录组的影响

Effects of gga-miR-155 on MDCC-MSB1 Cell Transcriptomes

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