图们江流域部分市县蜱传病原体分子流行病学及蜱体内菌群调查

doi:10.11843/j.issn.0366-6964.2025.06.035

摘要/Abstract

摘要：

为系统了解图们江流域蜱携带病原情况及蜱体内菌群，本试验对图们江流域血蜱属与革蜱属的蜱传病原体分子流行病学、蜱体内菌群进行调查。在图们江流域3个市县的5个采样点采集到森林革蜱、日本血蜱和嗜群血蜱。采用高通量测序技术对蜱携带细菌进行调查，对147只蜱携带细菌16S基因的V3-V4可变区进行二代测序分析；对442只蜱虫携带斑点热群立克次体、无形体和巴贝斯虫进行PCR检测。研究结果显示，高通量测序结果分析发现存在至少2种蜱传病原体，6种属内存在部分致病性菌种的潜在致病菌属。3个市县不同种属、不同性别之间的蜱携带的微生物菌群存在差异。在该地区蜱中检测出1种斑点热群立克次体(饶氏立克次体)，1种无形体(山羊无形体)，1种巴贝斯虫(驽巴贝斯虫)。所获结果为该地区的蜱携带病原体、微生物菌群提供了基础数据，为蜱类和蜱传疾病的防控提供数据支持。

关键词: 图们江流域, 蜱, 蜱传病原体, 蜱体内菌群, 调查

Abstract:

In order to systematically understand the tick-borne pathogens in the Tumen River Basin and to investigate the tick flora, the molecular epidemiology of tick-borne pathogens of Haemaphysalis and Dermacentor as well as the tick flora in the Tumen River Basin were investigated in the experiment. Dermacentor silvarum, Haemaphysalis japonica, Haemaphysalis concinna were collected at 5 sampling points in 3 cities and counties in the Tumen River basin. Tick-borne bacteria were investigated using high-throughput sequencing technology, and 147 ticks were analysed by second-generation sequencing of the V3-V4 variable region of the 16S gene of tick-borne bacteria. PCR was performed on 442 ticks carrying spotted fever groups Rickettsia, Anaplasma and Babesia. The results of the study showed that analyses of high-throughput sequencing results revealed the presence of at least two tick-borne pathogens and six potentially pathogenic genera with some pathogenic strains within the genus. There were differences in microflora carried by ticks of different genera and sexes in three cities and counties. One type of Rickettsia was detected in ticks in the region: Rickettsia raoulttii; One type of Anaplasma: Anaplasma capra; One species of Babesia: Babesia caballi. The obtained results provide basic data for tick borne pathogens and microbial communities in the region, and provide data support for the prevention and control of ticks and tick borne diseases.

Key words: Tumen river basin, ticks, tick borne pathogens, tick microbiota, investigation

中图分类号:

S852.746

殷国璐, 于天, 王金琦, 徐颖, 刘喆, 高旭, 于龙政, 汲生威, 薛书江, 金东春. 图们江流域部分市县蜱传病原体分子流行病学及蜱体内菌群调查[J]. 畜牧兽医学报, 2025, 56(6): 2927-2936.

YIN Guolu, YU Tian, WANG Jinqi, XU Ying, LIU Zhe, GAO Xu, YU Longzheng, JI Shengwei, XUE Shujiang, JIN Dongchun. Molecular Epidemiology of Tick Borne Pathogens and Investigation of Tick Borne Microbiota in Some Cities and Counties in the Tumen River Basin[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2927-2936.

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