羊蜱蝇携带巴尔通体和斑点热群立克次体PCR检测及遗传关系分析

doi:10.11843/j.issn.0366-6964.2021.06.024

摘要/Abstract

摘要： 为了解四川省石渠县羊蜱蝇巴尔通体和斑点热群立克次体感染情况，采集藏绵羊体表羊蜱蝇，经形态学鉴定后，提取羊蜱蝇总DNA，PCR扩增巴尔通体gltA和rpoB基因、斑点热群立克次体OmpA和OmpB基因，对阳性产物测序、比对及构建进化树，从而确定羊蜱蝇感染巴尔通体和斑点热群立克次体的种类及感染率。在石渠县的4个乡镇总计采集到407只羊蜱蝇成虫，4个乡镇均检出了Bartonella melophagi，总感染率为14.0%（57/407）。在阿日扎镇、呷衣乡和长沙贡马乡检出了Rickettsia raoultii，总感染率为11.1%（45/407），且长沙贡马乡感染率显著高于阿日扎镇和呷衣乡（P<0.05）；在新荣乡检出了Rickettsia sp.，感染率为6.6%（8/121）。本次试验中，未发现混合感染。石渠县藏绵羊源羊蜱蝇携带巴尔通体和立克次体，首次在石渠县藏绵羊源羊蜱蝇中检出了B.melophagi、R.raoultii和Rickettsia sp.。

关键词: 巴尔通体, 斑点热群立克次体, 羊蜱蝇, 石渠县

Abstract: This experiment was conducted to investigate infection of Bartonella and spotted fever group rickettsiae in Melophagus ovinus in Shiqu County, Sichuan Province. M. ovinus collected from Tibetan sheep were classified by morphological identification. Total DNA of all M. ovinus were extracted and partial sequences of gltA and rpoB genes of Bartonella, and OmpA and OmpB genes of Rickettsia were amplified by PCR, respectively. The positive products were sequenced and compared through the NCBI database and phylogenetic trees were constructed based on gltA, rpoB, OmpA and OmpB for determination of Bartonella and Rickettsia, respectively. A total of 407 adult M. ovinus were collected in four villages in Shiqu County. Bartonella melophagi was detected in all four villages, with a total infection rate of 14.0% (57/407). Rickettsia raoultii was detected in Arizha, Gayi and Changxgma, with a total infection rate of 11.1% (45/407). The infection rate in Changxgma was significantly higher than that in Arizha and Gayi (P<0.05). Rickettsia sp. was only detected in Xinrong, with a total infection rate of 6.6% (8/121). No co-infection was observed in this study. In Shiqu, Tibetan sheep is the biological vector of Bartonella and Rickettsia. In this study, B. melophagi, R. raoultii and Rickettsia sp. were first detected in M. ovinus in Shiqu County.

Key words: Bartonella, SFG rickettsiae, Melophagus ovinus, Shiqu County

中图分类号:

向阳, 袁东波, 侯巍, 莫茜, 尹杰, 阳爱国, 郝力力. 羊蜱蝇携带巴尔通体和斑点热群立克次体PCR检测及遗传关系分析[J]. 畜牧兽医学报, 2021, 52(6): 1700-1708.

XIANG Yang, YUAN Dongbo, HOU Wei, MO Xi, YIN Jie, YANG Aiguo, HAO Lili. PCR Detection and Genetic Relationship Analysis of Bartonella and Spotted Fever Group Rickettsiae in Melophagus ovinus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1700-1708.

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