畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (3): 1333-1340.doi: 10.11843/j.issn.0366-6964.2023.03.043

• 研究简报 • 上一篇    下一篇

牛腺病毒3型感染状况及其fiber轴区基因检测

何姝凡1, 邹沅彤1, 黄智兰1, 李倩1, 降措翁西1, 岳华1,2, 汤承1,2, 刘杰1,2*   

  1. 1. 西南民族大学畜牧兽医学院, 成都 610041;
    2. 青藏高原动物遗传资源保护与利用教育部重点实验室, 成都 610041
  • 收稿日期:2022-07-25 出版日期:2023-03-23 发布日期:2023-03-21
  • 通讯作者: 刘杰,主要从事动物呼吸道病毒病研究,E-mail:jieliuhzau@163.com
  • 作者简介:何姝凡(1996-),女,四川南充人,硕士生,主要从事动物呼吸道疾病诊断技术研究,E-mail:1196603683@qq.com
  • 基金资助:
    西南民族大学中央高校基本科研业务费专项资金项目(ZYN2023041);四川省自然科学基金(2023NSFSC1217);四川省肉牛创新团队项目(SCCXTD202013);“十四五”国家重点研发计划课题(2021YFD1600203)

Detection of Bovine Adenovirus Type 3 Infection Status and Its Fiber Shaft Gene

HE Shufan1, ZOU Yuantong1, HUANG Zhilan1, LI Qian1, JIANGCUO Wengxi1, YUE Hua1,2, TANG Cheng1,2, LIU Jie1,2*   

  1. 1. College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;
    2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, China
  • Received:2022-07-25 Online:2023-03-23 Published:2023-03-21

摘要: 本研究旨在通过TB Green Real-time PCR的方法对国内6个省(四川省、山西省、河南省、河北省、江苏省和内蒙古自治区)的9个规模化养牛场140份以流鼻涕、发热、咳嗽、呼吸困难为特征的病牛呼吸道鼻拭子样本开展牛腺病毒3型(bovine adenovirus type 3, BAdV-3)感染状况调查,并对其中的阳性样本进行fiber轴区基因检测。结果显示,140份样品中BAdV-3阳性率为36.4%,除河北省以外,其余各省均有BAdV-3检出,其中,四川及河南省检出率最高,分别达到82.9%(29/35)和83.3%(10/12)。其中,传统型占总阳性比的80.4%,fiber 轴区缺失毒株占总阳性样本的19.6%。缺失位置与数量一致,轴区连续缺失237个碱基对 (79个氨基酸),且缺失型仅在四川和河南两省检出,检出率分别为24.1%(7/29)和30%(3/10),场群阳性率分别为100%(2/2)和100%(1/1)。结果表明,BAdV-3已在我国牛场中广泛分布,目前流行的主要以传统型毒株为主,fiber轴区缺失毒株在我国具有独特的地域分布,主要在四川和河南省流行。

关键词: 牛腺病毒3型, 流行病学调查, 荧光定量PCR, 遗传进化, fiber轴区基因缺失

Abstract: This study was conducted to investigate the infection status of BAdV-3 by TB Green Real-time PCR on 140 nasal swab samples of sick cattle characterized by runny nose, fever, cough and dyspnea from 9 large-scale cattle farms in 6 provinces in China (Sichuan Province, Shanxi Province, Henan Province, Hebei Province, Jiangsu Province and Inner Mongolia Autonomous Region), and the positive samples were tested for the fiber shaft gene. The results showed that the BAdV-3 positive rate was 36.4% among 140 samples, and BAdV-3 was detected in all provinces except for Hebei Province, with the highest detection rates in Sichuan and Henan Provinces, reaching 82.9% (29/35) and 83.3% (10/12), respectively. Among them, the traditional types accounted for 80.4% of the total positive ratio, and the strain with the deletion of the fiber shaft region accounted for 19.6% of the total positive samples. The position and number of deletions were consistent, with 237 consecutive base pairs missing in the shaft region, and the deletion type was only detected in Sichuan and Henan Provinces, with a detection rate of 24.1% (7/29) and 30% (3/10), and a field group positive rate of 100% (2/2) and 100% (1/1), respectively. The results indicate that BAdV-3 has a wide distribution in cattle farms in China, and the current epidemic virus is mainly the traditional strain of BAdV-3. The fiber shaft gene deletion strain has a unique geographical distribution in China and is mainly prevalent in Sichuan and Henan Provinces.

Key words: bovine adenovirus type 3, epidemiological investigation, real-time PCR, genetics and evolution, the gene deletion in the fiber shaft region

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