猪瘟病毒中和抗体与E2蛋白抗体相关性分析

doi:10.11843/j.issn.0366-6964.2024.10.030

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (10): 4590-4596.doi: 10.11843/j.issn.0366-6964.2024.10.030

猪瘟病毒中和抗体与E2蛋白抗体相关性分析

夏应菊¹(), 李琰¹(), 刘俊², 徐嫄¹, 李芳韬¹, 邹兴启¹, 李琪¹, 李佳昕¹, 赵俊杰¹, 张乾义¹, 刘业兵¹^,*(), 徐璐¹^,*()

1. 中国兽医药品监察所, 国家猪瘟参考实验室, 北京 100081
2. 辽宁省动物疫病预防控制中心, 沈阳 110015

收稿日期:2023-12-04 出版日期:2024-10-23 发布日期:2024-11-04
通讯作者: 刘业兵,徐璐 E-mail:vet_xiayj@163.com;liyannongda@foxmail.com;zjsliuyebing@163.com;xulu777_ivdc@163.com
作者简介:夏应菊(1982-), 女, 四川成都人, 博士, 主要从事猪瘟及非洲猪瘟病毒检测及免疫机制研究, E-mail: vet_xiayj@163.com
李琰(1989-), 女, 黑龙江哈尔滨人, 硕士, 主要从事动物疫苗检验及检测方法研究, E-mail: liyannongda@foxmail.com
第一联系人：
夏应菊和李琰为同等贡献作者
基金资助:
国家重点研发计划(2022YFD1800601);国家重点研发计划(2022YFD1800605)

Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus

Yingju XIA¹(), Yan LI¹(), Jun LIU², Yuan XU¹, Fangtao LI¹, Xingqi ZOU¹, Qi LI¹, Jiaxin LI¹, Junjie ZHAO¹, Qianyi ZHANG¹, Yebing LIU¹^,*(), Lu XU¹^,*()

1. National Reference Laboratory for Classical Swine Fever, China Institute of Veterinary Drug Control, Beijing 100081, China
2. Liaoning Animal Disease Prevention and Control Center, Shenyang 110015, China

Received:2023-12-04 Online:2024-10-23 Published:2024-11-04
Contact: Yebing LIU, Lu XU E-mail:vet_xiayj@163.com;liyannongda@foxmail.com;zjsliuyebing@163.com;xulu777_ivdc@163.com

摘要/Abstract

摘要：

旨在探索猪瘟病毒中和抗体与E2蛋白抗体的关系，本研究对289份猪血清进行中和抗体效价和E2抗体测定，比较分析二者的相关性；同时从中选取15份血清，测定血清对不同猪瘟病毒流行野毒株的中和效价。结果表明，从整体上说E2抗体水平与中和效价呈正相关。当群体中所有个体E2抗体阻断率均大于60%时，才能保证群体70%以上免疫猪的中和抗体达到保护标准。本研究的结果有助于基层兽医工作者更好地评估临床中猪瘟疫苗的免疫保护状况，降低猪瘟疫情发生风险。

关键词: 猪瘟病毒, 中和抗体, E2抗体, 相关性

Abstract:

In order to explore the correlation between neutralizing antibody and E2 antibody against classical swine fever virus (CSFV), 289 swine sera were detected using both neutralization assay and ELISA among which neutralizing antibody titers of 15 sera were further tested by neutralization assay using different CSFV strains. The results showed that E2 antibody level against CSFV was positive correlated with neutralizing antibody titer. When the individual percentage inhibition of E2 antibody was over 60%, the neutralizing antibody titer of more than 70% vaccinated pigs in the herd meet the protection standard. These results could help the local veterinaries to evaluate the efficacy of CSFV vaccine better in the field and reduce the risk of CSF outbreak.

Key words: classical swine fever virus, neutralizing antibody, E2 antibody, correlation

中图分类号:

夏应菊, 李琰, 刘俊, 徐嫄, 李芳韬, 邹兴启, 李琪, 李佳昕, 赵俊杰, 张乾义, 刘业兵, 徐璐. 猪瘟病毒中和抗体与E2蛋白抗体相关性分析[J]. 畜牧兽医学报, 2024, 55(10): 4590-4596.

Yingju XIA, Yan LI, Jun LIU, Yuan XU, Fangtao LI, Xingqi ZOU, Qi LI, Jiaxin LI, Junjie ZHAO, Qianyi ZHANG, Yebing LIU, Lu XU. Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4590-4596.

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参考文献 24

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		E2抗体E2 antibody
		阳性 Positive	阴性 Negative	可疑 Suspicious	合计 Total	比率 Rate
中和抗体 Neutralizing antibody	阳性	201	12	10	223	0.772
	Positive	(a)	(b)	(e)	(a+b)	[r1=(a+b)/n]
	阴性	12	47	7	66	0.228
	Negative	(c)	(d)	(f)	(c+d)	[r2=(c+d)/n]
	合计	213	59	17	289
	total	(a+c)	(b+d)		(n=a+b+c+d+e+f)
	比率	0.737	0.204
	Rate	[r3=(a+c)/n]	[r4=(b+d)/n]

		E2抗体(阻断率) E2 antibody (PI)	中和效价对数 lgND₅₀
E2抗体(阻断率)	相关系数Pearson correlation	1	0.759^**
E2 antibody (PI)	显著性Sig. (2-tailed)		0.000
	N	289	289
中和效价对数	相关系数Pearson correlation	0.759^**	1
lgND₅₀	显著性Sig. (2-tailed)	0.000
	N	289	289

阻断率范围 Range of PI	中和效价对数lgND₅₀				免疫保护比例/% Percentage of protection
阻断率范围 Range of PI	0~1	1~1.5	≥1.5	合计Total	免疫保护比例/% Percentage of protection
0%~40%	54	7	15	76	19.7
40%~50%	4	11	11	26	42.3
50%~60%	5	6	24	35	68.6
60%~70%	2	5	18	25	72.0
70%~80%	1	5	35	41	85.4
80%~90%	0	1	55	56	98.2
90%~100%	0	0	30	30	100.0

血清编号 No. of serum	中和效价(ND₅₀)Neutralization titer			E2抗体E2 antibody
血清编号 No. of serum	SM (1.1亚型)	HuB (2.1亚型)	HeB (2.2亚型)	阻断率/% PI	判定 Results
1	1 280	240	640	91.00	阳性Positive
2	960	160	240	90.09	阳性Positive
3	1280	240	480	89.88	阳性Positive
4	60	60	40	69.45	阳性Positive
5	10	10	20	59.36	阳性Positive
6	20	40	60	56.97	阳性Positive
7	20	15	20	41.11	阳性Positive
8	N	40	20	39.82	可疑Doubtful
9	N	10	20	35.86	可疑Doubtful
10	10	N	N	35.32	可疑Doubtful
11	10	20	30	34.59	可疑Doubtful
12	10	10	N	27.87	阴性Negative
13	N	N	N	14.19	阴性Negative
14	N	15	N	10.62	阴性Negative
15	N	N	N	9.64	阴性Negative

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猪瘟病毒中和抗体与E2蛋白抗体相关性分析

Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus

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