母猪子宫内膜炎阴道菌群与血清促炎细胞因子的变化及其相关性分析

doi:10.11843/j.issn.0366-6964.2024.08.040

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (8): 3688-3698.doi: 10.11843/j.issn.0366-6964.2024.08.040

母猪子宫内膜炎阴道菌群与血清促炎细胞因子的变化及其相关性分析

鲜婷婷¹^,²^,³(), 刘彦²^,³, 曹忻¹^,*(), 冯涛²^,³^,*()

1. 西北民族大学生命科学与工程学院, 兰州 730030
2. 北京市农林科学院畜牧兽医研究所, 北京 100097
3. 北京市农林科学院-美国俄克拉荷马州立大学动物科学联合实验室, 北京 100097

收稿日期:2023-11-21 出版日期:2024-08-23 发布日期:2024-08-28
通讯作者: 曹忻,冯涛 E-mail:xtt001021@163.com;caoxin-juliet@163.com;fengtao_gs@163.com
作者简介:鲜婷婷(1999-)，女，四川仪陇人，硕士生，主要从事动物繁殖调控研究，E-mail: xtt001021@163.com
基金资助:
国家自然科学基金项目(31972575);北京市农林科学院课题(CZZJ202205);中央高校专项基金项目(31920240078)

Analysis of the Changes of Vaginal Microflora and Serum Pro-inflammatory Cytokines and Their Correlation in Sows with Endometritis

Tingting XIAN¹^,²^,³(), Yan LIU²^,³, Xin CAO¹^,*(), Tao FENG²^,³^,*()

1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China
2. Institute of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing 100097, China
3. Joint Laboratory of Animal Science between IAHVM of BAAFS and Division of Agricultural Science and Natural Resource of Oklahoma State University, Beijing 100097, China

Received:2023-11-21 Online:2024-08-23 Published:2024-08-28
Contact: Xin CAO, Tao FENG E-mail:xtt001021@163.com;caoxin-juliet@163.com;fengtao_gs@163.com

摘要/Abstract

摘要：

子宫内膜炎是规模化猪场常发疾病之一，但其致病机制尚未完全明确。本研究旨在分析患病母猪阴道黏液菌群多样性及其与血清促炎细胞因子的相关性，揭示母猪子宫内膜炎主要致病菌种类。选取北京郊区某猪场分娩后健康(C组)和子宫内膜炎(E组)母猪各7头，采用酶联免疫吸附测定(ELISA)法测定血清促炎细胞因子白细胞介素(IL)-1α、IL-1β、IL-6、IL-8和肿瘤坏死因子α(TNF-α)水平，通过Illumina NovaSeq 6000测序平台对母猪阴道黏液菌群基因进行测序，利用联川生物云平台分析菌群多样性，并研究差异阴道菌群与促炎细胞因子的相关性。结果表明：E组IL-1α和IL-6的浓度水平显著高于C组(P < 0.01)；E组阴道微生物多样性显著下降(P < 0.05)；在门水平上，与C组相比，E组的厚壁菌门(Firmicutes)菌群相对丰度显著降低，变形菌门(Proteobacteria)菌群相对丰度显著升高(P < 0.01)；在属水平上，C组和E组中最主要的优势菌属分别是梭杆菌属(Fusobacterium)和埃希杆菌-志贺菌属(Escherichia-Shigella)；在E组中，IL-8与瘤胃球菌属(Ruminococcus)和气球菌属(Aerococcus)呈显著正相关关系(P < 0.05)；IL-6与埃希杆菌-志贺菌属呈显著正相关关系(P < 0.01)。子宫内膜炎母猪阴道黏液菌群中的有益菌减少，致病菌增加，血清促炎细胞因子水平较健康母猪升高，且血清促炎细胞因子水平与阴道黏液特定菌群数量密切相关，本研究为子宫内膜炎的防治提供了新的思路。

关键词: 母猪, 子宫内膜炎, 细胞因子, 阴道黏液菌群, 相关性

Abstract:

Endometritis is a prevalent disease in large-scale pig farms, but its pathogenic mechanism has not been fully clarified. This study aimed to analyze the diversity of vaginal microflora and its correlation with serum pro-inflammatory cytokines in sows with endometritis, with the ultimate goal of identifying the principal pathogenic bacteria responsible for endometritis in sows. Seven healthy (group C) and seven endometritis (group E) postpartum sows from a pig farm in the suburb of Beijing city were selected, respectively. Serum pro-inflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) levels were measured using enzyme-linked immunosorbent assay (ELISA). Genes in vaginal microflora of sows were sequenced through Illumina NovaSeq 6000 sequencing and microbial diversity were analyzed using the OmicStudio platform to evaluate the correlation between microbiota and pro-inflammatory factorscytokines. The results showed that concentrationsthe levels of IL-1α and IL-6 in group E were significantly higher than those in group C (P < 0.01). Vaginal microbial diversity was significantly lower in group E compared with group C (P < 0.05). At the phylum level, the relative abundance of Firmicutes in group E was drastically reduced compared with group C, while that of Proteobacteria was significantly increased in group E compared with group C (P < 0.01). At the genus level, the most dominant genera in group C and group E were Fusobacterium and Escherichia-Shigella, respectively. In group E, IL-8 was positively correlated with Ruminococcus and Aerococcus (P < 0.05), there was a significant positive correlation between IL-6 and Escherichia-Shigella (P < 0.01). In sows with endometritis, beneficial bacteria in the vaginal microflora were decreased, pathogenic bacteria were increased, serum pro-inflammatory cytokine levels were elevated compared with those in healthy sows. Serum pro-inflammatory cytokine levels were closely correlated with the number of certain vaginal microflora. This study can provide new insights for the prevention and therapy of endometritis in sows.

Key words: sows, endometritis, cytokines, vaginal mucus microflora, correlation

中图分类号:

S857.23

鲜婷婷, 刘彦, 曹忻, 冯涛. 母猪子宫内膜炎阴道菌群与血清促炎细胞因子的变化及其相关性分析[J]. 畜牧兽医学报, 2024, 55(8): 3688-3698.

Tingting XIAN, Yan LIU, Xin CAO, Tao FENG. Analysis of the Changes of Vaginal Microflora and Serum Pro-inflammatory Cytokines and Their Correlation in Sows with Endometritis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3688-3698.

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项目 Items	健康组 C group	子宫内膜炎组 E group
物种丰富度指数 Observed_species index	482.43±62.91^a	273.29±17.88^b
香农指数 Shannon index	5.45±0.35^a	3.61±0.45^b
辛普森指数 Simpson index	0.93±0.01^a	0.76±0.06^b
Chao1指数 Chao1 index	485.63±63.02^a	274.79±17.82^b
Pielou-e指数 Pielou-e index	0.61±0.03^a	0.45±0.05^b
菌群覆盖度指数 Goods_coverage index	1.00±0.00	1.00±0.00

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母猪子宫内膜炎阴道菌群与血清促炎细胞因子的变化及其相关性分析

Analysis of the Changes of Vaginal Microflora and Serum Pro-inflammatory Cytokines and Their Correlation in Sows with Endometritis

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