鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2022.12.038

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (12): 4527-4534.doi: 10.11843/j.issn.0366-6964.2022.12.038

鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱分析

尹磊^1,2, 潘孝成^1,2*, 沈学怀^1,2, 张丹俊^1,2, 戴银^1,2, 王洁茹^1,2

1. 安徽省农科院畜牧兽医研究所安徽省畜禽疫病研究中心, 合肥 230031;
2. 畜禽产品安全工程安徽省重点实验室, 合肥 230031

收稿日期:2021-11-05 出版日期:2022-12-23 发布日期:2022-12-25
通讯作者: 潘孝成，主要从事畜禽传染病防控研究，E-mail:pxcpyq@sina.com
作者简介:尹磊(1989-)，男，安徽合肥人，助理研究员，博士，主要从事畜禽病原菌致病机理研究，E-mail:yinlei1989@yeah.net
基金资助:
安徽省科技重大专项项目(202003b06020006；202003a06020012)；安徽省自然科学基金项目(2108085QC137)；安徽省家禽产业技术体系项目(AHCYJSTX-06)

Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks

YIN Lei^1,2, PAN Xiaocheng^1,2*, SHEN Xuehuai^1,2, ZHANG Danjun^1,2, DAI Yin^1,2, WANG Jieru^1,2

1. Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;
2. Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei 230031, China

Received:2021-11-05 Online:2022-12-23 Published:2022-12-25

摘要/Abstract

摘要： 旨在探究鸡白痢沙门菌(Salmonella enterica serovar Pullorum，S.Pullorum)感染雏鸡后骨髓miRNA的差异表达特征，为深入了解鸡白痢沙门菌的致病机制提供理论基础。将7日龄SPF雏鸡随机分为两组，分别口服鸡白痢沙门菌和PBS，于感染24 h后采集骨髓进行miRNA测序，筛选差异倍数≥2且P值≤0.05的差异表达miRNAs进行靶基因预测以及GO、KEGG富集分析，随机选取6个miRNAs进行qRT-PCR验证，构建与免疫过程相关的miRNA-mRNA靶点网络。通过miRNA测序，共获得20个已知的差异表达miRNAs，其中11个上调，9个下调。qRT-PCR结果表明，miRNA变化趋势与测序结果一致。GO分析结果表明，差异表达基因主要富集在膜运输、信号转导、免疫系统、碳水化合物代谢、糖类的生物合成和代谢等，KEGG的信号通路主要富集在Notch信号通路、Hedgehog信号通路、PPAR信号通路、AMPK信号通路、Hippo信号通路等，miRNA-mRNA网络互作发现，gga-miR-1466和gga-miR-6643-5p可能是参与免疫过程相关的关键miRNA。本研究解析了鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱特征，为了解鸡白痢沙门菌和鸡之间相互作用的复杂分子致病机制提供了新的见解，为防控鸡白痢沙门菌感染提供了新策略。

关键词: 鸡白痢沙门菌, 感染, 骨髓, miRNA, 免疫反应

Abstract: The aim of this study was to investigate the differential expression characteristics of miRNA in bone marrow of chicks infected with Salmonella enterica serovar Pullorum (S. Pullorum), thus to provide a theoretical basis for an in-depth understanding of the pathogenic mechanism of S. Pullorum. Seven-day-old SPF chicks were randomly divided into two groups and orally administered Salmonella enterica serovar Pullorum and PBS, respectively. Bone marrow was collected 24 h after infection for miRNA sequencing, and differentially expressed miRNAs with differential multiplicity ≥2 and P value ≤0.05 were screened for target gene prediction, GO and KEGG enrichment analysis, while six miRNAs of which were randomly selected for qRT-PCR validation. An miRNA-mRNA target network related to the immune process was constructed. A total of 20 known differentially expressed miRNAs were obtained by miRNA sequencing, of which 11 were up-regulated and 9 were down-regulated. qRT-PCR results showed that the miRNA change trend was consistent with the sequencing results. GO analysis showed that the differentially expressed genes were mainly enriched in membrane transport, signal transduction, immune system, carbohydrate metabolism, sugar biosynthesis and metabolism, etc. The signaling pathways of KEGG were mainly enriched in Notch signaling pathway, Hedgehog signaling pathway, PPAR signaling pathway, AMPK signaling pathway, Hippo signaling pathway, etc. The miRNA-mRNA network interactions identified gga-miR-1466 and gga-miR-6643-5p as possible key miRNAs involved in immune process. This experiment resolved the bone marrow miRNA expression profiles of S. Pullorum-infected chicks, providing new insights into the complex molecular pathogenesis of the interaction between S. Pullorum and chickens, and offering new strategies for the prevention and control of S. Pullorum infection in chickens.

Key words: Salmonella enterica serovar Pullorum, infection, bone marrow, miRNA, immune response

中图分类号:

S858.315.1

尹磊, 潘孝成, 沈学怀, 张丹俊, 戴银, 王洁茹. 鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱分析[J]. 畜牧兽医学报, 2022, 53(12): 4527-4534.

YIN Lei, PAN Xiaocheng, SHEN Xuehuai, ZHANG Danjun, DAI Yin, WANG Jieru. Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4527-4534.

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鸡白痢沙门菌感染雏鸡的骨髓miRNA表达谱分析

Analysis of Bone Marrow miRNA Expression Profiles in Salmonella enteric Serovar Pullorum-infected Chicks

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