不同生长时期梅花鹿鹿茸代谢组分析

doi:10.11843/j.issn.0366-6964.2022.12.037

摘要/Abstract

摘要： 旨在探究梅花鹿鹿茸不同生长时期小分子代谢物表达差异变化，为鹿茸快速生长与骨化分子机制的研究奠定基础。本研究选取体况良好的4岁龄雄性东北梅花鹿，收取生长25、45、65、100、130 d的鹿茸作为试验样品，每个时期3个生物学重复。利用UHPLC-TOF-MS技术对样品进行代谢组学分析。样品主成分分析与层次聚类分析结果显示，5个生长时期可分为鹿茸生长期(25、45与65 d)与鹿茸骨化期(100与130 d)两个阶段。筛选、鉴定到171种显著差异表达代谢物(VIP>1，P<0.05，|fold change|>2)，比对到HMDB数据库的112种代谢物，分为7大类；其中L-焦谷氨酸、L-组氨酸、L-肌肽与阿坎酸等有机酸类化合物在100 d含量最高，15-脱氧-Δ12,14-前列腺素J2、前列腺素H2、前列腺素I2在130 d显著上调，而其他氨基酸及有机酸类化合物均在生长期表达上调。差异代谢物显著富集到氨酰-tRNA生物合成、组氨酸代谢等13种KEGG代谢通路。本研究从代谢组水平为鹿茸快速生长与骨化分子机制的探究提供数据支撑。

关键词: 梅花鹿, 鹿茸, 不同生长时期, UHPLC-TOF-MS

Abstract: The purpose of this study was to explore the differential expression of small molecule metabolites in sika deer antler in different growth stages, and to lay a foundation for the study of the molecular mechanism of rapid growth and ossification of deer antler. Four years old male Northeast Sika deer in good condition were selected, and antler growing for 25, 45, 65, 100 and 130 d were collected as experimental samples, with 3 biological replicates in each period. Metabolomic analysis of the samples was performed by UHPLC-TOF-MS. The results of principal component analysis and hierarchical cluster analysis showed that the 5 growth periods could be divided into two stages: growth stage (25, 45 and 65 d) and ossification stage (100 and 130 d). A total of 171 significantly differentially expressed metabolites (VIP>1, P<0.05, |fold change|>2) were screened and identified, which were compared to 112 metabolites in the HMDB database, divided into 7 superclass. The contents of L-pyroglutamic acid, L-histidine, L-carnosine and acemic acid were the highest at 100 days, and 15-deoxy-δ12, 14-prostaglandin J2, prostaglandin H2 and prostaglandin I2 were significantly up-regulated at 130 days, while the expressions of other amino acids and organic acids were all up-regulated during the growth phase. The differential metabolites were significantly enriched in 13 KEGG metabolic pathways, such as aminoacyl-trNA biosynthesis and histidine metabolism. This study results provides data support for exploring the molecular mechanism of rapid growth and ossification of antler at the metabolome level.

Key words: sika deer, antler, different growth stages, UHPLC-TOF-MS

中图分类号:

S825.2

张然然, 荣敏, 董依萌, 王天骄, 邢秀梅. 不同生长时期梅花鹿鹿茸代谢组分析[J]. 畜牧兽医学报, 2022, 53(12): 4518-4526.

ZHANG Ranran, RONG Min, DONG Yimeng, WANG Tianjiao, XING Xiumei. Metabolomic Analysis of Sika Deer Antler in Different Growth Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4518-4526.

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