唾液乳杆菌对鸡毒支原体感染肉鸡生长性能及肺损伤的影响

doi:10.11843/j.issn.0366-6964.2022.09.031

摘要/Abstract

摘要： 本研究旨在探究唾液乳杆菌（Lactobacillus salivarius）对鸡毒支原体（Mycoplasma gallisepticum，MG）感染肉鸡生长性能及肺损伤的影响。选用1日龄AA肉鸡240只，按试验要求随机分为6组，包括对照组（Con）、低剂量唾液乳杆菌组（L）、高剂量唾液乳杆菌组（H）、MG感染组（MG）、MG感染+低剂量唾液乳杆菌组（MG+L）和MG感染+高剂量唾液乳杆菌组（MG+H）。Con组、MG组饲喂基础饲料，L组、MG+L组饲喂添加10⁸ CFU·kg^-1唾液乳杆菌的基础饲料，H组、MG+H组饲喂添加10⁹ CFU·kg^-1唾液乳杆菌的基础饲料，7日龄时给与MG组、MG+L组和MG+H组进行MG感染攻毒，饲养42 d后，通过分析各组鸡的体重、饲料转化率、肺部MG定植量、肺部病理损伤、肺部炎性损伤相关蛋白表达量及促炎性细胞因子含量来评价唾液乳杆菌缓解MG感染所致肉鸡生长性能下降及肺损伤效果。结果表明，MG感染极显著降低肉鸡体重（P<0.01），并极显著提高饲料转化率（P<0.01），而饲料中添加低剂量唾液乳杆菌和高剂量唾液乳杆菌均能极显著缓解MG感染所致肉鸡体重降低（P<0.01），并极显著改善饲料转化率（P<0.01）。饲料中添加低剂量唾液乳杆菌和高剂量唾液乳杆菌均能极显著降低MG肺部定植量（P<0.01）；明显改善MG感染所致肺部病理损伤；极显著降低MG感染所致炎性损伤相关蛋白（TLR2、HMGB1、p-p65/p65、NLRP3、Pro-Caspase-1/Caspase-1）表达（P<0.01）；极显著降低MG感染所致促炎性细胞因子（TNF-α、IL-1β、IL-6、IL-8）含量（P<0.01）。综上所述，饲料中添加唾液乳杆菌可以显著缓解MG感染所致肉鸡生长性能下降及肺损伤，该株唾液乳杆菌具有较大的应用潜力预防肉鸡感染MG。

关键词: 鸡毒支原体, 唾液乳杆菌, 生长性能, 肺损伤

Abstract: The purpose of the present study was to evaluate the effects of Lactobacillus salivarius on growth performance and lung injury of broiler chickens challenged with Mycoplasma gallisepticum (MG). Total of 240 one-day-old broiler chickens were randomly distributed into 6 groups, including control group (Con), low dose Lactobacillus salivarius group (L), high dose Lactobacillus salivarius group (H), MG infection group (MG), MG infection+low dose Lactobacillus salivarius group (MG+L), MG infection+high dose Lactobacillus salivarius group (MG+H). Broilers in Con group and MG group were fed control diet during experiment period; broilers in L group and MG+L group were fed control diet supplemented with Lactobacillus salivarius of 10⁸ CFU·kg^-1 feed during experiment period; broilers in H group and MG+H group were fed control diet supplemented with Lactobacillus salivarius of 10⁹ CFU·kg^-1 feed during experiment period; broilers in MG, MG+L and MG+H group were challenged with MG at 1 week of age. The effects of Lactobacillus salivarius supplementation on the body weight, feed conversion ratio, lung histopathology change, pulmonary MG colonization, pulmonary inflammatory injury related proteins expression and pulmonary pro-inflammatory cytokines content were determined after 42 d of feeding. The results showed that the Lactobacillus salivarius supplementation could improve growth performance and feed conversion ratio of broilers challenged with MG (P<0.01). In addition, the Lactobacillus salivarius supplementation could promote MG clearance in lung and alleviate lung injury caused by MG which were characterized by the significant decreased TLR2, HMGB1, p-p65/p65, NLRP3, Pro-Caspase-1/Caspase-1 expression levels (P<0.01) and significant decreased TNF-α, IL-1β, IL-6, IL-8 contents (P<0.01). These results suggested a tremendous potential of Lactobacillus salivarius as a feed additive in the poultry feed to against MG infection.

Key words: Mycoplasma gallisepticum, Lactobacillus salivarius, growth performance, lung injury

中图分类号:

S856.3
S855

王剑, 陈雪苹, 李继昌. 唾液乳杆菌对鸡毒支原体感染肉鸡生长性能及肺损伤的影响[J]. 畜牧兽医学报, 2022, 53(9): 3172-3179.

WANG Jian, CHEN Xueping, LI Jichang. Effects of Lactobacillus salivary on Growth Performance and Lung Injury of Broilers Challenged with Mycoplasma gallisepticum[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3172-3179.

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