H2S暴露对保育猪氧化还原状态及硫化氢代谢的影响

doi:10.11843/j.issn.0366-6964.2022.09.021

摘要/Abstract

摘要： 本试验旨在研究硫化氢（H₂S）暴露对保育猪氧化还原状态及内源性H₂S代谢的影响。试验选取12头体况健康、体重相近（（11.61±1.51） kg）的35日龄大白猪，随机分为2组并分配到2个环控舱内，公母各半，每组6头猪。试验组环控舱内H₂S浓度控制为30 mg·m^-3，对照组环控舱内H₂S浓度控制为0 mg·m^-3，试验期28 d。试验结束后采集血清和肝组织样品，检测氧化还原和H₂S代谢相关指标。结果显示，与对照组相比：1）血清中ROS含量极显著增加（P<0.01），MDA含量显著增加（P<0.05）；抗氧化酶T-SOD活性显著降低（P<0.05），CAT和GPX活性极显著降低（P<0.01）；非酶抗氧化物GSH和GSSG含量无显著变化（P>0.05）。2）肝中T-SOD和GPX活性极显著升高（P<0.01），·OH清除能力显著提高（P<0.05）；ROS、H₂O₂、PC、MDA、GSH、GSSG含量无显著差异（P>0.05）。3）肝中Keap1的mRNA表达量显著下调（P<0.05），Nrf2的mRNA表达量显著上调（P<0.05）；抗氧化相关基因（SOD2、GPX1、GPX2、GPX4和GSR）的mRNA表达量显著上调（P<0.05）。4）血清和肝中H₂S含量显著降低（P<0.05）；肝内源H₂S合成酶CSE和CBS的mRNA表达量显著下调（P<0.05），3-MST的mRNA表达量无显著变化（P>0.05）；肝H₂S分解代谢酶SQR和SUOX的mRNA表达量下调，但差异不显著（P>0.05）。结果表明，30 mg·m^-3 H₂S暴露导致保育猪血清抗氧化系统受损，而肝中Nrf2/Keap1信号通路的激活使肝免受氧化损伤；此外，H₂S暴露抑制了保育猪内源性H₂S的合成代谢。

关键词: 硫化氢(H₂S), 保育猪, 氧化还原状态, 代谢

Abstract: This study aimed to investigate the effects of hydrogen sulfide (H₂S) exposure on redox status and endogenous H₂S metabolism in nursery pigs. Twelve 35-day-old healthy Large White pigs with similar body weight ((11.61±1.51) kg) were randomly divided into two artificial environmental cabins with 6 pigs in each group, half male and half female per group. Pigs in the experimental group were exposed to 30 mg·m^-3 H₂S for 28 days, while pigs in the control group were kept without H₂S. Serum and liver samples were collected from each pig at the end of the experiment to detect the indexes related to redox and H₂S metabolism. The results showed that compared with the control group:1) In serum, the content of ROS increased extremely significantly (P<0.01) and MDA increased significantly (P<0.05); The activity of the antioxidant enzyme T-SOD decreased significantly (P<0.05), the activity of CAT and GPX decreased extremely significantly (P<0.01); The contents of non-enzymatic antioxidant GSH and GSSG did not change significantly (P>0.05). 2) In liver, the activities of T-SOD and GPX increased extremely significantly (P<0.01); the ability of scavenging ·OH improved significantly (P<0.05); The contents of ROS, H₂O₂, PC, MDA, GSH and GSSG showed no significant difference (P>0.05). 3) In liver, the mRNA expression of Keap1 was significantly down-regulated (P<0.05) and Nrf2 was significantly up-regulated (P<0.05); The mRNA expression of the antioxidant-related gene (SOD2, GPX1, GPX2, GPX4 and GSR) were significantly up-regulated (P<0.05). 4) The concentration of H₂S in serum and liver decreased significantly (P<0.05); The mRNA expressions of endogenous H₂S synthase CSE and CBS in the liver were significantly down-regulated (P<0.05) while that of 3-MST was not significantly different (P>0.05), and the mRNA expressions of H₂S catabolic enzyme SQR and SUOX showed no significant change either (P>0.05). The results indicated that 30 mg·m^-3 H₂S exposure caused damage to the serum antioxidant system of nursery pigs, but the activation of the Nrf2/Keap1 signaling pathway in liver protected the liver from oxidative damage. In addition, H₂S exposure inhibited the anabolism of endogenous H₂S in nursery pigs.

Key words: hydrogen sulfide (H₂S), nursery pigs, redox status, metabolism

中图分类号:

陈磊, 刘真, 谢彦娇, 苗启翔, 骆城增, 张宏福, 唐湘方. H₂S暴露对保育猪氧化还原状态及硫化氢代谢的影响[J]. 畜牧兽医学报, 2022, 53(9): 3052-3062.

CHEN Lei, LIU Zhen, XIE Yanjiao, MIAO Qixiang, LUO Chengzeng, ZHANG Hongfu, TANG Xiangfang. Effects of H₂S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3052-3062.

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H₂S暴露对保育猪氧化还原状态及硫化氢代谢的影响

Effects of H₂S Exposure on Redox Status and Hydrogen Sulfide Metabolism in Nursery Pigs

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