基于血浆代谢组学筛选日粮纤维影响二花脸猪肉质性状的候选代谢物

doi:10.11843/j.issn.0366-6964.2023.09.016

摘要/Abstract

摘要： 本课题组前期研究发现,7%的麸皮替代基础日粮可以显著改善二花脸猪背最长肌红度值,提高其肉品质,但其潜在的生理和分子调控机理未知。旨在利用非靶向代谢组学技术,鉴别日粮纤维影响二花脸猪肉质性状的候选代谢物;并利用二花脸猪肌管细胞体外探究日粮纤维影响二花脸猪肉品质的潜在生理和分子调控机理。本试验利用非靶向代谢组学技术检测基础日粮组和7%麸皮替代基础日粮组二花脸猪血浆样品的差异代谢物,并与肉质表型数据进行联合分析,鉴别日粮纤维影响二花脸猪肉质性状的候选代谢物。然后利用候选代谢物处理体外分离培养的二花脸猪肌管细胞,研究其对肌纤维类型转变的影响。结果显示,在基础日粮组和7%麸皮替代基础日粮组之间共鉴定到9个差异代谢物,其中4个差异代谢产物在基础日粮组含量较高,5个差异代谢产物在7%麸皮替代基础日粮组含量较高。KEGG通路分析显示,二花脸猪的血浆差异代谢产物主要富集在黑色素生成途径、ABC转运体途径、酪氨酸和色氨酸生物合成途径等代谢途径上。通过与前期研究获得的肉质表型数据进行相关性分析发现,血浆差异代谢物十七烷酸与二花脸猪背最长肌24 h 红度值(a_{24 h})呈显著正相关。体外细胞研究表明,十七烷酸处理二花脸猪肌管细胞,提高了I型肌纤维类型标记基因 MyHC I的表达水平。综上,适量的日粮纤维添加改变了二花脸猪血浆中十七烷酸的水平,进而通过提高 MyHC I基因的表达改善二花脸猪背最长肌的肉色,提高其猪肉品质。

关键词: 二花脸猪, 日粮纤维, 血浆代谢组学, 十七烷酸

Abstract: Previous study in authors' research group have found that 7% bran replacement basal diet can significantly improve the redness value of Longissimus dorsi muscle of Erhualian pigs and improve their meat quality, but its potential physiological and molecular regulatory mechanisms remain unknown. The aim of the study was to identify the candidate metabolites of dietary fiber affecting meat quality traits of Erhualian pigs using non-targeted metabolomics technology and to explore the potential physiological and molecular regulatory mechanism of dietary fiber affecting the meat quality using an in vitro myotubes derived from Erhualian pigs. Differential plasma metabolites between basal diet group and 7% bran replacement basal diet group were detected by non-targeted metabolomics technology in Erhualian pigs, then the correlation between differential plasma metabolites and meat quality phenotype data were analyzed to identify dietary fiber candidate metabolites affecting the meat quality traits of Erhualian pigs. Then the candidate metabolite was used to treat Erhualian pig myotubes in vitro to study its effect on the transformation of muscle fiber types. The results showed that, a total of 9 differential metabolites were identified between the basal diet group and the 7% bran replacement basal diet group, of which 4 differential metabolites had higher level in the basal diet group and 5 differential metabolites had higher level in the 7% bran replacement basal diet group. KEGG pathway analysis showed that plasma differential metabolites of Erhualian pigs were mainly involved in melanin generation pathway, ABC transporter pathway and tyrosine and tryptophan biosynthesis pathway, etc. The plasma metabolite heptadecanoic acid was found to be positively correlated with the 24 h redness value (a_{24 h}) of Longissimus dorsi muscle of Erhualian pigs by correlation analysis with the meat quality phenotype data obtained in previous studies. The results of in vitro cell experiments showed that the expression level of type I muscle fiber type marker gene MyHC I was increased by heptadecanoic acid treatment in Erhualian pig myotube cells. In summary, appropriate dietary fiber supplementation changed the plasma metabolome of Erhualian pigs, and heptadecanoic acid may improve the flesh color of Longissimus dorsi muscle of Erhualian pigs by increasing the expression of MyHC I gene and thus improved the quality of pork.

Key words: Erhualian pigs, dietary fiber, plasma metabolomics, heptadecanoic acid

中图分类号:

S828.2

张兆博, 侯黎明, 李平华, 杜陶然, 王中宇, 吴承武, 黄瑞华. 基于血浆代谢组学筛选日粮纤维影响二花脸猪肉质性状的候选代谢物[J]. 畜牧兽医学报, 2023, 54(9): 3758-3769.

ZHANG Zhaobo, HOU Liming, LI Pinghua, DU Taoran, WANG Zhongyu, WU Chengwu, HUANG Ruihua. Screening Candidate Metabolites of Dietary Fiber Affecting Meat Quality Traits of Erhualian Pigs Based on Plasma Metabolomics[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3758-3769.

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