黄体期注射PGF2α对母羊血清生殖激素及相关细胞因子的影响

doi:10.11843/j.issn.0366-6964.2022.06.014

摘要/Abstract

摘要： 旨在研究黄体期不同阶段注射前列腺激素(PGF2α)对育成母羊生殖激素和生殖相关细胞因子的影响。本研究选择健康、体况良好、体重相近、发情周期正常的湖羊育成母羊60只，用“孕酮栓(MAP)+PMSG”法进行发情周期同步化处理后，选择发情正常的48只母羊随机均分为6组。发情当天记为第0天，黄体前期试验组、中期试验组和末期试验组母羊分别在第6(黄体前期)、11(黄体中期)、16天(黄体末期)注射1 mL PGF2α(0.1 mg)，黄体前期对照组、中期对照组和末期对照组母羊分别在第6、11、16天注射1 mL生理盐水，每次注射后0.5、1、2、3 h采血，用于血液指标检测。结果表明，所有试验组和对照组母羊于注射后的0.5~3 h间血清中FSH、LH、PRL、P₄、E₂水平以及TNF-α、IL-1β、IL-6、IFN-β无显著变化(P＞0.05)；母羊在黄体期不同阶段注射PGF2α对0.5、1、2、3 h血清中FSH、LH、PRL及IL-1β、IFN-β无显著影响(P＞0.05)，前期试验组注射后3 h P₄水平显著低于前期对照组，E₂和IL-6水平显著高于前期对照组(P＜0.05)，前期试验组注射后2和3 h TNF-α水平显著高于前期对照组(P＜0.05)；中期试验组注射后1 h P₄水平显著低于中期对照组(P＜0.05)。黄体前期注射PGF2α后，前期试验组0.5~ 3 h内FSH、E₂、TNF-α、IL-6整体水平显著高于前期对照组(P＜0.05)，P₄整体水平显著低于前期对照组(P＜0.05)，对LH、PRL、IL-1β和IFN-β无显著影响(P＞0.05)；黄体中期注射PGF2α后，中期试验组0.5~3 h内E₂整体水平显著高于中期对照组(P＜0.05)，P₄整体水平显著低于中期对照组(P＜0.05)，对FSH、LH、PRL、TNF-α、IL-1β、IL-6和IFN-β无显著影响(P＞0.05)；黄体末期注射PGF2α对生殖激素与相关细胞因子没有显著性影响(P＞0.05)。本研究结果表明，PGF2α对黄体的溶解作用存在阶段性差异，母羊在黄体前期对PGF2α的短期应答反应强于黄体中、末期，且黄体前期时，卵巢可以响应PGF2α为卵泡发育营造更佳的发育环境。

关键词: 湖羊, 黄体期, 生殖激素, 炎症因子

Abstract: The aim of this study was to investigate the effects of prostaglandin F2α (PGF2α) injection at different phases of luteal on reproductive hormones and related cytokines in growing ewes. A total of 60 healthy ewes in good condition with similiar weight and normal estrous cycle were used in this study. Forty eight ewes with normal estrus were randomly divided into 6 groups after synchronization of estrus cycle with "MAP +PMSG" method. The day of oestrus was recorded as day 0. Ewes in the experiment groups of early-, mid-, and late-luteal phase were injected with 1 mL PGF2α (0.1 mg) on day 6 (early-luteal phase), 11 (mid-luteal phase) and 16 (late-luteal phase) of luteal phase, respectively. Ewes in the control groups of different luteal phases were injected with 1 mL normal saline on day 6, 11 and 16 of luteal phase, respectively. Blood was collected at 0.5, 1, 2 and 3 hours after each injection for the determination of blood indexes. The results showed as follows: There were no significant differences in levels of FSH, LH, PRL, P₄, E₂, TNF-α, IL-1β, IL-6 and IFN-β between experimental groups and control groups at 0.5, 1, 2, 3 h after injection (P > 0.05). Injection of PGF2α at different phases of luteal had no significant effect on serum FSH, LH, PRL, IL-1β and IFN-β levels at 0.5, 1, 2 and 3 h (P > 0.05). At 3 h after injection, P₄ level was significantly lower, E₂ and IL-6 levels were significantly higher in the early-luteal experiment group than that in control group (P < 0.05). The level of TNF-α in the early-luteal experiment group was significantly higher than that in control group at 2 and 3 h after injection (P < 0.05), and the level of P₄ in the mid-luteal experiment group was significantly lower than that in the control group at 1 h after injection (P < 0.05). After injection of PGF2α in the early-luteal phase, the overall levels of FSH, E₂, TNF-α and IL-6 in the early-luteal experiment group were significantly higher than those in control group within 3 h (P < 0.05), and the overall level of P₄ was significantly lower than that in control group (P < 0.05), there were no significant effects on LH, PRL, IL-1β and IFN-β (P>0.05). After injection of PGF2α in the mid-luteal phase, the overall level of E₂ in the mid-luteal experiment group was significantly higher than that in control group within 3 h(P < 0.05), and P₄ was significantly lower than that in control group (P < 0.05), there were no significant effects on FSH, LH, PRL, TNF-α, IL-1β, IL-6 and IFN-β (P>0.05). Injection of PGF2α at the late-luteal had no significant effect on reproductive hormones and related cytokines (P>0.05). In conclusion, there are stage differences of PGF2α in dissolving corpus luteum. The short-term response of ewes to PGF2α was stronger in early-luteal phase than in mid- and late-luteal phases. The ovaries could respond to PGF2α in early-luteal phase to create a better environment for follicular development.

Key words: Hu sheep, luteal phase, reproductive hormones, inflammatory factors

中图分类号:

S826.3

李宇, 段春辉, 宋志攀, 岳思聪, 王媛, 张英杰, 刘月琴. 黄体期注射PGF2α对母羊血清生殖激素及相关细胞因子的影响[J]. 畜牧兽医学报, 2022, 53(6): 1807-1818.

LI Yu, DUAN Chunhui, SONG Zhipan, YUE Sicong, WANG Yuan, ZHANG Yingjie, LIU Yueqin. Effects of PGF2α on Reproductive Hormones and Related Cytokines during Luteal Phase in Ewes[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1807-1818.

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