体细胞克隆技术在乌金猪遗传资源保护上的应用

doi:10.11843/j.issn.0366-6964.2020.09.010

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (9): 2130-2137.doi: 10.11843/j.issn.0366-6964.2020.09.010

体细胞克隆技术在乌金猪遗传资源保护上的应用

卯升义^1,2, 李卓^1,2, 奎华², 角德灵^1,2, 李钰莹^1,2, 卿玉波^1,3, 郭建雄¹, 韦云芳^1,2, 魏红江^1,3*

1. 云南省动物基因编辑与体细胞克隆技术重点实验室, 昆明 650201;
2. 云南农业大学动物科学技术学院, 昆明 650201;
3. 云南农业大学动物医学院, 昆明 650201

收稿日期:2020-02-18 出版日期:2020-09-25 发布日期:2020-09-25
通讯作者: 魏红江,主要从事动物基因编辑与体细胞克隆技术研究,E-mail:hongjiangwei@126.com
作者简介:卯升义(1993-),男,云南昭通人,硕士生,主要从事动物营养与饲料科学研究,E-mail:shengyim24@163.com;李卓(1994-),男,湖南长沙人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:zhuoli2018@163.com。
基金资助:
云南省生物医药重大研发平台构建（2018ZF018）；云南省院士（专家）工作站（2018IC064）

Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs

MAO Shengyi^1,2, LI Zhuo^1,2, KUI Hua², JIAO Deling^1,2, LI Yuying^1,2, QING Yubo^1,3, GUO Jianxiong¹, WEI Yunfang^1,2, WEI Hongjiang^1,3*

1. Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming 650201, China;
2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
3. College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China

Received:2020-02-18 Online:2020-09-25 Published:2020-09-25

摘要/Abstract

摘要： 旨在通过猪体细胞克隆技术，生产乌金猪火毛系，并分析生长发育能力及繁殖性能，为该技术在地方优良猪种保种上的应用提供理论依据。本研究采集了符合乌金猪种质特征的3头公猪和12头母猪的耳组织样品。公猪年龄分别为3、6、11月龄，母猪年龄差距较大，最小的2月龄，最大的10岁。其中3头母猪和1头3月龄公猪已被去势，10岁母猪已无繁殖能力。结果，成功建立了15头乌金猪的耳组织成纤维细胞系，分别选择1头3月龄乌金猪（♂）和10岁乌金猪（♀）的成纤维细胞进行体细胞核移植，经胚胎移植入16头代孕母猪，共获得25头克隆猪，克隆猪生长发育正常，性成熟后进行自然交配共获得39头F1代活仔。本研究通过体细胞克隆技术成功克隆了乌金猪，具备正常的生长发育性能和繁殖性能，为地方猪种的保护研究提供了新的途径。

关键词: 乌金猪, 体细胞克隆技术, 生长性能, 繁殖性能

Abstract: The purpose of this experiment was to study the growth and developmental ability and reproductive performance of cloned Wujin pigs fire hair line by somatic cell cloning technology, and provide a theoretical basis for the application of somatic cell cloning technology in the conservation of local excellent pig breeds. In this study, the ear samples were collected from 3 male and 12 female pigs which met the characteristics of Wujin pigs. The 3 boars were 3, 6 and 11 months old, respectively. The sows had a large age gap, from 2 months old to 10 years old, the three sows and one boar of 3-month-old had been castrated and the sow of 10-year-old had no reproductive capacity. The ear fibroblast cell lines were successfully established from 15 Wujin pigs. Then the fibroblast cells from 3-month-old(♂) and 10-year-old(♀) pigs were used as donor cells for somatic cell nuclear transfer. The cloned embryos were transplanted into 16 surrogate sows and 25 cloned pigs were obtained. The growth and development indexes of these cloned pigs were all normal. After sexual maturity, the male and female cloned pigs mated naturally and 39 live piglets (F1) were obtained. This study successfully cloned Wujin pigs by somatic cell nuclear transfer technology, and cloned Wujin pigs had normal growth and developmental ability and reproductive performance. The study result provides a new way for the conservation of local pig breeds.

Key words: Wujin pigs, somatic cell cloning technology, growth performance, reproductive performance

中图分类号:

S828.3

卯升义, 李卓, 奎华, 角德灵, 李钰莹, 卿玉波, 郭建雄, 韦云芳, 魏红江. 体细胞克隆技术在乌金猪遗传资源保护上的应用[J]. 畜牧兽医学报, 2020, 51(9): 2130-2137.

MAO Shengyi, LI Zhuo, KUI Hua, JIAO Deling, LI Yuying, QING Yubo, GUO Jianxiong, WEI Yunfang, WEI Hongjiang. Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2130-2137.

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体细胞克隆技术在乌金猪遗传资源保护上的应用

Application of Somatic Cell Cloning Technology in the Protection of Genetic Resources of Wujin Pigs

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