畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (4): 649-659.doi: 10.11843/j.issn.0366-6964.2020.04.001
徐小丽1,2, 曹家雪1,2*
收稿日期:
2019-11-01
出版日期:
2020-04-25
发布日期:
2020-04-21
通讯作者:
曹家雪,主要从事羊遗传育种研究,E-mail:jiaxuecao@sicau.edu.cn
作者简介:
徐小丽(1995-),女,四川泸州人,硕士生,主要从事山羊遗传育种研究,E-mail:xxl18428396995@163.com
基金资助:
XU Xiaoli1,2, CAO Jiaxue1,2*
Received:
2019-11-01
Online:
2020-04-25
Published:
2020-04-21
摘要: CRISPR/Cas9系统是一种广泛存在于细菌和古菌中的免疫机制。近年来,已发展为一种快捷高效的基因编辑工具,用于研究编码或非编码RNA的功能。非编码RNA是一类不编码蛋白质的RNA,其可通过多种调控途径在动物的生长发育、疾病免疫等生理或病理过程中发挥重要的生物学功能。CRISPR/Cas9技术可以靶向核酸序列稳定敲除基因,得到敲除小鼠或细胞系,虽然其在非编码RNA功能研究中的使用干扰了邻近基因或宿主基因表达,但该技术的出现为非编码RNA功能机制的探索提供了不同的途径。本文通过简要概述CRISPR/Cas系统的发展和作用原理,并重点介绍CRISPR/Cas9技术在动物miRNA、lncRNA及circRNA功能研究中的应用,以期为相关研究提供参考。
中图分类号:
徐小丽, 曹家雪. CRISPR/Cas9技术在动物非编码RNA功能研究中的应用[J]. 畜牧兽医学报, 2020, 51(4): 649-659.
XU Xiaoli, CAO Jiaxue. Application of CRISPR/Cas9 Technology in the Study of Animal Non-coding RNA Function[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 649-659.
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