饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析

doi:10.11843/j.issn.0366-6964.2024.01.022

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (1): 232-244.doi: 10.11843/j.issn.0366-6964.2024.01.022

饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析

张德安¹, 杨若渚¹, 刘杰¹, 刘德武^1,2,3, 邓铭¹, 柳广斌^1,2,3, 孙宝丽^1,2,3, 郭勇庆^1,2,3, 李耀坤^1,2,3*

1. 华南农业大学动物科学学院, 广州 510642;
2. 华南农业大学畜禽育种国家地方联合工程研究中心, 广州 510642;
3. 华南农业大学广东省农业动物基因组学与分子育种重点实验室, 广州 510642

收稿日期:2023-05-26 出版日期:2024-01-23 发布日期:2024-01-24
通讯作者: 李耀坤,主要从事遗传育种与繁殖及智慧养殖研究,E-mail:ykli@scau.edu.cn
作者简介:张德安(1999-),男,湖北宜昌人,硕士,主要从事动物健康养殖与安全生产研究,E-mail:zhangdean1999@163.com;杨若渚(1999-),女,广东揭阳人,硕士,主要从事动物遗传育种与繁殖研究,E-mail:2381519141@qq.com。张德安和杨若渚为同等贡献作者
基金资助:
石山羊的遗传选育和高效养殖技术示范推广应用（KTP20210344）；广东省现代农业产业技术体系项目（2021KJ127）

Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn

ZHANG De'an¹, YANG Ruozhu¹, LIU Jie¹, LIU Dewu^1,2,3, DENG Ming¹, LIU Guangbin^1,2,3, SUN Baoli^1,2,3, GUO Yongqing^1,2,3, LI Yaokun^1,2,3*

1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. National Engineering Research Center for Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China;
3. Guangdong Key Laboratory of Agricultural Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China

Received:2023-05-26 Online:2024-01-23 Published:2024-01-24

摘要/Abstract

摘要： 旨在分析饲喂青贮黄梁木替代50%青贮玉米的川中黑山羊肝转录组测序中差异表达的mRNA、lncRNA及其靶基因，挖掘饲喂青贮黄梁木影响川中黑山羊肝代谢的关键基因，从而探究青贮黄粱木的饲喂效果。试验共选取6只雄性健康川中黑山羊[5月龄，平均体重（21.07±4.05） kg]并随机分为两组，试验组为青贮黄梁木替代50%青贮玉米组，对照组为青贮玉米组。试验期间采用全混合日粮（TMR）进行饲喂。于试验期的最后1 d对山羊进行屠宰并取其肝，提取肝组织的RNA，构建文库后使用高通量测序技术进行转录组测序，以P<0.05为阈值筛选出差异mRNA和lncRNA，进行生物学功能富集分析并构建靶向关系网络。再从差异表达lncRNA和mRNA中各随机选择3个基因，采用RT-qPCR进行验证分析。结果表明，共发现差异表达的mRNA有1 696个，其中943个上调，753个下调；差异表达lncRNA共有33个，其中22个上调，11个下调。结合差异表达lncRNA的靶基因与差异表达mRNA的交集基因的富集分析发现，NDUFA10、NDUFB11、NDUFS5参与肝功能相关的信号通路，如多糖代谢过程、葡聚糖分解代谢过程、纤维素分解代谢等。结合lncRNA靶基因预测发现，GSTA3、GSTA4基因参与肝功能相关的信号通路如药物代谢-细胞色素P450及细胞色素P450对异源物质的代谢等代谢通路。本研究发现，在饲喂青贮黄梁木川中黑山羊的肝组织中，NDUFA10、NDUFB11、NDUFS5以及GSTA3、GSTA4等可能从营养物质代谢和毒素代谢等方面影响肝功能。

关键词: 青贮黄粱木, 川中黑山羊, 肝, lncRNA, mRNA

Abstract: The purpose of this study was to analyze the differentially expressed mRNA, lncRNA and its target genes in liver transcripome sequencing of Chuanzhong Black Goats fed silage Neolamarckia Cadamba instead of 50% silage corn, and to find out the key genes affecting liver metabolism of Chuanzhong Black Goats fed silage Neolamarckia Cadamba, so as to explore the feeding effect of silage Neolamarckia Cadamba. A total of 6 healthy male Chuanzhong Black Goats [5 months old, average body weight (21.07±4.05) kg] were selected and randomly divided into two groups: experimental group was fed silage Neolamarckia Cadamba replaced 50% silage corn, and control group was fed silage corn. Total mixed diet (TMR) was used for feeding during the experiment. On the last day of the experiment period, goats were slaughtered and their livers were taken. RNA from the liver tissues was extracted to construct a library. High-throughput sequencing technology was used for transcriptome sequencing. Differential mRNA and lncRNA were screened at a threshold of P<0.05, and biological function enrichment analysis was conducted to construct a targeted relationship network. Finally, 3 genes were randomly selected from differentially expressed lncRNA and mRNA separately, and verified by RT-qPCR. The results showed that a total of 1 696 mRNA were differentially expressed, of which 943 were up-regulated and 753 were down-regulated. A total of 33 lncRNA were differentially expressed, of which 22 were up-regulated and 11 down-regulated. Combined with the enrichment analysis of target genes of differentially expressed lncRNA and intersection genes of differentially expressed mRNA, it was found that NDUFA10, NDUFB11 and NDUFS5 were involved in signal pathways related to liver function, such as polysaccharide metabolism, glucan catabolism and cellulose catabolism. Combined with lncRNA target gene prediction, it was found that GSTA3 and GSTA4 genes were involved in signal pathways related to liver function, such as drug metabolism-cytochrome P450 and cytochrome P450 metabolism of heterologous substances. In conclusion, NDUFA10, NDUFB11, NDUFS5, GSTA3 and GSTA4 may affect the liver function from the aspects of nutrient metabolism and toxin metabolism in the liver tissue of Chuanzhong Black Goats fed with silage Neolamarckia Cadamba.

Key words: silage Neolamarckia Cadamba, Chuanzhong Black Goat, liver, lncRNA, mRNA

中图分类号:

S813

张德安, 杨若渚, 刘杰, 刘德武, 邓铭, 柳广斌, 孙宝丽, 郭勇庆, 李耀坤. 饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析[J]. 畜牧兽医学报, 2024, 55(1): 232-244.

ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.

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饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析

Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn

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