不同添加水平的磷酸一二钙对肉鸡生产性能、钙磷代谢及盲肠微生物的影响

doi:10.11843/j.issn.0366-6964.2020.014

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (6): 1306-1319.doi: 10.11843/j.issn.0366-6964.2020.014

不同添加水平的磷酸一二钙对肉鸡生产性能、钙磷代谢及盲肠微生物的影响

刘景¹, 陈炳钿^1,2, 李忠荣¹, 何姝颖³, 张世忠¹, 邱华玲¹, 缪伏荣¹, 陈卓⁴, 陈鑫珠^1,2*

1. 福建省农业科学院畜牧兽医研究所, 福州 350013;
2. 福建省新闽科生物科技开发有限公司, 福州 350008;
3. 中化云龙有限公司, 成都 655204;
4. 福建商学院工商管理学院, 福州 350016

收稿日期:2020-01-07 出版日期:2020-06-25 发布日期:2020-06-23
通讯作者: 陈鑫珠,主要从事动物营养与饲料科学方面的研究,E-mail:010622051@163.com
作者简介:刘景(1967-),男,福建长乐人,副研究员,本科,主要从事动物营养需要及资源化利用研究,E-mail:184660569@qq.com
基金资助:
省属公益类科研院所（2016R1022-4）；福建省农业科学院对外合作项目（DEC201909）

Effects of Different Dietary Mono-dicalcium Phosphate Supplementation on Production Performance, Blood Parameters, Bone Calcium, Phosphorus Metabolism and Cecal Microecology in Broilers

LIU Jing¹, CHEN Bingdian^1,2, LI Zhongrong¹, HE Shuying³, ZHANG Shizhong¹, QIU Hualing¹, MIAO Furong¹, CHEN Zhuo⁴, CHEN Xinzhu^1,2*

1. Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. Fujian Xinminke Biology Science and Technology Development Co., Ltd., Fuzhou 350008, China;
3. Sinochem Yunlong Co., Ltd., Chengdu 655204, China;
4. Business Administration College, Fujian Business University, Fuzhou 350016, China

Received:2020-01-07 Online:2020-06-25 Published:2020-06-23

摘要/Abstract

摘要： 本试验旨在研究磷酸一二钙（MDCP）对AA肉鸡生产性能、血液生化指标、胫骨强度、骨骼中钙、磷代谢和盲肠微生态的影响以及在饲粮中完全替代磷酸氢钙的适宜磷酸一二钙添加水平。试验选取健康的1日龄AA肉鸡450只，随机分为5组。对照组（CK）以磷酸氢钙（DCP）补充饲粮非植酸磷，试验组以MDCP补充饲粮非植酸磷，试验1组至试验4组饲粮中非植酸磷水平分别为对照组的60%、80%、100%及120%，即T1、T2、T3和T4。每组6个重复，每个重复15只，试验期42 d。于试验第19~21天和第39~41天进行代谢试验，于第21、42天分别进行生产性能、血液、盲肠微生物等指标测定和样品采集及分析。结果表明：1）与对照组相比，不同试验组均显著提高21 d和42 d肉鸡的生产性能（P<0.05），其中试验2组欧洲指数最高；2）除试验1组钙表观代谢率外，其他3个试验组21 d钙、磷的表观代谢率显著降低（P<0.05），试验1组和试验2组42 d的钙、磷表观代谢率显著提高（P<0.05）；3）试验1组21 d胫骨的钙含量较对照组显著升高（P<0.05），磷和最大应力显著降低（P<0.05），试验3组42 d胫骨的钙和磷含量较对照组和试验1组显著提高（P<0.05），试验2组和试验3组的最大应力显著高于其他处理组（P<0.05）；4）试验1组21 d血磷含量较对照组显著降低（P<0.05），试验4组较对照组显著提高（P<0.05）；5）肉鸡盲肠微生物高通量测序结果表示，对照组和4个试验组21 d肉鸡盲肠微生物有明显差异，聚类分支距离较远，42 d肉鸡试验组的微生物多样性较对照组丰富，其多样性明显高于21 d。综合各项指标结果表明，添加MDCP磷源的量为DCP磷源量的80%时，肉鸡1~21和1~42 d的生产性能，血液、骨骼指标均能达到最佳效果，同时有助于肠道微生物的稳定和健康。

关键词: 肉鸡, 磷酸一二钙, 磷酸氢钙, 生产性能, 血液生化指标, 盲肠微生物

Abstract: The purpose of this experiment was to study the effects of mono-dicalcium phosphate (MDCP) on growth performance, serum biochemical indices, tibia indices, metabolism of calcium and phosphorous in bone and cecal microecology of AA broilers and to evaluate the appropriate level of addition for the complete replacement of dicalcium phosphate (DCP) with MDCP in diet. A total of 450 one-day old AA broilers were randomly divided into 5 groups. DCP was used as the control group(CK). Four levels of MDCP were used to replace DCP to provide non-phytate phosphorus which was provided 60%, 80%, 100%, and 120% of the total non-phytate phosphorus labeled as treatment 1 (T1), treatment 2(T2), treatment 3(T3) and treatment 4(T4), respectively, with 6 replicates per group and 15 broilers per replicate for a 42-day trial period. Metabolic traits were conducted from day 19 to 21 and day 39 to 41, respectinely. Data of production performance, blood samples, and cecal microbes were collected and analyzed on 21 and 42 d, respectively. The results showed as follows:1) Compared with the CK group, the MDCP test groups improved the performance of broilers at 21 and 42 d significantly (P<0.05), and T2 group had the highest European index. 2) The apparent metabolic rate of calcium and phosphorus were decreased significantly in T2, T3 and T4 groups (P<0.05) at 21 d, and was increased significantly in T1 and T2 groups at 42 d (P<0.05). 3) Tibia calcium content of T1 group was significantly increased and phosphorus and maximum stress were significantly decreased at 21 d compared with CK group (P<0.05), tibia calcium and phosphorus content of T3 group were significantly increased compared with CK and T1 group at 42 d (P<0.05), the maximum stress of T2 and T3 group were significantly higher than the other groups at 42 d (P<0.05). 4) Compared with CK group, the blood phosphorus content was significantly decreased in T1 group (P<0.05), and was significantly increased in T4 group (P<0.05). 5) The results of high-throughput sequencing of cecal microbes in broiler chickens showed that there were significant differences in cecal microbes between the CK group and the T1-T4 groups, and the clustering distance was farther at 21 d. The diversity of cecal microbes at 42 d was significantly higher than at 21 d. The results of various indicators showed that MDCP could replace DCP as a source of non-phytate phosphorus in broiler chickens. According to European index, tibia indices, serum biochemical indices and cecal microbes of broilers, the MDCP at 80% replacement level had the best performance.

Key words: broiler, mono-dicalcium phosphate (MDCP), dicalcium phosphate (DCP), growth performance, serum biochemical indices, cecal microbes

中图分类号:

S816.71

刘景, 陈炳钿, 李忠荣, 何姝颖, 张世忠, 邱华玲, 缪伏荣, 陈卓, 陈鑫珠. 不同添加水平的磷酸一二钙对肉鸡生产性能、钙磷代谢及盲肠微生物的影响[J]. 畜牧兽医学报, 2020, 51(6): 1306-1319.

LIU Jing, CHEN Bingdian, LI Zhongrong, HE Shuying, ZHANG Shizhong, QIU Hualing, MIAO Furong, CHEN Zhuo, CHEN Xinzhu. Effects of Different Dietary Mono-dicalcium Phosphate Supplementation on Production Performance, Blood Parameters, Bone Calcium, Phosphorus Metabolism and Cecal Microecology in Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1306-1319.

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不同添加水平的磷酸一二钙对肉鸡生产性能、钙磷代谢及盲肠微生物的影响

Effects of Different Dietary Mono-dicalcium Phosphate Supplementation on Production Performance, Blood Parameters, Bone Calcium, Phosphorus Metabolism and Cecal Microecology in Broilers

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