牛体内囊胚全转录组模式解析

doi:10.11843/j.issn.0366-6964.2020.011

摘要/Abstract

摘要： 旨在解析牛体内囊胚的全转录组模式。本研究选择3头15月龄以上、健康状况良好的泌乳奶牛，采用超排获得体内囊胚，采用单细胞全转录组测序技术检测囊胚mRNA、lncRNA、circRNA转录模式，并采用生物信息学工具对其进行分析。试验获得牛体内囊胚约19 975个mRNAs、12 715个novel lncRNAs及887个circRNAs。mRNA有12种可变剪切方法，以转录起始区域可变剪切（TSS）为主。采用基因本体（GO）和相关信号通路（KEGG）对其分析发现，生物功能主要富集在新陈代谢、细胞膜和蛋白结合、细胞通讯、细胞组织成分、生长发育等功能，Pathway主要富集在细胞周期、膜运输、染色体组织调控等通路；circRNA有6种类型，其中CLASSIC类型circRNA数量最多，这种剪切方式会形成成环的外显子circRNA （EcircRNA）。本研究阐明了牛体内囊胚的全转录组模式，为全面了解牛体内囊胚提供了新的思路。

关键词: 牛体内囊胚, mRNA, lncRNA, circRNA

Abstract: The purpose of this study was to explore the whole transcription pattern of bovine in vivo blastocysts. Three lactating cows over 15-month-old and in good health were selected, superovulation technology was used to obtain in vivo blastocysts. Single-cell whole transcriptome sequencing technology was used to detect the transcription pattern of mRNA, lncRNA, and circRNA of bovine blastocyst, and bioinformatics tools were used to analyze the related informations. As a result of the experiment, bovine blastocysts had about 19 975 mRNAs, 12 715 novel lncRNAs, and 887 circRNAs. There were 12 alternative splicing methods for mRNAs, mainly the transcription start site (TSS). GO and KEGG were used to analyze mRNAs. It was found that biological functions were mainly enriched in metabolism, cell membrane and protein binding, cell communication, cellular component organization and developmental process in GO enrichment analysis; The pathways were enriched in cell cycle, membrane transport, and chromosome tissue regulation. There were 6 types of circRNAs, of which the CLASSIC type had the largest number. This type of splicing would form a circular exon circRNA (EcircRNA). The whole transcriptome pattern of bovine in vivo blastocyst was clarified in this study, which provided new ideas for a comprehensive understanding of bovine in vivo blastocysts.

Key words: in vivo bovine blastocyst, mRNA, lncRNA, circRNA

中图分类号:

S823.3

王晶晶, 张培培, 郝海生, 杜卫华, 庞云渭, 权国波, 赵善江, 邹惠影, 朱化彬, 赵学明. 牛体内囊胚全转录组模式解析[J]. 畜牧兽医学报, 2020, 51(6): 1271-1280.

WANG Jingjing, ZHANG Peipei, HAO Haisheng, DU Weihua, PANG Yunwei, QUAN Guobo, ZHAO Shanjiang, ZOU Huiying, ZHU Huabin, ZHAO Xueming. Analysis of Whole Transcriptome Pattern of Bovine in vivo Blastocyst[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1271-1280.

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