兔出血症病毒胶体金免疫层析试纸条诊断方法的建立及初步应用

Abstract

Abstract:

To establish a rapid, simple assay for rabbit hemorrhagic disease virus (RHDV), purified polyclone antibody against RHDV was labeled with colloidal gold particle. Monoclonal antibody (McAb) A3C against RHDV was prepared with recombinant RHDV VP60 protein expressed by baculovirus expression system. The detection zone and quality control zone of the nitrocellulose membrane was made by A3C and goat anti-rabbit IgG. The immune colloidal gold test strip was developed by optimizing the experiment conditions. The sensitive test showed that the test strip can detect the RHDV which erythrocyte agglutination test (HA) titer was 1∶10, which is negative result if the RHDV was detected by HA. It means that the test strip is more sensitive than erythrocyte agglutination test. The crossing test showed that the test strip could not react with other rabbit common pathogens. 127 clinical liver samples of rabbit were detected by the test strip, while a parallel experiment was conducted using HA, and the positive consistent rate of both was 100%. All of the results showed that the test strip is a rapid, sensitive and specific method for detecting RHDV infection. The method has provided a effective tool for diagnosing RHDV, particularly in clinical diagnosis.

CLC Number:

S855.3

CAI Shao-ping, WANG Fang, JIA Hua-min, ZHANG Hai-bin, LI Chao-mei, FAN Zhi-yu, HU Bo, XIONG Fu-qiang, WEI Hou-Jun. Development and Primary Application of an Immunochromatographic Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Development and Primary Application of an Immunochromatographic Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus

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